Photochemical preparation of amino-functionalized magnetic nanogels for immobilization of streptavidin

GONG Peijun; HONG Jun; LIU Xingfen; YU Jiahui; FAN Chunhai; DONG Li; YAO Side

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Photochemical preparation of amino-functionalized magnetic nanogels for immobilization of streptavidin

更新时间：2023-03-30

- Photochemical preparation of amino-functionalized magnetic nanogels for immobilization of streptavidin
- Journal of Radiation Research and Radiation Processing Vol. 24, Issue 6, Pages: 345-350(2006)
- 作者机构：
  
  1.中国科学院上海应用物理研究所　上海　201800
  2.华东师范大学信息学院生物工程材料与器件研究所　上海　200062
  3.中国科学院研究生院　北京　100049
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GONG Peijun, HONG Jun, LIU Xingfen, et al. Photochemical preparation of amino-functionalized magnetic nanogels for immobilization of streptavidin. [J]. Journal of Radiation Research and Radiation Processing 24(6):345-350(2006)
DOI：

GONG Peijun, HONG Jun, LIU Xingfen, et al. Photochemical preparation of amino-functionalized magnetic nanogels for immobilization of streptavidin. [J]. Journal of Radiation Research and Radiation Processing 24(6):345-350(2006) DOI：

摘要

本工作提出制备胺基磁性纳米凝胶的一种改进方法。在含有烯丙基胺的Fe,3,O,4,水分散液中，运用紫外光辐照引发烯丙基胺原位聚合，一步法制备了聚烯丙基胺磁性纳米凝胶（Polyallylamine magnetic nanogels，PAA-MNGs）。透射电子显微镜（Transmission electron microscope，TEM）和光子相关光谱（Photo correlation spectroscope，PCS）表征了PAA-MNG的形貌和粒径分布；热重分析（Thermogravimetric analysis，TGA）确定了磁性成分的含量，振动样品磁强计（Vibrating sample magnetometer，VSM）测试表明其呈现超顺磁性。利用表面的伯胺基，PAA-MNGs可以方便地偶联蛋白等生物大分子。链亲和素（Streptavidin，SA）经1-乙基3-（3-二甲氨基）碳化二亚胺盐酸盐和N-羟基琥珀酰亚胺活化后可以共价固定在PAA-MNGs上，得到链亲和素包覆的纳米磁球（Streptavidin-coated magnetic nanoparticles，SA-MNPs）。使用探针生物素对硝基苯酯（Biotin p-nitrophenyl ester，BNPE）通过光谱检测证实了SA-MNPs的生物素结合能力。表明PAA-MNGs适合于作为载体偶联蛋白等生物大分子，并可能用于生物技术领域的诸多方面。

Abstract

An improved approach for preparing amino-modified magnetic nanogels has been developed in this paper. Polyallylamine magnetic nanogels (PAA-MNGs) were synthesized in one step by photoinitiation and ,in situ, photopolymerization of allylamine in Fe,3,O,4, aqueous suspension under UV irradiation. Morphology, mean diameter and diameter distribution of the PAA-MNGs were measured by TEM and PCS respectively, while the magnetite content was confirmed by TG and the superparamagnetism identified by VSM. Biomolecules, such as proteins, can be coupled to the surface of PAA-MNGs in virtue of amino groups. Streptavidin-coated magnetic nanoparticles (SA-MNPs) were prepared by covalent immobilization of streptavidin (SA) activated by 1-Ethyl-3- (3-dimethyllaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysuccinimid (NHS) on PAA-MNGs. The biotin binding capacity of SA-MNPs was proved by colorimetric measurements using the probe biotin p-nitrophenyl ester (BNPE). Therefore, PAA-MNGs may be a suitable vehicle to carry biomolecules for applications in many areas of biotechnology.

关键词

聚烯丙基胺磁性纳米凝胶紫外辐照链亲和素

Keywords

PolyallylamineMagnetic nanogelsUV irradiationStreptavidin

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