Vitamin D-protected bone marrow stromal cell from apoptosis induced by γ-irradiation

ZHANG Zengli

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Vitamin D-protected bone marrow stromal cell from apoptosis induced by γ-irradiation

更新时间：2023-03-30

- Vitamin D-protected bone marrow stromal cell from apoptosis induced by γ-irradiation
- Journal of Radiation Research and Radiation Processing Vol. 24, Issue 6, Pages: 367-369(2006)
- 作者机构：
  
  1.苏州大学放射医学与公共卫生学院　苏州　215123
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ZHANG Zengli. Vitamin D-protected bone marrow stromal cell from apoptosis induced by γ-irradiation. [J]. Journal of Radiation Research and Radiation Processing 24(6):367-369(2006)
DOI：

ZHANG Zengli. Vitamin D-protected bone marrow stromal cell from apoptosis induced by γ-irradiation. [J]. Journal of Radiation Research and Radiation Processing 24(6):367-369(2006) DOI：

摘要

本实验利用骨髓基质细胞（Bone marrow stromal cell，BMSC）克隆形成法、流式细胞仪分析BMSC凋亡、Western-blot分析BMSC内caspase-3蛋白表达研究维生素D对γ射线引起的BMSC损伤的保护作用。小鼠在6Gy的 γ 射线照射前48h每天皮下注射0.0625μg的1,25二羟基维生素D。结果发现，照射引起小鼠骨髓成纤维细胞集落形成单位（CFU-F）数显著减少。维生素D处理使照射后小鼠的CFU-F与对照组在同一水平。照射使BMSC凋亡率及其caspase-3蛋白的表达显著上升，而维生素D处理可以显著改善这种效果。这些资料说明维生素D具有保护照射引起的BMSC凋亡的作用。

Abstract

Radioprotective effect of vitamin D on bone marrow stroma1 cells (BMSCs) was studied. Mice were administrated subcutaneously with a daily dosage of 1,25 dihydrxyvitamin D for three days from 48h prior to 6.0Gy gamma-irradiation until they were sacrificed. The number of CFU-F, the apoptosis rate of bone marrow stromal cell, and the expressions of caspase-3 in BMSC were detected. The number of CFU-F significantly decreased after irradiation and vitamin D administration preserved the number of CFU-F in irradiated mice at same level as control. The apoptosis rate and relative level of caspase-3 in BMSC were significantly increased after irradiation and vitamin D administration ameliorated this effect. These data suggest that vitamin D can protect BMSC from apoptosis induced by irradiation.

关键词

γ射线骨髓基质细胞维生素D凋亡

Keywords

γ-IrradiationBone marrow stroma1 cellVitamin DApoptosis

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