DETECTING DNA DOUBLE STRAND BREAKS AFTER INONIZING IRRADIATION BY CONSTANT-FILED GEL ELECTROPHORESIS

Li Yu; Zheng Xiulong; Luo Chengji; Cai Jianming; Meng Xiangshun; Wu Wei; Gao Jianguo

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DETECTING DNA DOUBLE STRAND BREAKS AFTER INONIZING IRRADIATION BY CONSTANT-FILED GEL ELECTROPHORESIS

更新时间：2023-04-18

- DETECTING DNA DOUBLE STRAND BREAKS AFTER INONIZING IRRADIATION BY CONSTANT-FILED GEL ELECTROPHORESIS
- Journal of Radiation Research and Radiation Processing Vol. 16, Issue 4, Pages: 234-237(1998)
- 作者机构：
  
  1.第二军医大学放射医学研究所　上海　200433
  (1).第三军医大学防原教研室　重庆　630038
- 作者简介：
- 基金信息：
  
  The National Foundation of China
- DOI：
  CLC：
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Li Yu, Zheng Xiulong, Luo Chengji, et al. DETECTING DNA DOUBLE STRAND BREAKS AFTER INONIZING IRRADIATION BY CONSTANT-FILED GEL ELECTROPHORESIS. [J]. Journal of Radiation Research and Radiation Processing 16(4):234-237(1998)
DOI：

Li Yu, Zheng Xiulong, Luo Chengji, et al. DETECTING DNA DOUBLE STRAND BREAKS AFTER INONIZING IRRADIATION BY CONSTANT-FILED GEL ELECTROPHORESIS. [J]. Journal of Radiation Research and Radiation Processing 16(4):234-237(1998) DOI：

摘要

应用普通恒场琼脂糖凝胶电泳检测受,γ,射线照射后小鼠胸腺细胞DNA双链断裂（dsb）。在一定条件下，该法可以获得与脉冲凝胶电泳相似的结果。用此法观察了离体照射小鼠胸腺细胞DNAdsb修复作用，发现该法可以检测到DNA“梯状”带，可做为一种研究细胞凋亡的新方法。

Abstract

It was observed that constant-field gel electrophoresis can detect DNA double breaks (dsb) by irradiation. The dsb degree of mice thymocyte by 0~50 Gy (irradiation and the dsb repair in two hours after 30Gy irradiation was studied,the "DNA ladder" was also found using the method.

关键词

恒场琼脂糖凝胶电泳γ射线照射DNA双链断裂（dsb）

Keywords

Constant-field gel electrophoresesIrradiationDNA double strand breaks (dsb)

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