DETECTION OF RADIATION-INDUCED DNA DAMAGE AND INHIBITION OF REPAIR BY 3-AMINOBENZAMIDE USING FLUOROMETRIC ASSAY OF DNA UNWINDING

He Tao; Xia Shouxuan

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DETECTION OF RADIATION-INDUCED DNA DAMAGE AND INHIBITION OF REPAIR BY 3-AMINOBENZAMIDE USING FLUOROMETRIC ASSAY OF DNA UNWINDING

更新时间：2024-11-05

- DETECTION OF RADIATION-INDUCED DNA DAMAGE AND INHIBITION OF REPAIR BY 3-AMINOBENZAMIDE USING FLUOROMETRIC ASSAY OF DNA UNWINDING
- Journal of Radiation Research and Radiation Processing Vol. 8, Issue 4, Pages: 193-198(1990)
- 作者机构：
  
  放射医学研究所，北京
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：1990-11，
  
  Received：06 March 1989，
- 稿件说明：
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He Tao, Xia Shouxuan. DETECTION OF RADIATION-INDUCED DNA DAMAGE AND INHIBITION OF REPAIR BY 3-AMINOBENZAMIDE USING FLUOROMETRIC ASSAY OF DNA UNWINDING. [J]. Journal of Radiation Research and Radiation Processing, 1990,8(4):193-198.
DOI：

He Tao, Xia Shouxuan. DETECTION OF RADIATION-INDUCED DNA DAMAGE AND INHIBITION OF REPAIR BY 3-AMINOBENZAMIDE USING FLUOROMETRIC ASSAY OF DNA UNWINDING. [J]. Journal of Radiation Research and Radiation Processing, 1990,8(4):193-198. DOI：

摘要

采用简化的DNA解旋荧光法（FADU），检测HeLa S3细胞在受到γ射线照射后的DNA链断裂，在10 Gy以内，剂量效应曲线呈线性。ADP-核糖基转移酶特异性抑制剂3-氨基苯甲酰胺（3 AB），在无毒浓度下可以抑制HeLa S 3细胞DNA链断裂重接，并可降低HeLa S 3细胞照后活存率。3 AB单独与HeLa S 3细胞保温，不造成DNA链断裂，同时未见对细胞有毒性反应。3 AB可以作为有希望的放疗增敏剂深入研究。

Abstract

Simplified fluorometric assay of DNA unwinding was used to detect DNA strand breaks in HeLa S 3 cells after γ-irradiation. Linear dose-response curve was obtained within 10 Gy. In the presence of 3-aminobenzamide(3 AB)

a specific inhibitor of poly (ADP-ribose) transferase

the rejoining of DNA strand breaks in HeLa S 3 cells after

-irradiation was inhibited. 3AB also lowered the clone formation ability of HeLa S 3 cells after irradiation. No DNA strand breakage and cytotoxicity were seen when HeLa S 3 cells were treated with 3AB alone.

关键词

DNA链断裂重接ADP-核糖基转移酶3-氨基苯甲酰胺（3 AB）放射增敏

Keywords

DNAstrand break rejoiningPoly (ADP-ribose) transferase3-Aminobenzamide (3AB)Radiosensitization

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