APOPTOSIS OF OSTEOCLASTS INDUCED BY <sup>137</sup>Cs γ-IRRADIATION

LI Lingbo; GAO Jianjun; JIN Weifang; WANG Hongfu

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APOPTOSIS OF OSTEOCLASTS INDUCED BY ¹³⁷Cs γ-IRRADIATION

更新时间：2023-04-07

- APOPTOSIS OF OSTEOCLASTS INDUCED BY ¹³⁷Cs γ-IRRADIATION
- Journal of Radiation Research and Radiation Processing Vol. 17, Issue 1, Pages: 45-47(1999)
- 作者机构：
  
  1.上海医科大学放射医学研究所　上海　200032
- 作者简介：
- 基金信息：
  
  the "Ninth Five" Medical Science Faundation of China(96-906-05-05);Faundation of Shanghai Science Research
- DOI：
  CLC：
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LI Lingbo, GAO Jianjun, JIN Weifang, et al. APOPTOSIS OF OSTEOCLASTS INDUCED BY ¹³⁷Cs γ-IRRADIATION. [J]. Journal of Radiation Research and Radiation Processing 17(1):45-47(1999)
DOI：

LI Lingbo, GAO Jianjun, JIN Weifang, et al. APOPTOSIS OF OSTEOCLASTS INDUCED BY ¹³⁷Cs γ-IRRADIATION. [J]. Journal of Radiation Research and Radiation Processing 17(1):45-47(1999) DOI：

摘要

参照上海医科大学放射医学研究所七室建立的破骨细胞培养方法，将分离的破骨细胞培养在玻片上，利用0.5、1、3、5Gy ,137,Cs ,γ,射线照射破骨细胞，采用荧光染色方法观察辐射对破骨细胞形态的影响。结果表明：破骨细胞对辐射敏感，较低剂量即可引起破骨细胞凋亡，其中3Gy为诱导破骨细胞凋亡的最佳反应剂量，此时破骨细胞凋亡比例最高，可作为凋亡基因研究模型。为进一步探讨辐射所致骨质疾病的机理、预防、治疗提供实验依据。

Abstract

According to the method established previously, the osteoclasts were released and then settled on to glass slides. The numbers of apoptotic osteoclasts after in vitro γ-irradiation (0.5,1,3, 5 Gy) were measured by fluorescence staining assay. The results are that control samples and samples irradiated at 0.5 Gy could be distinguished in a statistically significant way. This suggests that osteoclasts are sensitive to irradiation and low doses can induce apoptosis. Furthermore, the percentage of apoptotic cells at 3 Gy reached the peak, this could be provided as a model for studying the molecular mechanism of apoptosis.

关键词

破骨细胞凋亡荧光染色辐射

Keywords

OsteoclastsApoptosisFluorescence staining assayRadiation

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APOPTOSIS OF OSTEOCLASTS INDUCED BY 137Cs γ-IRRADIATION

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APOPTOSIS OF OSTEOCLASTS INDUCED BY ¹³⁷Cs γ-IRRADIATION