EFFECT OF IONIZING RADIATION ON EXPRESSIONS OF p16、CyclinD1 and CDK4 IN MOUSE THYMOCYCTES

WANG Xiaomei; JU Guizhi; MA Shumei; FU Shibo

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EFFECT OF IONIZING RADIATION ON EXPRESSIONS OF p16、CyclinD1 and CDK4 IN MOUSE THYMOCYCTES

更新时间：2023-04-07

- EFFECT OF IONIZING RADIATION ON EXPRESSIONS OF p16、CyclinD1 and CDK4 IN MOUSE THYMOCYCTES
- Journal of Radiation Research and Radiation Processing Vol. 19, Issue 4, Pages: 275-278(2001)
- 作者机构：
  
  1.白求恩医科大学卫生部放射生物学重点实验室　长春　130021
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WANG Xiaomei, JU Guizhi, MA Shumei, et al. EFFECT OF IONIZING RADIATION ON EXPRESSIONS OF p16、CyclinD1 and CDK4 IN MOUSE THYMOCYCTES. [J]. Journal of Radiation Research and Radiation Processing 19(4):275-278(2001)
DOI：

WANG Xiaomei, JU Guizhi, MA Shumei, et al. EFFECT OF IONIZING RADIATION ON EXPRESSIONS OF p16、CyclinD1 and CDK4 IN MOUSE THYMOCYCTES. [J]. Journal of Radiation Research and Radiation Processing 19(4):275-278(2001) DOI：

摘要

采用流式细胞术（FCM）检测了不同剂量X射线全身照射后，昆明种小鼠胸腺细胞p16、CyclinD1、CDK4蛋白表达的时程变化和量效关系。结果表明，2Gy X射线照射后8h小鼠胸腺细胞p16表达明显增高，24h达到峰值，持续至照射后48h, 72h降至正常水平；CDK4表达水平在照射后8h明显降低，12h降至最低，照射后48h恢复近正常水平；周期蛋白CyclinD1表达轻度下调。不同剂量（0.5—4Gy）照射后p16蛋白表达随着剂量的增加而增加，2Gy组与假照射组相比显著增多（,p,<,0.01) ;CDK4蛋白表达在2Gy照射后明显低于假照射组（,p<,0,.,01）; CyclinD1表达在0.5—2Gy照射后可见下降趋势。结果提示：p16/ CyclinD1/ CDK4/pRb通路在电离辐射诱导胸腺细胞G,1,期阻滞中可能起着十分重要的作用。

Abstract

Using flow cytometry (FCM), The changes in expressions of p16, CyclinD1 and CDK4 in the thymocytes of mice treated with different doses of whole—body irradiation or with a fixed dose and different post—irradiation intervals were analyzed. The results showed that the expression of p16 protein in mouse thymocytes began to increase significantly at 8h after 2Gy X irradiation, reached the peak level at 24h, kept a high level until 48h, and recovered at 72h. The CDK 4 protein expression decreased at 8h after 2Gy irradiation, reached the bottom level at 12h, and recovered at 48h. The expression of CyclinD1 protein showed a little reduction only after 2Gy irradiation. Overexpression of p16 depended on the doses of radiation (0.5—4Gy). The expression of p16 in 2Gy group was significantly increased in comparison with that in the sham—irradiated group (,p,<,0.01). The reduction of CDK4 expression was significant in 2Gy group (,p,<,0.01). CyclinD1 expression showed its decrease after 0.5—2Gy irradiation. These results suggested that p16/CyclinD1/CDK4/pRb pathway might play an important role in G,1, arrest of thymocytes induced by ionizing radiation.

关键词

电离辐射p16CDK4CyclinD1

Keywords

Ionizing radiationp16CDK4CyclinD1

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