EFFECT OF ADP-RIBOSYLATION ON TRANSCRIPTIONAL ACTIVITY IN RAT SPLEET NUCLEI AND RESPONSE TO IRRADIATION

Xu Hui; Yi Lukang

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EFFECT OF ADP-RIBOSYLATION ON TRANSCRIPTIONAL ACTIVITY IN RAT SPLEET NUCLEI AND RESPONSE TO IRRADIATION

更新时间：2023-04-23

- EFFECT OF ADP-RIBOSYLATION ON TRANSCRIPTIONAL ACTIVITY IN RAT SPLEET NUCLEI AND RESPONSE TO IRRADIATION
- Journal of Radiation Research and Radiation Processing Vol. 9, Issue 4, Pages: 210-213(1991)
- 作者机构：
  
  1.第三军医大学，重庆
- 作者简介：
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Xu Hui, Yi Lukang. EFFECT OF ADP-RIBOSYLATION ON TRANSCRIPTIONAL ACTIVITY IN RAT SPLEET NUCLEI AND RESPONSE TO IRRADIATION. [J]. Journal of Radiation Research and Radiation Processing 9(4):210-213(1991)
DOI：

Xu Hui, Yi Lukang. EFFECT OF ADP-RIBOSYLATION ON TRANSCRIPTIONAL ACTIVITY IN RAT SPLEET NUCLEI AND RESPONSE TO IRRADIATION. [J]. Journal of Radiation Research and Radiation Processing 9(4):210-213(1991) DOI：

摘要

本实验采用8 Gy γ线全身照射成年雄性Wistar大鼠，观察ADP-核糖化对大鼠脾细胞核转录活性的影响。结果表明核糖化对RNA pol（Ⅰ+Ⅲ）、polⅡ的活性均有抑制作用，并且对pol（Ⅰ+Ⅲ）的抑制率高于polⅡ，提示ADP-核糖化是脾细胞核RNA聚合酶，特别是polⅠ活性的影响因素；当核糖化液中加入ADPRT的特异性抑制剂3-AB，核糖化对转录活性的抑制作用消失，这进一步证实了RNA聚合酶活性的减少是由于核糖化所致。结果还表明照后4 h内，ADPRT活性降低，然后逐渐恢复，到18 h已完全恢复，表明8 Gy γ线照射可抑制ADPRT的活性，并且其辐射效应修复快。

Abstract

The effect of ADP-ribosylation on transcriptional activity in isolated nuclei from spleen of adult male rats irradiated with 8 Gy gamma-rays was studied. The results showed the activities of RNA pol(Ⅰ+Ⅲ) were all inhibited by ADP-ribosylation and this inhibition was lost in the presence of 3-AB, a specific inhibitor of ADPRT. It confirmed the effect of ADP-ribosylation. The fact that ADPRT activity decreased within 4 hours after irradiation and subsequently restored entirely after 18 hours showed irradiation of 8 Gy gamma ray inhibited the ADPRT activity and the effect of irradiation disappeared quickly.

关键词

ADP-核糖化转录辐射RNA聚合酶

Keywords

ADP-ribosylationTranscriptionIrradiationRNA polymerases

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