DIRECT OBSERVATION OF SITE—SELECTIVE DAMAGE OF DNA BY FLAVIN PHOTONUCLEASE

LU Changyuan; HAN Zhenhui; YAO Side; LIN Nianyun

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DIRECT OBSERVATION OF SITE—SELECTIVE DAMAGE OF DNA BY FLAVIN PHOTONUCLEASE

更新时间：2023-04-07

- DIRECT OBSERVATION OF SITE—SELECTIVE DAMAGE OF DNA BY FLAVIN PHOTONUCLEASE
- Journal of Radiation Research and Radiation Processing Vol. 19, Issue 1, Pages: 65-66(2001)
- 作者机构：
  
  1.中国科学院上海原子核研究所辐射化学开放实验室，上海　201800
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LU Changyuan, HAN Zhenhui, YAO Side, et al. DIRECT OBSERVATION OF SITE—SELECTIVE DAMAGE OF DNA BY FLAVIN PHOTONUCLEASE. [J]. Journal of Radiation Research and Radiation Processing 19(1):65-66(2001)
DOI：

LU Changyuan, HAN Zhenhui, YAO Side, et al. DIRECT OBSERVATION OF SITE—SELECTIVE DAMAGE OF DNA BY FLAVIN PHOTONUCLEASE. [J]. Journal of Radiation Research and Radiation Processing 19(1):65-66(2001) DOI：

摘要

近来研究人工合成能使DNA定位损伤的光核酸酶成为光医学、光化学和光生物学领域的研究热点,[1],。然而，目前光核酸酶对癌细胞DNA的作用机制尚不完全了解。尽管已公认DNA碱基阳离子自由基（或氧化性自由基）是核酸光敏氧化反应的最初、最重要的瞬态产物，然而至今未能直接观察到光氧化产生的碱基阳离子自由基的瞬态吸收光谱。这一问题已成为国际同行关注的难题。因为它是激光束进入细胞时激发生物大分子动态过程的关键，是阐明电荷转移光氧化治疗畸变基因、调控基因表达机制的原初证据。现有的研究主要集中在稳态分析上,[1.2],，尽管也有少数关于瞬态方面的文献报道，但主要以光敏化剂自身的性质研究为主,[1,3],。在最近的实验中我们发现，光核酸酶经光激发产生的敏化剂活性粒子无论是激发态还是氧化性自由基主要与研究的DNA发生反应，而与识别系统中的寡聚核苷酸不起作用。即连接在光敏化剂上的寡聚核苷酸主要起定位作用。从而将本实验室及其它国际同类实验室单凭光敏化剂与DNA及其组分的敏化作用来推导光核酸酶的作用机理推进了一步。

Abstract

The interaction of oxidized FAD radical with dGMP, dAMP and single—stranded DNA (ssDNA) was investigated in neutral aqueous solution using time—resolved 248 nm laser flash photolysis aimed at elucidation of the initial photosensitization mechanism. The characterized absorption spectra of transient species were observed. Moreover,direct observation of stabilized DNA guanyl radical has provided transient evidence for site—selective photosensitized damage of DNA at guanine moiety, which has simulated the major initial species produced by the direct effect of ionizing radiation or UV light on DNA.

关键词

DNA黄素腺嘌呤二核苷酸（FAD）激光光解鸟嘌呤阳离子自由基定位损伤

Keywords

DNAFlavin adenine dinucleotide (FAD)Laser flash photolysisGuanyl radicalSite—selective damage

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