INCREASE OF UV-RESISTANCE IN XERODERMA PIGMENTOSUM CELLS BY HUMAN HELAS3 DNA TRANSFECTION

Zhang Yangpei; Bai Xiaobin; Chen Yueneng; Xu Huiying; Fan Guocai; Xia Shouxuan

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INCREASE OF UV-RESISTANCE IN XERODERMA PIGMENTOSUM CELLS BY HUMAN HELAS3 DNA TRANSFECTION

更新时间：2023-04-23

- INCREASE OF UV-RESISTANCE IN XERODERMA PIGMENTOSUM CELLS BY HUMAN HELAS3 DNA TRANSFECTION
- Journal of Radiation Research and Radiation Processing Vol. 7, Issue 4, Pages: 25-29(1989)
- 作者机构：
  
  1.军事医学科学院放射医学研究所，北京
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Zhang Yangpei, Bai Xiaobin, Chen Yueneng, et al. INCREASE OF UV-RESISTANCE IN XERODERMA PIGMENTOSUM CELLS BY HUMAN HELAS3 DNA TRANSFECTION. [J]. Journal of Radiation Research and Radiation Processing 7(4):25-29(1989)
DOI：

Zhang Yangpei, Bai Xiaobin, Chen Yueneng, et al. INCREASE OF UV-RESISTANCE IN XERODERMA PIGMENTOSUM CELLS BY HUMAN HELAS3 DNA TRANSFECTION. [J]. Journal of Radiation Research and Radiation Processing 7(4):25-29(1989) DOI：

摘要

用电脉冲介导和DNA-磷酸钙共沉淀两种基因转移方法，将人HeLaS 3细胞DNA及限制性核酸内切酶切割后的DNA片段，与选择标记基因一同导入XP细胞，经两次选择后得到转化的细胞。实验证明HeLaS 3 DNA及经Bgl Ⅰ、Xho Ⅰ切割后的DNA片段可以修复XP细胞切除修复基因的缺陷，提高它们对紫外辐射的抗性。二次转化实验进一步证明，导入的HeLaS 3 DNA已在XP细胞基因组中整合，并得到稳定的表达。

Abstract

The DNA-mediated gene transfer had been carried out by both Calcium phosphate coprecipitation and electroporation method. The cellular DNA and DNA fragments from human cervical carcinoma HeLaS3 cells were introduced with PSV2Neo DNA into XP20S (SV40) cells. The transfectants were picked up after twice selections by G418 and 3J/m,2, UV-irradiation. The results showed that cellular DNA and Bgl Ⅰ, Xho Ⅰ digested DNA fragments from HeLaS 3 could correct the deficiency of excision repair gene in XP cells, and cause the recipient cells resistant to UV irradiation. The second transfection experiment confirmed that HeLaS3 DNA were really integrated into XP cell chromosome and stably expressed within the cell genome.

关键词

紫外辐射切除修复基因转移XP细胞HeLaS 3 DNA

Keywords

UV-irradiationExcision repairGene transferXP cellHeLaS3 DNA

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