THE STUDY OF PREPARATION FOR IMMOBILIZED CELLS MEMBRANES OF E. COLI BY RADIATION TECHNIQUE

Cao Jin; Chen Pin; Yu Yi

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THE STUDY OF PREPARATION FOR IMMOBILIZED CELLS MEMBRANES OF E. COLI BY RADIATION TECHNIQUE

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- THE STUDY OF PREPARATION FOR IMMOBILIZED CELLS MEMBRANES OF E. COLI BY RADIATION TECHNIQUE
- Journal of Radiation Research and Radiation Processing Vol. 9, Issue 4, Pages: 219-222(1991)
- 作者机构：
  
  1.华东化工学院，上海
- 作者简介：
- 基金信息：
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Cao Jin, Chen Pin, Yu Yi. THE STUDY OF PREPARATION FOR IMMOBILIZED CELLS MEMBRANES OF E. COLI BY RADIATION TECHNIQUE. [J]. Journal of Radiation Research and Radiation Processing 9(4):219-222(1991)
DOI：

Cao Jin, Chen Pin, Yu Yi. THE STUDY OF PREPARATION FOR IMMOBILIZED CELLS MEMBRANES OF E. COLI BY RADIATION TECHNIQUE. [J]. Journal of Radiation Research and Radiation Processing 9(4):219-222(1991) DOI：

摘要

本文以大肠杆菌作为辐射制备固定化菌体膜的模型，采用尼龙为骨架，接枝甲基丙烯酸β羟乙酯（HEMA），使接枝后的尼龙具有亲水性，而后以低温包埋法固定大肠杆菌成为菌体膜。实验结果表明，当辐射剂量在10—12 kGy时，大肠杆菌膜保留活力最高；用50%HEMA单体浓度包埋时，其包埋量达2.3g/ml HEMA；菌体膜最适温度与最适pH与原始菌体基本相同。

Abstract

The paper decribed the preparation of immobilized cells membranes with E. Coli by radiation technique. The nylon 6 was grafted with HEMA, which as matrix to prepare immobilized cells membranes with E. Coli. by radiation entrapment at low temperature. The results showed that the retentive activity possessed a maxium value for membranes with E. Coli. when the irradiation dose was at 10—12 kGy, the entrapped cells has 2.3 g/ml at 50% HEMA concentration, the optimum pH and optimum temperature for membranes with E. Coli. are as same the original cells.

关键词

大肠杆菌辐射固定化甲基丙烯酸β-羟乙酯（HEMA）辐射接枝辐射包埋

Keywords

E. Coli.Radiation immobilizationHEMARadiation graftingRadiation entrapment

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