STUDIES ON INCREASE OF INTRACELLULAR REACTIVE OXYGEN SPECIES AND OXIDATIVE DNA DAMAGE IN<sup>60</sup>Co <italic style="font-style: italic">γ</italic> RAY IRRADIATED BEP2D CELLS

ZHU Maoxiang; YANG Zhihua; GONG Yifen; LU Ying; CAO Zhenshan

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STUDIES ON INCREASE OF INTRACELLULAR REACTIVE OXYGEN SPECIES AND OXIDATIVE DNA DAMAGE IN⁶⁰Co γ RAY IRRADIATED BEP2D CELLS

更新时间：2023-04-07

- STUDIES ON INCREASE OF INTRACELLULAR REACTIVE OXYGEN SPECIES AND OXIDATIVE DNA DAMAGE IN⁶⁰Co γ RAY IRRADIATED BEP2D CELLS
- Journal of Radiation Research and Radiation Processing Vol. 19, Issue 4, Pages: 270-274(2001)
- 作者机构：
  
  1.军事医学科学院放射医学研究所　北京　100850
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ZHU Maoxiang, YANG Zhihua, GONG Yifen, et al. STUDIES ON INCREASE OF INTRACELLULAR REACTIVE OXYGEN SPECIES AND OXIDATIVE DNA DAMAGE IN⁶⁰Co γ RAY IRRADIATED BEP2D CELLS. [J]. Journal of Radiation Research and Radiation Processing 19(4):270-274(2001)
DOI：

ZHU Maoxiang, YANG Zhihua, GONG Yifen, et al. STUDIES ON INCREASE OF INTRACELLULAR REACTIVE OXYGEN SPECIES AND OXIDATIVE DNA DAMAGE IN⁶⁰Co γ RAY IRRADIATED BEP2D CELLS. [J]. Journal of Radiation Research and Radiation Processing 19(4):270-274(2001) DOI：

摘要

用,60,Co γ射线照射人支气管上皮细胞（BEP2D）。细胞内过氧化氢（H,2,O,2,）和超氧阴离子（O,2,•—,）分别用分子探针2',7'—二氯荧光黄双乙酸盐（DCFH—DA）和氢化乙锭（HE）进行标记，并通过流式细胞仪测定荧光产物2',7'—二氯荧光黄（DCF）和溴乙锭（EB）荧光强度进行相对定量分析。DNA氧化损伤产物8—羟基脱氧鸟嘌呤（OH8dG）含量用高压液相色谱结合电化学方法（HPLC—ECD）测定。结果表明，,60,Co γ射线诱发BEP2D细胞内活性氧（ROS）水平和OH8dG含量均显著增高，并有良好的剂量效应关系。进一步分析显示，,60,Co γ射线照射诱发BEP2D细胞内ROS增高与DNA氧化损伤产物OH8dG含量呈明显正相关，提示辐射对细胞的损伤效应可能与其诱发细胞内ROS增高及其对DNA氧化损伤机制有关。

Abstract

HPV—16 immortalized human bronchial epithelial cells (BEP2D) were irradiated by ,60,Co gamma rays. 2', 7'—dichlorofluorescein and ethidium bromide, fluorescent products of the membrane—permeable dyes 2', 7'—dichloroflurescin diacetate and hydroethine, respectively,were used to monitor the intracellular production of hydrogen peroxides (H,2,O,2,) and superoxide anions (O,2,•-,) respectively, by flow cytometry. 8—hydroxydeoxygunosine (OH8dG), a production of oxidative DNA damage, was examined with HPLC—ECD from extracted DNA. The results showed that the ROS productions and DNA adduct OH8dG in ,60,Co gamma rays irradiated BEP2D cells increased remarkably,and revealed better dose—response correlation. Further analysis indicated the positive correlation between intracellular ROS and content of OH8dG induced by ,60,Co gamma rays. Therefore, the effects of radiation on cell were involved increase of intracellular ROS and its production of oxidative DNA damage.

关键词

60Co γ射线照射人支气管上皮细胞活性氧DNA氧化损伤8—羟基脱氧鸟嘌呤

Keywords

60Co gamma rays irradiationBEP2DROSOxidative DNA damage8—hydroxydeoxygunosine.

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STUDIES ON INCREASE OF INTRACELLULAR REACTIVE OXYGEN SPECIES AND OXIDATIVE DNA DAMAGE IN60Co γ RAY IRRADIATED BEP2D CELLS

DOI：

摘要

Abstract

关键词

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references

STUDIES ON INCREASE OF INTRACELLULAR REACTIVE OXYGEN SPECIES AND OXIDATIVE DNA DAMAGE IN⁶⁰Co γ RAY IRRADIATED BEP2D CELLS