USE OF SINGLE CELL MICROGEL ELECTROPHORESIS TO DETECT THE CELLUAR RADIOSENSITIVITY OF TWO FIBROBLAST STRAINS

XU Yongzhong; ZHENG Siying; ZHU Wei; ZHAO Jingyong; CAO Jianping

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USE OF SINGLE CELL MICROGEL ELECTROPHORESIS TO DETECT THE CELLUAR RADIOSENSITIVITY OF TWO FIBROBLAST STRAINS

更新时间：2023-04-07

- USE OF SINGLE CELL MICROGEL ELECTROPHORESIS TO DETECT THE CELLUAR RADIOSENSITIVITY OF TWO FIBROBLAST STRAINS
- Journal of Radiation Research and Radiation Processing Vol. 19, Issue 3, Pages: 186-190(2001)
- 作者机构：
  
  1.苏州大学核医学院放射医学系　苏州　215007
  2.复旦大学遗传所　上海　200433
- 作者简介：
- 基金信息：
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XU Yongzhong, ZHENG Siying, ZHU Wei, et al. USE OF SINGLE CELL MICROGEL ELECTROPHORESIS TO DETECT THE CELLUAR RADIOSENSITIVITY OF TWO FIBROBLAST STRAINS. [J]. Journal of Radiation Research and Radiation Processing 19(3):186-190(2001)
DOI：

XU Yongzhong, ZHENG Siying, ZHU Wei, et al. USE OF SINGLE CELL MICROGEL ELECTROPHORESIS TO DETECT THE CELLUAR RADIOSENSITIVITY OF TWO FIBROBLAST STRAINS. [J]. Journal of Radiation Research and Radiation Processing 19(3):186-190(2001) DOI：

摘要

应用单细胞微凝胶电泳（SCGE）技术以及噻唑蓝（MTT）比色法检测辐射诱发的成纤维细胞系AT5BIVA和GM637的初始DNA双链断裂数及断裂后的修复与细胞放射敏感性之间的关系。结果表明，AT5BIVA细胞系的放射敏感性显著高于GM637。两种细胞系的初始DNA双链断裂数均随剂量的增加而增加，呈显著的剂量效应关系，在给定的剂量点，辐射诱发的AT5BIVA细胞系的初始DNA双链断裂数显著高于GM637。AT5BIVA细胞系对辐射诱发的DNA双链断裂的修复能力小于GM637。结果提示，辐射诱发的初始DNA双链断裂数及断裂后的修复与细胞的放射敏感性均有一定的相关性，作为细胞放射敏感性预测指标具有很大的应用潜势。

Abstract

Single cell microgel electrophoresis(SCGE) and MTT assay were used to determine the relationships between radiation—induced initial DNA double—strand breaks(DSBs), the ability of cells to repair DSBs and cellular radiosensitivity in both AT5BIVA and GM637 cell lines. The results demonstrated that AT5BIVA cell line was significantly more radiosensitive than GM637. The initial DSBs in both AT5BIVA and GM637 lines increased with the rise of irradiation dose,and showed significant dependence on dose. At a given dose of 20Gy, radiation—induced initial DSBs in AT5BIVA cell line was signficantly higher than those in GM637. The ability of AT5BIVA cell line to repair DSBs was more powerful than that of GM637. The results suggested that the radiation—induced initial DSBs in cells and the ability of cells to repair DSBs both correlated with cellular radiosensitivity to a certain extent, and could be used as potential predictors of intrinsic radiosensitivity of cells.

关键词

单细胞微凝胶电泳DNA双链断裂修复放射敏感性

Keywords

Single cell microgel electrophoresisDNA double—strand breaksRepairRadiosensitivity

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Related Institution

Department of Radiation Oncology, The First Affiliate Hospital of Anhui University of Science and Technology

Department of Cell Biology, School of Medicine, Anhui University of Science and Technology

First Clinical Hospital, School of Medicine, Anhui University of Science and Technology

Department of Radiation Oncology, the Fourth Affiliated Hospital of China Medical University

苏州大学医学部放射医学与防护学院/放射医学及交叉学科研究院

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