G<sub>1</sub> ARREST AND THE EXPRESSIONS OF RELATIVE PROTEINS IN EL-4 CELLS INDUCED BY IONIZING RADIATION

JU Guizhi; FU Haiqing; LUO Can; FU Shibo

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G₁ ARREST AND THE EXPRESSIONS OF RELATIVE PROTEINS IN EL-4 CELLS INDUCED BY IONIZING RADIATION

更新时间：2023-04-07

- G₁ ARREST AND THE EXPRESSIONS OF RELATIVE PROTEINS IN EL-4 CELLS INDUCED BY IONIZING RADIATION
- Journal of Radiation Research and Radiation Processing Vol. 19, Issue 1, Pages: 67-70(2001)
- 作者机构：
  
  1.白求恩医科大学卫生部放射生物学重点实验　长春　130021
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JU Guizhi, FU Haiqing, LUO Can, et al. G₁ ARREST AND THE EXPRESSIONS OF RELATIVE PROTEINS IN EL-4 CELLS INDUCED BY IONIZING RADIATION. [J]. Journal of Radiation Research and Radiation Processing 19(1):67-70(2001)
DOI：

JU Guizhi, FU Haiqing, LUO Can, et al. G₁ ARREST AND THE EXPRESSIONS OF RELATIVE PROTEINS IN EL-4 CELLS INDUCED BY IONIZING RADIATION. [J]. Journal of Radiation Research and Radiation Processing 19(1):67-70(2001) DOI：

摘要

探讨X射线诱导EL-1细胞G,1,期阻滞及相关蛋白的表达。采用PI荧光标记，流式细胞术检测细胞周期的变化。用单克隆抗体免疫荧光标记，流式细胞术检测蛋白表达的变化。结果表明，2.0Gy和4.0Gy照射后12-72h, G,1,期EL-4细胞百分数显著高于假照射组（,p,<,0.05— ,p,<,0.001)。4.0Gy照射后EL-4细胞p53蛋白表达从照射后2h开始明显增高，持续至照射后24h（,p,<,0.05—,p,<,0.001）; p21蛋白表达在照射后2h开始明显增高，持续至照射后48h,（p,<, 0.05—,p,<,0.001）；GADD45蛋白表达在照射后2h开始明显增高，持续至照射后48h（,p,<,0.05—,p,<,0.001）；MDM2蛋白表达在照射后4h开始明显增高，持续至照射后24h,（p,<,0.05,—p,<, 0.001）。结果提示，中等剂量X射线照射可诱导EL-4细胞G,1,期阻滞。p53、p21和GADD45蛋白表达在电离辐射诱导EL-4细胞G,1,期阻滞中起重要作用。

Abstract

To investigate G,1, arrest and the expressions of relative proteins in EL-4 cells induced by ionizing radiation, flow cytometry (FCM) was used to analyze cell cycle through staining cells with propidium iodide (PI). And FCM was also employed to analyze protein expression of EL-4 cells stained wity monoclonal and polyclonal antibodise for their immunofluorescence. It was found that the number of EL-4 cells in G,1, phase increased significantly 12—72h after X-irradiation with doses of 2.0Gy and 4.0Gy. The resuits also showed significant increases of p53 protein expression at 2h to 24h (,p,<,0.05 —,p,<,0.001), p21 protein expression at 2h to 48h (,p,<,0.05—,p,<,0.001), GADD45 protein expression at 2h to 48h (,p,<,0.05 —,p,<, 0.001),and MDM2 protein expression at 4h to 24h (,p,<,0.05—,p,<,0.001) after 4.0Gy X-irradiation. These results suggest that G,1, arrest of EL-4 cells could be induced by ionizing radiation and the expression of p53, p21 and GADD45 proteins may play an important role in the mechanism.

关键词

电离辐射G1期阻滞蛋白表达

Keywords

Ionizing radiationG1 arrestProtein expression

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G1 ARREST AND THE EXPRESSIONS OF RELATIVE PROTEINS IN EL-4 CELLS INDUCED BY IONIZING RADIATION

DOI：

摘要

Abstract

关键词

Keywords

references

G₁ ARREST AND THE EXPRESSIONS OF RELATIVE PROTEINS IN EL-4 CELLS INDUCED BY IONIZING RADIATION