INFLUENCES ON TIME COURSE OF APOPTOSIS OF HeLaS<sub>3</sub> CELLS AFTER IRRADIATION WITH DIFFERENT DOSES X RAYS

JIN Guanghui; XUE Lixiang; FU Shibo; SU Xu

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INFLUENCES ON TIME COURSE OF APOPTOSIS OF HeLaS₃ CELLS AFTER IRRADIATION WITH DIFFERENT DOSES X RAYS

更新时间：2023-04-07

- INFLUENCES ON TIME COURSE OF APOPTOSIS OF HeLaS₃ CELLS AFTER IRRADIATION WITH DIFFERENT DOSES X RAYS
- Journal of Radiation Research and Radiation Processing Vol. 19, Issue 4, Pages: 307-310(2001)
- 作者机构：
  
  1.白求恩医科大学卫生部放射生物重点实验室　长春　130021
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JIN Guanghui, XUE Lixiang, FU Shibo, et al. INFLUENCES ON TIME COURSE OF APOPTOSIS OF HeLaS₃ CELLS AFTER IRRADIATION WITH DIFFERENT DOSES X RAYS. [J]. Journal of Radiation Research and Radiation Processing 19(4):307-310(2001)
DOI：

JIN Guanghui, XUE Lixiang, FU Shibo, et al. INFLUENCES ON TIME COURSE OF APOPTOSIS OF HeLaS₃ CELLS AFTER IRRADIATION WITH DIFFERENT DOSES X RAYS. [J]. Journal of Radiation Research and Radiation Processing 19(4):307-310(2001) DOI：

摘要

采用PI和Hoechst33342双染、流式细胞术（FCM）和图像分析术（ICM）检测了不同剂量电离辐射对HeLaS,3,细胞凋亡的影响。结果表明，HeLaS,3,细胞在分别受到0.5—4.0Gy X射线照射后8h，其细胞凋亡呈增高趋势，但无统计学意义。照射后12h，细胞凋亡显著增加（p,<,0.01）,并达峰值。随后，细胞凋亡率迅速下降，48h,又有所回升。且流式细胞术与图像分析术所得结果数据相近，趋势一致。

Abstract

Changes of time course in apoptosis of HeLaS,3, cells following irradiation with different doses X rays were observed. The percentages of apoptotic cells were examined by both flow cytometry(FCM) and image cytometry (ICM) after the cells were stained by propidium iodine (PI) and Hoechst33342 (HO). It was founded that the percentages of apoptotic cells increased after irradiation with 0.5—4.0Gy X rays and the peak value emerged at the 12th hour after irradiation. In additon the values examined by both FCM and ICM were nearly same and the tendency was identical. So irradiation may induce apoptosis of HeLaS,3, cell lines.

关键词

电离辐射细胞凋亡流式细胞术图像分析术

Keywords

IrradiationApoptosisFlow cytometryImage cytometry

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Teaching and Research Department of Human Anatomy and Histology and Embryology, Medical Department of Qingdao University

Medical Department of Qingdao University

School of Pharmacy, Lanzhou University

Key Laboratory of Space Radiobiology of Gansu Province, Institute of Modern Physics, Chinese Academy of Science

University of Chinese Academy of Sciences

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INFLUENCES ON TIME COURSE OF APOPTOSIS OF HeLaS3 CELLS AFTER IRRADIATION WITH DIFFERENT DOSES X RAYS

DOI：

摘要

Abstract

关键词

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references

INFLUENCES ON TIME COURSE OF APOPTOSIS OF HeLaS₃ CELLS AFTER IRRADIATION WITH DIFFERENT DOSES X RAYS