STUDT ON RADIATION-INDUCED DEACTIVATION AND POST-DEACTIVATION OF SOME OXIDO-REDUCTASES IN DILUTE AQUEOUS SOLUTIONS AND PROTECTIVE EFFECTS Ⅱ. RADIATION-INDUCED POST-DEACTIVATION OF CATALASE IN DILUTE AQUEOUS SOLUTIONS AND PROTECTIVE EFFECTS

Ha Hongfei; Chen Yiqing

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STUDT ON RADIATION-INDUCED DEACTIVATION AND POST-DEACTIVATION OF SOME OXIDO-REDUCTASES IN DILUTE AQUEOUS SOLUTIONS AND PROTECTIVE EFFECTS Ⅱ. RADIATION-INDUCED POST-DEACTIVATION OF CATALASE IN DILUTE AQUEOUS SOLUTIONS AND PROTECTIVE EFFECTS

更新时间：2023-04-23

- STUDT ON RADIATION-INDUCED DEACTIVATION AND POST-DEACTIVATION OF SOME OXIDO-REDUCTASES IN DILUTE AQUEOUS SOLUTIONS AND PROTECTIVE EFFECTS Ⅱ. RADIATION-INDUCED POST-DEACTIVATION OF CATALASE IN DILUTE AQUEOUS SOLUTIONS AND PROTECTIVE EFFECTS
- Journal of Radiation Research and Radiation Processing Vol. 11, Issue 2, Pages: 70-74(1993)
- 作者机构：
  
  1.北京大学技术物理系，北京　100871
- 作者简介：
- 基金信息：
  
  IAEA
- DOI：
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Ha Hongfei, Chen Yiqing. STUDT ON RADIATION-INDUCED DEACTIVATION AND POST-DEACTIVATION OF SOME OXIDO-REDUCTASES IN DILUTE AQUEOUS SOLUTIONS AND PROTECTIVE EFFECTS Ⅱ. RADIATION-INDUCED POST-DEACTIVATION OF CATALASE IN DILUTE AQUEOUS SOLUTIONS AND PROTECTIVE EFFECTS. [J]. Journal of Radiation Research and Radiation Processing 11(2):70-74(1993)
DOI：

Ha Hongfei, Chen Yiqing. STUDT ON RADIATION-INDUCED DEACTIVATION AND POST-DEACTIVATION OF SOME OXIDO-REDUCTASES IN DILUTE AQUEOUS SOLUTIONS AND PROTECTIVE EFFECTS Ⅱ. RADIATION-INDUCED POST-DEACTIVATION OF CATALASE IN DILUTE AQUEOUS SOLUTIONS AND PROTECTIVE EFFECTS. [J]. Journal of Radiation Research and Radiation Processing 11(2):70-74(1993) DOI：

摘要

报道了稀水溶液中过氧化氢酶在辐射后的放置期间，其生物活性仍随放置时间不断下降的后失活作用。研究了剂量、初始酶浓度、辐照气氛、温度对过氧化氢酶辐射后失活的影响以及添加剂对酶辐射后失活的抑制作用，发现乙醇、甲酸钠和络合剂EDTA均可有效地抑制酶的辐射后失活。样品在辐照和辐照后放置过程中氧的存在与否是辐射后失活现象的关键，而水辐解的分子产物H,2,O,2,对诱发这一后失活现象起有重要作用。

Abstract

In this work, the post-deactivation of irradiated catalase in dilute aqueous solution was found and investigated. Post-deactivation of irradiated catalase means that the catalase in dilute aqueous solution could not only be deactivated during γ-irradiation, but it has also been deactivated continously for some time after the irradiated samples were taken out of the radiation field. No reports about this phenmenon in literatures were searched up to now. The effects of absorbed dose,initial catalase concentration in solutions, atmosphere, temperature and additive on post-deactivation of catalase were investigated. H,2,O,2, produced by water radiolysis may attend the post-deactivation reaction in some way. Oxygen in ensyme samples is necessitous for the post-deactivation. 1×10,-4, to 5×10,-3, mol/L of CH,3,CH,2,OH, HCOONa and EDTA could control the post-deactivation efficiently.

关键词

过氧化氢酶乙醇、甲酸钠乙二胺四乙酸（EDTA）辐射后失活保护效应

Keywords

CatalaseAlcoholHCOONaEDTARadiation induced post-deactivationProtectire effect

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STUDY ON RADIATION-INDUCED DEACTIVATION AND POST-DEACTIVATION OF SOME OXIDOREDUCTASES IN DILUTE AQUEOUS SOLUTION AND PROTECTIVE EFFECT Ⅰ. RADIATIONINDUCED DEACTIVATION OF CATALASE IN DILUTE AQUEOUS SOLUTION AND PROTECTIVE EFFECT

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Shanghai Institute of Applied Physics, Chinese Academy of Sciences

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