COMPARISON OF TRANSFORMATION, DNA SINGLE STRAND BREAKS AND ITS REPAIR IN HUMAN BLOOD LYMPHOCYTES STIMULATED WITH PHA, ConA AND PWM FOLLOWING EXPOSURE TO γ-RAYS

Geng Yongzhi; Su Liaoyuan; Liu Keliang

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COMPARISON OF TRANSFORMATION, DNA SINGLE STRAND BREAKS AND ITS REPAIR IN HUMAN BLOOD LYMPHOCYTES STIMULATED WITH PHA, ConA AND PWM FOLLOWING EXPOSURE TO γ-RAYS

更新时间：2023-04-23

- COMPARISON OF TRANSFORMATION, DNA SINGLE STRAND BREAKS AND ITS REPAIR IN HUMAN BLOOD LYMPHOCYTES STIMULATED WITH PHA, ConA AND PWM FOLLOWING EXPOSURE TO γ-RAYS
- Journal of Radiation Research and Radiation Processing Vol. 7, Issue 4, Pages: 30-35(1989)
- 作者机构：
  
  1.苏州医学院，苏州
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Geng Yongzhi, Su Liaoyuan, Liu Keliang. COMPARISON OF TRANSFORMATION, DNA SINGLE STRAND BREAKS AND ITS REPAIR IN HUMAN BLOOD LYMPHOCYTES STIMULATED WITH PHA, ConA AND PWM FOLLOWING EXPOSURE TO γ-RAYS. [J]. Journal of Radiation Research and Radiation Processing 7(4):30-35(1989)
DOI：

Geng Yongzhi, Su Liaoyuan, Liu Keliang. COMPARISON OF TRANSFORMATION, DNA SINGLE STRAND BREAKS AND ITS REPAIR IN HUMAN BLOOD LYMPHOCYTES STIMULATED WITH PHA, ConA AND PWM FOLLOWING EXPOSURE TO γ-RAYS. [J]. Journal of Radiation Research and Radiation Processing 7(4):30-35(1989) DOI：

摘要

本文采用,3,H-TdR参入法和羟基磷灰石层析法，比较了,60,Coγ射线照射后PHA、ConA、PWM激活的人血淋巴细胞的转化、DNA单链断裂及其修复，结果表明：受照后淋巴细胞转化受抑，在0—8Gy剂量范围内，剂量效应呈双相线性关系，其中PWM激活的细胞对射线的敏感性最低。三种细胞受照后DNA发生单链断裂，在0—30 Gy范围内，与剂量呈线性相关，三者之间无显著差异。受15 Gy照射后，细胞在37℃条件下能重接DNA断链，但重接不完全，重接后如经较长时间保温仍会发生再断裂，PWM激活的细胞重接修复率最高。淋巴细胞转化对辐射的敏感性可能与DNA断链的重接修复能力有关。

Abstract

The transformation, DNA single strand breaks and its repair in human peripheral blood lymphocytes stimulated with PHA, ConA and PWM respectively following exposure to ,60,Co γ-rays were analyzed by ,3,H-thymidine incorporation and hydroxylapatite chromatography. The transformation of lymphocytes stimulated with PHA, ConA and PWM were suppressed by γ-rays and the dose-effect curves were biphasic within the range of 0-8 Gy. The lymphocytes stimulated with PWM were the most resistant to γ-rays. The extent of DNA single strand breaks in lymphocytes induced by γ-rays was linearly related to the dose within the range of 0-30 Gy and there was no marked difference in the three kinds of lymphocytes. After a period of post-irradiation (15 Gy) incubation at 37℃ the DNA single strand breaks could be rejoined but incompletely. The rejoined strands broke again if the cells were incubated longer. The repair ratio of single strand breaks in the lymphocytes stimulated with PWM was the highest among the cells with three mitogens. The results suggest that the difference of the radiation effect on transformation in different lytphocytes is probably related to the repair ability of DNA single strand breaks.

关键词

淋巴细胞DNA单链断裂DNA重接修复辐射敏感性PHAConAPWM

Keywords

LymphocyteDNA single strand breakDNA repairRadiosensitivityPHAConAPWM

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