STUDIES OF DNA DAMAGE IN TUMOR CELLS INDUCED BY RADIATION EXPOSURE BY MEANS OF SINGLE CELL GEL ELECTROPHORESIS

LIU Fenju; WANG Tao; HONG Chengjiao; XU Yujie; SU Liaoyuan; LIU Yongbiao

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STUDIES OF DNA DAMAGE IN TUMOR CELLS INDUCED BY RADIATION EXPOSURE BY MEANS OF SINGLE CELL GEL ELECTROPHORESIS

更新时间：2023-04-07

- STUDIES OF DNA DAMAGE IN TUMOR CELLS INDUCED BY RADIATION EXPOSURE BY MEANS OF SINGLE CELL GEL ELECTROPHORESIS
- Journal of Radiation Research and Radiation Processing Vol. 19, Issue 4, Pages: 302-306(2001)
- 作者机构：
  
  1.苏州大学核医学院　苏州　215007
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LIU Fenju, WANG Tao, HONG Chengjiao, et al. STUDIES OF DNA DAMAGE IN TUMOR CELLS INDUCED BY RADIATION EXPOSURE BY MEANS OF SINGLE CELL GEL ELECTROPHORESIS. [J]. Journal of Radiation Research and Radiation Processing 19(4):302-306(2001)
DOI：

LIU Fenju, WANG Tao, HONG Chengjiao, et al. STUDIES OF DNA DAMAGE IN TUMOR CELLS INDUCED BY RADIATION EXPOSURE BY MEANS OF SINGLE CELL GEL ELECTROPHORESIS. [J]. Journal of Radiation Research and Radiation Processing 19(4):302-306(2001) DOI：

摘要

为探测肿瘤细胞的辐射敏感性，应用单细胞凝胶电泳技术和,3,H—TdR掺入方法，对,γ,射线照射的K562、SMMC—772和HOS8603细胞株单细胞DNA链断裂的辐射效应进行了研究，实验表明上述3种肿瘤细胞在受到1—20Gy ,60,Co ,γ,射线照射后细胞迁移率增高，迁移距离延长，并且DNA合成受到抑制，,3,H—TdR掺入下降。

Abstract

In order to detect the radiation sensitivity of tumor cells,the methods of single cell gel electrophoresis (SCGE) and ,3,H—TdR incorporation were applieded to determine effects of radiation on single strand breaks of DNA in K562、SMMC—772 and HOS8603 cell lines. The experimental results proved that the cell migration distance was prolonged,DNA syntheses was restricted and the ,3,H—TdR incorporation rate was decreased in the above—mentioned 3 tumor cells lines after irradiation by ,60,Co ,γ, rays(1—20Gy).

关键词

细胞凝胶电泳DNA损伤肝癌细胞（SMMC772）骨肉瘤细胞（HOS8603）人红白血病细胞（K562）

Keywords

Single cell gel electrophoresis (SCGE)DNA damage3H—TdR incorporationSMMC—772HOS8603K562 cell lines

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