INFLUENCES OF IONIZING RADIATION ON APOPTOSIS AND CELL CYCLE OF HelaS<sub>3</sub> CELL LINES

JIN Guanghui; XUE Lixiang; FU Shibo; SU Xu

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INFLUENCES OF IONIZING RADIATION ON APOPTOSIS AND CELL CYCLE OF HelaS₃ CELL LINES

更新时间：2023-04-07

- INFLUENCES OF IONIZING RADIATION ON APOPTOSIS AND CELL CYCLE OF HelaS₃ CELL LINES
- Journal of Radiation Research and Radiation Processing Vol. 19, Issue 3, Pages: 177-180(2001)
- 作者机构：
  
  1.白求恩医科大学卫生部放射生物学重点实验室　长春　130021
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JIN Guanghui, XUE Lixiang, FU Shibo, et al. INFLUENCES OF IONIZING RADIATION ON APOPTOSIS AND CELL CYCLE OF HelaS₃ CELL LINES. [J]. Journal of Radiation Research and Radiation Processing 19(3):177-180(2001)
DOI：

JIN Guanghui, XUE Lixiang, FU Shibo, et al. INFLUENCES OF IONIZING RADIATION ON APOPTOSIS AND CELL CYCLE OF HelaS₃ CELL LINES. [J]. Journal of Radiation Research and Radiation Processing 19(3):177-180(2001) DOI：

摘要

研究了电离辐射对HelaS,3,细胞凋亡和细胞周期的影响，进一步探讨细胞凋亡与细胞周期的关系，为肿瘤放化疗方案的制定提供基础资料。采用PI、Hoechst33342双染和PI单染，用流式细胞术检测细胞凋亡和细胞周期。结果表明，给予0.5—4.0Gy X射线照射后8h细胞凋亡开始增加，12h达最大值，12—24h有下降趋势，但24h开始又逐渐增加，到72h时又接近12h水平。S期和G,2,/M期细胞均明显增加，并呈剂量依赖性，G,0,/G,1,期细胞则呈剂量依赖性减少，且这种变化在受照射后24h最明显。说明一定剂量电离辐射可以诱导明显的G,2,/M阻滞和S期阻滞，并可诱导HelaS,3,细胞凋亡的产生，两者之间有一定的关系。

Abstract

Changes of apoptosis and cell cycle progression of HeLaS,3, cells following irradiation with different doses of X rays were observed. The percentages of apoptotic cells were examined by flow cytometry (FCM) after the cells had been stained with propidium iodine (PI) and Hoechst33342(HO),and the cell cycle was detected by FCM stained with only PI. It was found that the percentages of apoptotic cells and the cells in S phase and G,2,/M phase increased after irradiation with 0.5—4.0Gy X rays. So irradiation may induce apoptosis and cell cycle block for HelaS,3, cell lines.

关键词

电离辐射细胞凋亡细胞周期HelaS3

Keywords

Ionizing radiationApoptosisCell cycleHelaS3

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INFLUENCES OF IONIZING RADIATION ON APOPTOSIS AND CELL CYCLE OF HelaS3 CELL LINES

DOI：

摘要

Abstract

关键词

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INFLUENCES OF IONIZING RADIATION ON APOPTOSIS AND CELL CYCLE OF HelaS₃ CELL LINES