EFFECT OF GAMMA IRRADIATION ON ERYTHROCYTE MEMBRANE IN RATS BY FLUORESCENT PROBES IN VITRO AND IN VIVO

Kang Xin; Fang Yunzhong

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EFFECT OF GAMMA IRRADIATION ON ERYTHROCYTE MEMBRANE IN RATS BY FLUORESCENT PROBES IN VITRO AND IN VIVO

更新时间：2024-11-05

- EFFECT OF GAMMA IRRADIATION ON ERYTHROCYTE MEMBRANE IN RATS BY FLUORESCENT PROBES IN VITRO AND IN VIVO
- Journal of Radiation Research and Radiation Processing Vol. 8, Issue 4, Pages: 199-202(1990)
- 作者机构：
  
  军事医学科学院放射医学研究所，北京
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：1990-11，
  
  Received：20 March 1989，
- 稿件说明：
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Kang Xin, Fang Yunzhong. EFFECT OF GAMMA IRRADIATION ON ERYTHROCYTE MEMBRANE IN RATS BY FLUORESCENT PROBES IN VITRO AND IN VIVO. [J]. Journal of Radiation Research and Radiation Processing, 1990,8(4):199-202.
DOI：

Kang Xin, Fang Yunzhong. EFFECT OF GAMMA IRRADIATION ON ERYTHROCYTE MEMBRANE IN RATS BY FLUORESCENT PROBES IN VITRO AND IN VIVO. [J]. Journal of Radiation Research and Radiation Processing, 1990,8(4):199-202. DOI：

摘要

ANS（1-苯胺-8-萘磺酸）和DPH（1，6-二苯基-1

3，5，-己三烯）作为探针，用于检测γ射线对大鼠红细胞膜的作用。离体照射大鼠红细胞膜，随着剂量的增加（10—40 Gy），ANS结合红细胞膜的荧光强度降低。用DPH作为荧光探针，在整体实验中也得到了同样的结果，全身照射8.5 Gy之后的第1、3和5 d，DPH结合大鼠红细胞膜的荧光强度下降，荧光偏振度增加。这些结果反映了照射使膜的流动性降低，可能是由于γ射线离体与整体照射诱导的膜不饱和脂肪酸的脂质过氧化及膜蛋白构象改变引起的。

Abstract

Fluorescent dyes

1-anilino-naphthalene sulfonate (ANS) and 1

6-diphenyl-1

5-hexatriene (DPH)

were used as probes to examine the effect of γ-irradiation on the rat erythrocyte membrane.

After irradiation of rat erythrocyte membrane in vitro

the fluorescence intensity of membrane-associated ANS decreased with the increase of irradiation dose (10-40 Gy).

Similar results were also observed in the experiment of irradiation in vivo as showed by the decrease of fluorescence intensity of DPH probe in the erythrocyte membrane as well as the increase of its polarization on the 1 st

3 rd

5 th day following whole body irradiation with 8.5 Gy.

Those results reflected the decrease of membrane fluidity which was suggested to be resulted from lipid peroxidation of polyunsaturated fatty acid in membrane induced by γ-irradiation in vitro and in vivo.

关键词

荧光探针红细胞膜流动性γ-射线离体照射整体照射

Keywords

Fluorescent probeErythrocyte membraneγ-rayIrradiation in vitroIrradiation in vivo

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