EFFECT OF IONIZING RADIATION ON THE CDK4 EXPRESSION IN MOUSE BONE MARROW CELLS

MA Shumei; JU Guizhi

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EFFECT OF IONIZING RADIATION ON THE CDK4 EXPRESSION IN MOUSE BONE MARROW CELLS

更新时间：2023-04-07

- EFFECT OF IONIZING RADIATION ON THE CDK4 EXPRESSION IN MOUSE BONE MARROW CELLS
- Journal of Radiation Research and Radiation Processing Vol. 19, Issue 3, Pages: 191-194(2001)
- 作者机构：
  
  1.白求恩医科大学卫生部放射生物学重点实验室　长春　130021
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MA Shumei, JU Guizhi. EFFECT OF IONIZING RADIATION ON THE CDK4 EXPRESSION IN MOUSE BONE MARROW CELLS. [J]. Journal of Radiation Research and Radiation Processing 19(3):191-194(2001)
DOI：

MA Shumei, JU Guizhi. EFFECT OF IONIZING RADIATION ON THE CDK4 EXPRESSION IN MOUSE BONE MARROW CELLS. [J]. Journal of Radiation Research and Radiation Processing 19(3):191-194(2001) DOI：

摘要

采用流式细胞术和Northern blot检测X射线全身照射后小鼠骨髓细胞CDK4转录水平和蛋白表达的变化。结果表明，2 Gy X射线全身照射后4—48h CDK4 mRNA转录水平及蛋白表达均明显下降；0.5—6 Gy X射线全身照射后12 h CDK4 mRNA水平及蛋白表达亦呈降低改变。结果提示，CDK4在电离辐射诱导的小鼠骨髓细胞G,1,期阻滞中可能起重要作用。

Abstract

To investigate the effect of ionizing radiation on the CDK4 expression in mouse bone marrow cells, Flow cytometry (FCM) and Northern blot were used to analyze the changes in the level of CDK4 mRNA and protein expression respectively after whole body irradiation(WBI) with X rays. The results indicated that both the levels of CDK4 mRNA and protein expression decreased significantly from 2h to 72h after 2Gy X rays. Meanwhile, we found a dose—dependent relationship for these two levels at 12h after irradiation with different doses (0.5,1, 2,4,6Gy). These results suggested that the effect of ionizing irradiation on CDK4 expression maybe have profound significance and desire further research.

关键词

CDK4电离辐射骨髓细胞G1期阻滞

Keywords

CDK4Ionizing radiationBone marrow cellsG1 arrest

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