PRIMARY STUDY ON MOLECULAR MECHANISM OF γ-INDUCED hprt GENE MUTATION IN MAMMALIAN CELLS

Ji Shouping; Fan Guocai; Wu Ying; Zhang Yangpei

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PRIMARY STUDY ON MOLECULAR MECHANISM OF γ-INDUCED hprt GENE MUTATION IN MAMMALIAN CELLS

更新时间：2023-04-23

- PRIMARY STUDY ON MOLECULAR MECHANISM OF γ-INDUCED hprt GENE MUTATION IN MAMMALIAN CELLS
- Journal of Radiation Research and Radiation Processing Vol. 12, Issue 3, Pages: 164-168(1994)
- 作者机构：
  
  1.军事医学科学院基础医学研究所，北京　100850
- 作者简介：
- 基金信息：
  
  National Natural Science Foundation of China
- DOI：
  CLC：
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Ji Shouping, Fan Guocai, Wu Ying, et al. PRIMARY STUDY ON MOLECULAR MECHANISM OF γ-INDUCED hprt GENE MUTATION IN MAMMALIAN CELLS. [J]. Journal of Radiation Research and Radiation Processing 12(3):164-168(1994)
DOI：

Ji Shouping, Fan Guocai, Wu Ying, et al. PRIMARY STUDY ON MOLECULAR MECHANISM OF γ-INDUCED hprt GENE MUTATION IN MAMMALIAN CELLS. [J]. Journal of Radiation Research and Radiation Processing 12(3):164-168(1994) DOI：

摘要

为研究电离辐射致突的分子机理，采用限制性片段长度多态性分析法（RFLPs）观察γ射线诱导的CHO-K1和HeLa MR突变细胞hprt基因结构的变化，并用Northern杂交和狭缝杂交方法分析了CHO-K1突变细胞hprt基因的表达情况。实验结果提示：电离辐射引起hprt基因结构损伤并进而导致mRNA转录的缺陷。

Abstract

By means of RFLPs method, the alterations of hprt gene structure in the mutant CHO-K 1 and HeLa MR cells induced by γ- exposure were examined in order to study the molecular mechanism of gene mutation induced by ionizing radiation: expression of hprt gene in CHO-K 1 mutant cells were also observed by Northern blot as well as slot blot. This experiment showed that ionizing radiation could result in hprt gene structure change which might lead to deficiency in mRNA transcription.

关键词

电离辐射hprt基因哺乳动物细胞RFLPs

Keywords

Ionizing radiationhprt geneMammalian cellRFLPs

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