EFFECTS OF THE CELL-FREE FILTRATES OF THE CRYPT CELL SUSPENSION ON THE CRYPT REGENERATION IN THE IRRADIATED MICE

Zeng Guiying; Han Shichen; Liu Jian; Liu Aiping; Zhou Yuankai

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EFFECTS OF THE CELL-FREE FILTRATES OF THE CRYPT CELL SUSPENSION ON THE CRYPT REGENERATION IN THE IRRADIATED MICE

更新时间：2024-11-05

- EFFECTS OF THE CELL-FREE FILTRATES OF THE CRYPT CELL SUSPENSION ON THE CRYPT REGENERATION IN THE IRRADIATED MICE
- Journal of Radiation Research and Radiation Processing Vol. 8, Issue 4, Pages: 228-230(1990)
- 作者机构：
  
  第四军医大学，西安
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：1990-11，
  
  Received：23 September 1989，
- 稿件说明：
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Zeng Guiying, Han Shichen, Liu Jian, et al. EFFECTS OF THE CELL-FREE FILTRATES OF THE CRYPT CELL SUSPENSION ON THE CRYPT REGENERATION IN THE IRRADIATED MICE. [J]. Journal of Radiation Research and Radiation Processing, 1990,8(4):228-230.
DOI：

Zeng Guiying, Han Shichen, Liu Jian, et al. EFFECTS OF THE CELL-FREE FILTRATES OF THE CRYPT CELL SUSPENSION ON THE CRYPT REGENERATION IN THE IRRADIATED MICE. [J]. Journal of Radiation Research and Radiation Processing, 1990,8(4):228-230. DOI：

摘要

用刮取法制备小鼠肠腺细胞悬液，分别经冻融和超声处理，并抽滤，获得肠腺无细胞滤液。BALB/c小鼠经15 Gy γ线全身照射后，注入肠腺无细胞滤液或肠腺细胞悬液。72 h后照射对照空肠段除有极少数残存肠腺外，已无肠腺再生；而照射注入组注入肠段有较多的新肠腺形成，肠腺内细胞生长良好，并可见未分化细胞。在9.4 Gy γ线腹部照射实验中观察到，照射并注入无细胞滤液后14 d时，新肠腺仍持续存在。本实验结果提示，在肠腺无细胞滤液中，存在着某种肠腺修复因子，它能促进受照射小鼠肠腺的修复再生。

Abstract

Two cell-free filtrates were obtained by filtering the fresh crypt cell suspensions which were pretreated with freeze-thawing and ultrasonic disintegration res

pectively through the G

funnel. 0.4 ml of each filtrate or the fresh crypt cell suspension was administered separately into the lumen of a 3 cm jejunal segment in BALB/c mouse 1-3 hours following 15 Gy γ-ray total-body irradiation. Seventy-two hours later

no regenerating crypt could be found and the villi become remarkably shorter in the irradiated control

while in the jejunal segments receiving both filtrates or the fresh crypt cell suspensions

numerous newly-formed crypts were visible with the villi apparently normal

meanwhile

the columnar

goblet

argentaffin

Paneth cells as well as undifferentiated cells could

be identified in the crypt region under electron microscope. The new crypts were found to exist persistently in 14 days' experimental period after 9.4 Gy γ-ray abdominal irradiation and filtrates administration. The above findings suggest the presence of a crypt repair factor in cell-free filtrates prepared from the intestinal crypt cell suspension of normal mice.

关键词

肠腺照射无细胞滤液再生

Keywords

Intestinal cryptIrradiationRegenerationCell-free filtrate

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