RADIATION-INDUCED APOPTOSIS IN THYMOCYTES AS DETERMINED BY FLOW CYTOMETRY

Su Xu; Zhang Yingchun; Liu Shuzheng

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RADIATION-INDUCED APOPTOSIS IN THYMOCYTES AS DETERMINED BY FLOW CYTOMETRY

更新时间：2023-05-04

- RADIATION-INDUCED APOPTOSIS IN THYMOCYTES AS DETERMINED BY FLOW CYTOMETRY
- Journal of Radiation Research and Radiation Processing Vol. 13, Issue 2, Pages: 126-128(1995)
- 作者机构：
  
  1.白求恩医科大学卫生部放射生物实验室，长春　130021
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Su Xu, Zhang Yingchun, Liu Shuzheng. RADIATION-INDUCED APOPTOSIS IN THYMOCYTES AS DETERMINED BY FLOW CYTOMETRY. [J]. Journal of Radiation Research and Radiation Processing 13(2):126-128(1995)
DOI：

Su Xu, Zhang Yingchun, Liu Shuzheng. RADIATION-INDUCED APOPTOSIS IN THYMOCYTES AS DETERMINED BY FLOW CYTOMETRY. [J]. Journal of Radiation Research and Radiation Processing 13(2):126-128(1995) DOI：

摘要

自Wyllie等,[1],对程序性细胞死亡（PCD），又名细胞凋亡（apoptosis）的意义进行综述以来，对其发生机制的研究越来越多。普遍认为这种细胞死亡在胚胎发育、细胞增殖与分化以及肿瘤生长等研究方面均有重要意义。Cohen等,[2],对PCD在免疫系统中的作用进行了详细的评述。认为PCD的形态特点是核质浓缩，核膜完整而胞体变小，其分子标志是DNA裂解（fragmentation）,形成大小约180 bp或其倍数的断片，其行为特征是因细胞表面分子改变而被邻近的巨噬细胞吞噬，就地清除。因而它与另一种细胞死亡方式（坏死），具有截然不同的性质。

Abstract

Programmed cell death(PCD), or apoptosis, is a conceptually different way of cell death from necrosis. PCD plays an important role in immunologic regulation. PCD in thymocytes was analyzed by flow cytometry following in vitro X-irradiation. It was found that culturing of thymocytes could induce PCD which showed a time dependent increase. Four hours after culturing, 16% of thymocytes was found in the Ao region (PCD is shown in the Ao region of the histogram of flow cytometry). PCD in thymocytes showed a time dependent increase after 2.0 Gy X-irradiation, being significantly higher than that in the control at the same culturing time. 24 hours after X-irradiation in vitro, it was found that with 'doses below 100 mGy PCD was not significantly differrent from the control at the same culturing time. but when the doses were above 100 mGy, PCD showed a dose dependent increase, being significantly higher than that of the control at the same culturing time. These results are important in the understanding of the biological effects of low dose radiation.

关键词

程序性细胞死亡流式细胞术胸腺细胞X射线

Keywords

Programmed cell death (PCD)Flow cytometry (FCM)ThymocytesX-rays

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