EFFECTS OF WHOLE BODY IRRADIATION WITH X RAYS ON CYCLIN B<sub>1</sub> AND CDC2 TRANSCRIPTION LEVEL OF SPLENOCYTES IN MICE

YE Fei; LIU Shuzheng

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EFFECTS OF WHOLE BODY IRRADIATION WITH X RAYS ON CYCLIN B₁ AND CDC2 TRANSCRIPTION LEVEL OF SPLENOCYTES IN MICE

更新时间：2023-04-07

- EFFECTS OF WHOLE BODY IRRADIATION WITH X RAYS ON CYCLIN B₁ AND CDC2 TRANSCRIPTION LEVEL OF SPLENOCYTES IN MICE
- Journal of Radiation Research and Radiation Processing Vol. 19, Issue 3, Pages: 181-185(2001)
- 作者机构：
  
  1.白求恩医科大学卫生部放射生物学重点实验室　长春　130021
- 作者简介：
- 基金信息：
  
  the National Natural Science Foundation of China(39570188)
- DOI：
  CLC：
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YE Fei, LIU Shuzheng. EFFECTS OF WHOLE BODY IRRADIATION WITH X RAYS ON CYCLIN B₁ AND CDC2 TRANSCRIPTION LEVEL OF SPLENOCYTES IN MICE. [J]. Journal of Radiation Research and Radiation Processing 19(3):181-185(2001)
DOI：

YE Fei, LIU Shuzheng. EFFECTS OF WHOLE BODY IRRADIATION WITH X RAYS ON CYCLIN B₁ AND CDC2 TRANSCRIPTION LEVEL OF SPLENOCYTES IN MICE. [J]. Journal of Radiation Research and Radiation Processing 19(3):181-185(2001) DOI：

摘要

采用Northern blot杂交法研究了低、高剂量X射线全身照射后不同时间小鼠脾细胞cyclin B,1,和cdc2基因转录水平的变化。结果表明，75mGy X射线全身照射后2h及12—24h小鼠脾细胞cydin B,1, mRNA表达量略有升高，分别为假照组的1.25、1.27和1.22倍，48h回降至假照水平；而2.0Gy照射后4h开始下降，12h降至最低，与假照组相比降低了39%,48h恢复至假照组水平。同时观察了cd,c,2 mRNA表达量的变化，结果表明，与假照组相比75mGy X射线全身照射后2—48h的各时间点小鼠脾细胞cdc2 mRNA表达量未见明显变化；而2.0Gy照射后的时程变化与相同剂量照射后cyclin B,1,的变化基本一致。结果提示：低剂量辐射可诱导小鼠脾细胞cyclin B,1,转录水平增高，进而促进其细胞周期进程，但对cdc2转录水平无影响；相反，较高剂量辐射可诱导cyclin B,1,和cdc2转录水平均明显降低，最终发生G,2,期阻滞。

Abstract

The changes in cyclin B,1, and cdc2 transcription level of splenocytes in mice were observed after WBI with low and high doses of Xrays. The results showed that cyclin B,1, mRNA expression slightly increased at 2h and 12—24h after 75mGy X rays, being 1.25, 1.27 and 1.22 times of sham—irradiated group, returning to sham—irradiated level at 48h. However, cyclin B,1, mRNA began to decrease at 4h after irradiation with 2.0Gy X rays,reaching its lowest level at 12h, with a decrease of 39%, and returning to the level of sham—irradiated control. In addition, we observed the time course of cdc2 mRNA expression. The results showed that there were no changes in cdc2 mRNA expression at 2—48h after 75mGy WBI, but the time course of cdc2 mRNA expression with 2.0Gy WBI was the same as cyclin B,1, after the same dose. The results indicated that low dose irradiation could induce an increase in cyclin B,1, transcription level, accelerating the progression of cell cycle, but did not affect the expression of cdc2 gene. In contrast,high dose irradiation could induce the decrease of above two factors,with marked G,2, arrest

关键词

X射线脾细胞cyclin B1cdc2转录水平

Keywords

X raysSplenocytesCyclin B1cdc2 transcription level

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EFFECTS OF WHOLE BODY IRRADIATION WITH X RAYS ON CYCLIN B1 AND CDC2 TRANSCRIPTION LEVEL OF SPLENOCYTES IN MICE

DOI：

摘要

Abstract

关键词

Keywords

references

EFFECTS OF WHOLE BODY IRRADIATION WITH X RAYS ON CYCLIN B₁ AND CDC2 TRANSCRIPTION LEVEL OF SPLENOCYTES IN MICE