EFFECTS OF RADIATION ON IL-2Rα EXPRESSION OF SPLENOCYTES IN MICE

SU Xu; LUO Can; FU Shibo; LIU Jianxiang; JU Guizhi; LIU Shuzheng

您当前的位置：

首页 >

文章列表页 >

EFFECTS OF RADIATION ON IL-2Rα EXPRESSION OF SPLENOCYTES IN MICE

更新时间：2023-04-07

- EFFECTS OF RADIATION ON IL-2Rα EXPRESSION OF SPLENOCYTES IN MICE
- Journal of Radiation Research and Radiation Processing Vol. 17, Issue 1, Pages: 48-51(1999)
- 作者机构：
  
  1.白求恩医科大学卫生部放射生物学重点实验室　长春　130021
- 作者简介：
- 基金信息：
  
  the National Natural Science Foundation of China(39570188)
- DOI：
  CLC：
扫描看全文
SU Xu, LUO Can, FU Shibo, et al. EFFECTS OF RADIATION ON IL-2Rα EXPRESSION OF SPLENOCYTES IN MICE. [J]. Journal of Radiation Research and Radiation Processing 17(1):48-51(1999)
DOI：

SU Xu, LUO Can, FU Shibo, et al. EFFECTS OF RADIATION ON IL-2Rα EXPRESSION OF SPLENOCYTES IN MICE. [J]. Journal of Radiation Research and Radiation Processing 17(1):48-51(1999) DOI：

摘要

研究不同剂量、特别是低剂量辐射全身照射后，小鼠脾细胞IL-2Rα的变化规律，以揭示低剂量辐射免疫增强效应的发生机理。小鼠接受不同剂量X射线全身照射24h后，体外诱导脾细胞IL-2R的表达，采用直接免疫荧光流式细胞术检测脾细胞IL-2Rα表达的变化。结果 50～500mGy X射线全身照射后24h，小鼠脾细胞IL-2Rα表达显著增高,（p,<,0.05）,表现为CD25阳性细胞百分率明显高于假照射对照组，尤其以50mGy照射组表达最为明显（,p,<,0.01）。1.0～2.0Gy照射组，CD25阳性细胞百分率无明显变化,（p,>,0.05）。4.0～6.0 Gy全身照射后，CD25阳性细胞百分率明显降低（分别为,p,<,0.05,p,<,0.01）。以上结果提示：低剂量辐射可促进脾细胞IL-2R的表达，这对揭示低剂量辐射免疫增强效应的发生机理具有重要意义。较大剂量辐射全身照射的实验数据还表明：较大剂量辐射全身照射抑制脾细胞IL-2R的表达。

Abstract

To elucidate the possible mechanism of immunoenhancement after low dose radiation (LDR) . The regular of changes of IL-2Rα expression in splenocyte following low dose irradiation was studied. IL-2R expression was induced by Con A in vitro 24 hours after whole-body irradiation (WBI) . The expression of IL-2R in splenocyte was analyzed by direct immunofluorescence flow cytometry. It was found that the expression of IL-2R in splenocyte was shown significantly increased (p ,<,0.05) 24 hours after WBI with doses of 50~500mGy, in which the percentage of CD25 positive cells was significant higher than that in sham irradiated group, and the highest was in irradiated group with 50mGy (,p,<,0.01) . The percentage of CD25 positive cells did not change significantly after WBI with 1.0~2.0Gy (,p,>,0.05) . However, the percentage of CD25 positive cells decreased significantly after WBI with 4.0~6.0Gy (,p,<,0.05,p,<,0.01 respectively) . The results indicate that LDR might enhance and stimulate the expression of IL-2R in splenocyte. The results are important to elucidate the mechanism of immunoenhancement after low dose radiation. The results following WBI with higher doses suggested that WBI with higher doses might suppress the expression of IL-2R in splenocytes.

关键词

白细胞介素2受体电离辐射流式细胞术

Keywords

IL-2RIonizing radiationFlow cytometry

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Preparation of hydrogel-based photocatalytic composite materials by radiation technology

Clustered DNA damages induced by low-energy (1~20 eV) electrons and the general mechanism of transient anions

Research progress on the effect of water types on H₂ production in cement solidification treatment

Ionizing radiation promotes primary ciliogenesis in glioblastoma cells

Bioinformatic analysis of differentially expressed genes in tumor-associated fibroblasts after ionizing radiation

Related Author

No data

Related Institution

School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology

Hubei Key Laboratory of Radiation Chemistry and Functional Materials, Hubei University of Science and Technology

College of Pharmacy, Hubei University of Science and Technology

Xianning High-tech Hydrogel Dressing Industry Technology Research Institute

Research Institute of Photocatalysis, State Key Laboratory of Photocatalysis on Energy and Environment, College of Chemistry, Fuzhou University

⁰