EFFECTS OF INTERFERON-γ AND DEXAMETHASONE ON GROWTH OF NORMAL AND STIMULATED FIBROBLASTS BY 5-HYDROXYTRAPTAMINE

GAO Yabing; CUI Yufang; YANG Hong; XIONG Chengqi; XIA Guowei; WANG Dcwen

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EFFECTS OF INTERFERON-γ AND DEXAMETHASONE ON GROWTH OF NORMAL AND STIMULATED FIBROBLASTS BY 5-HYDROXYTRAPTAMINE

更新时间：2023-04-07

- EFFECTS OF INTERFERON-γ AND DEXAMETHASONE ON GROWTH OF NORMAL AND STIMULATED FIBROBLASTS BY 5-HYDROXYTRAPTAMINE
- Journal of Radiation Research and Radiation Processing Vol. 17, Issue 1, Pages: 36-40(1999)
- 作者机构：
  
  1.军事医学科学院放射医学研究所　北京　100850
- 作者简介：
- 基金信息：
  
  the foundation of the General Logistics Department of PLA
- DOI：
  CLC：
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GAO Yabing, CUI Yufang, YANG Hong, et al. EFFECTS OF INTERFERON-γ AND DEXAMETHASONE ON GROWTH OF NORMAL AND STIMULATED FIBROBLASTS BY 5-HYDROXYTRAPTAMINE. [J]. Journal of Radiation Research and Radiation Processing 17(1):36-40(1999)
DOI：

GAO Yabing, CUI Yufang, YANG Hong, et al. EFFECTS OF INTERFERON-γ AND DEXAMETHASONE ON GROWTH OF NORMAL AND STIMULATED FIBROBLASTS BY 5-HYDROXYTRAPTAMINE. [J]. Journal of Radiation Research and Radiation Processing 17(1):36-40(1999) DOI：

摘要

用MTT比色、AgNORs银染结合图像分析以及抗Ⅲ型胶原免疫组化技术观察并证实了γ-IFN和地塞米松（Dexa）对成纤维细胞生长的抑制作用。结果如下：（1）培养第3d,250×10,3,U/L的γ-IFN和100mg/L的Dexa对成纤维细胞生长的抑制率分别为43%和33%; （2）照后3、5d两种抑制剂对正常、受照射以及5-HT刺激的受照成纤维细胞增殖（OD值示之）均显示出明显的抑制作用；（3）250×10,3,U/L浓度的γ-IFN和150mg/L的Dexa对成纤维细胞AgNORs合成显示最强的抑制作用，其AgNORs颗粒数在5-HT组分别为对照值的58%和56%,而AgNORs3个定量参数的均值分别为对照值的51%和44%；（4）上述浓度的两种抑制剂对5-HT刺激的成纤维细胞Ⅲ型胶原合成也显示明显的抑制作用，在培养第3d Ⅲ型胶原阳性细胞数分别为对照值的62%和43%。

Abstract

Inhibitory effects of interferon-γ (γ-INF) and dexamethasone (Dexa) on growth of cultured fibroblasts were observed by MTT colorimetry, silver colloid technique of nucleolar of orgnizer regions associated protein (AgNORs) combined with image analysis and immunohistochemistry for type III collagen. The results showed that, (1) when γ-INF of 250×10,3,U/L and Dexa of 100mg/L were used, the inhibitory percentages of them on normal fibroblasts 3 days after culture were 43% and 33% respectively; (2) Above mentioned two inhibitors showed evident inhibitory effects on proliferation of normal, irradiated (on 3 or 5 days after irradiation) and 5-HT stimulated fibroblasts; (3) The third day after culture, the strongest inhibitory effects on fibroblasts AgNORs were observed when 250×10,3,U/L of γ-INF and 150mg/L of Dexa were delivered, the number of AgNORs granules in 5-HT stimulated fibroblasts decreased to 58% and 56% of normal control values; while the mean values of three quantitative parameters of AgNORs were also lower than those of control values; (4) The two inhibitors of above mentioned concentrations also demonstrated obvious restrained effect on the synthesis of type III collagen in 5-HT stimulated fibroblasts on day 3 after culture, the percentage of type III collagen positive fibroblasts were about 62% and 43% of control values.

关键词

γ干扰素地塞米松成纤维细胞5-羟色胺照射Ⅲ型胶原增殖抑制

Keywords

Interferon-γDexamethasoneFibroblasts5-HydroxytraptamineIrradiationType III collagenProliferation inhibition

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