STUDIES ON THE FUNCTION OF POKEWEED MITOGEN INDUCING LYMPHOCYTES AND <sup>60</sup>Co γ-RAYS IRRADIATION EFFECT

Xu Yindong; Su Liaoyuan; Liu Keliang

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STUDIES ON THE FUNCTION OF POKEWEED MITOGEN INDUCING LYMPHOCYTES AND ⁶⁰Co γ-RAYS IRRADIATION EFFECT

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- STUDIES ON THE FUNCTION OF POKEWEED MITOGEN INDUCING LYMPHOCYTES AND ⁶⁰Co γ-RAYS IRRADIATION EFFECT
- Journal of Radiation Research and Radiation Processing Vol. 8, Issue 1, Pages: 16-21(1990)
- 作者机构：
  
  苏州医学院，苏州
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：1990-02，
  
  Received：24 August 1988，
- 稿件说明：
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Xu Yindong, Su Liaoyuan, Liu Keliang. STUDIES ON THE FUNCTION OF POKEWEED MITOGEN INDUCING LYMPHOCYTES AND ⁶⁰Co γ-RAYS IRRADIATION EFFECT. [J]. Journal of Radiation Research and Radiation Processing, 1990,8(1):16-21.
DOI：

Xu Yindong, Su Liaoyuan, Liu Keliang. STUDIES ON THE FUNCTION OF POKEWEED MITOGEN INDUCING LYMPHOCYTES AND ⁶⁰Co γ-RAYS IRRADIATION EFFECT. [J]. Journal of Radiation Research and Radiation Processing, 1990,8(1):16-21. DOI：

摘要

应用玫瑰花环法分离人外周血T、B淋巴细胞，T

McAb标记淋巴细胞后经细胞亲和层析分离得到T

和非T

细胞。部分T、B、T

和非T

细胞接受10 Gy

线照射。将各种细胞匹配分组培养，以

H-TdR参入法观察PWM对淋巴细胞及其亚群激活功能和

Coγ线的辐射效应。结果表明T、B淋巴细胞均可被PWM激活，B强于T细胞；PWM对B和非T

细胞激活作用相当，唯T

细胞最弱。PWM激活时，T与B、T

与B细胞其DNA合成均有协同作用，前者的协同作用更大。当T、T

或B细胞受到10Gy

Co γ线照射后，它们之间的协同作用消失。非T

与B细胞间既无协同作用亦无抑制作用。10 Gy

Co γ线照射后，B和非T

细胞

H-TdR参入下降比T

细胞严重，前二者的差别不显著。

Abstract

T and B lymphocytes in human periphery blood were seperated by rosetting procedure. After lymphocytes were labelled with T

McAB

and non-T

lymphocytes were isolated by cell affinity chromatography method. When parts of T

B. T

and non-T

cells were exposed to 10 Gy

Co γ-rays

the function of pokeweed mitogen (PWM) and

Coγ-rays irradiation effect on lymphocytes were investigated by technique of

H-TdR incorporation.

The results showed that PWM can induce both T and B lymphocytes but B lymphocytes were more sensitive to PWM. The response of B lymphocytes to PWM was the same as that of non-T

cells

the response of T

cells to PWM was the lowest. DNA synthesis of both T and T

lymphocytes were synergistic to B lymphocytes. The synergistic effect in T and B lymphocytes was more strong than that in T

and B lymphocytes. When T and T

or B lymphocytes were irradiated by 10 Gy

Co γ-rays

the synergistic effect disappeared. Neither synergistic nor suppressive effect was found between non-T

and B lymphocytes. When T

B and non-T

lymphocytes had been exposed to 10 Gy irradiation

the percentage suppression of

H-TdR incorpora tion between B and non-T

lymphocytes had no significant difference but was higher than that in T

lymphocytes.

关键词

美州商陆丝裂原(PWM）单克隆抗体（McAb）淋巴细胞亚群辐射敏感性

Keywords

Pokeweed mitogen (PWM)Monoclonal antibody (McAb)Lymphocyte subpopulationRadiosensitivity

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University of Chinese Academy of Sciences

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⁰

STUDIES ON THE FUNCTION OF POKEWEED MITOGEN INDUCING LYMPHOCYTES AND 60Co γ-RAYS IRRADIATION EFFECT

DOI：

摘要

Abstract

关键词

Keywords

references

STUDIES ON THE FUNCTION OF POKEWEED MITOGEN INDUCING LYMPHOCYTES AND ⁶⁰Co γ-RAYS IRRADIATION EFFECT