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ZHAO Jianghu; ZHUANG Wenzhuo; LONG Linmei; LI Feng; LIANG Zhongqin

doi:10.11889/j.1000-3436.2012.rrj.30.120107

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更新时间：2021-12-01

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- Journal of Radiation Research and Radiation Processing Vol. 30, Issue 1, Pages: 37-41(2012)
- 作者机构：
  
  1. （苏州大学医学部药理学系）,苏州,215123
- 作者简介：
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- DOI：10.11889/j.1000-3436.2012.rrj.30.120107
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ZHAO Jianghu, ZHUANG Wenzhuo, LONG Linmei, et al. [J]. Journal of Radiation Research and Radiation Processing 30(1):37-41(2012)
DOI：

ZHAO Jianghu, ZHUANG Wenzhuo, LONG Linmei, et al. [J]. Journal of Radiation Research and Radiation Processing 30(1):37-41(2012) DOI： 10.11889/j.1000-3436.2012.rrj.30.120107.

摘要

为探讨自噬调节对胶质瘤起始细胞移植瘤辐射敏感性的影响，建立裸小鼠皮下移植瘤模型，测量肿瘤体积大小，比较抑瘤率检测雷帕霉素对移植瘤的辐射增敏作用；建立裸小鼠颅内移植瘤模型，观察裸小鼠生存期，HE染色观察移植瘤的生长。取两种模型中各组肿瘤组织做Western blot，分析自噬相关蛋白LC3表达。结果显示，裸小鼠皮下移植瘤模型中，联合治疗组的肿瘤体积明显小于阴性对照组和单纯辐照组（P>0.05），抑瘤率明显增加（P>0.05）；在裸小鼠颅内原位移植瘤模型中，联合治疗组的裸小鼠生存期较阴性对照组和单纯辐照组明显延长，HE染色中可见联合治疗组肿瘤生长局限，与周围正常脑组织界限清楚。Western blot结果显示，两种模型中，联合治疗组中的比值LC3II /LC3I显著增加。研究表明，自噬激动剂雷帕霉素通过激活胶质瘤起始细胞的自噬活性，增加胶质瘤的辐射敏感性。

Abstract

To investigate the effect of rapamycin (RPM) on radio-sensitivity of glioma - initiating cells (GICs) in vivo, GICs were inoculated subcutaneously into the NC nude mice. And an intracranial orthotropic model was utilized for evaluation of radio-sensitivity by use of orthotropic injection. Protein levels of LC3 were analyzed with immunoblotting. It was found that tumor inhibition ratio of RPM+IR group was higher than that in control group and IR group. Combination treatment of nude mice bearing intracranial GICs glioma xenografts with rapamycin and IR made a significant increment of animal survival compared with control or IR alone group. Western blot assay showed that the rate of LC3?II /LC3-I increased obviously in combination treatment group among two models. As a conclusion, Rapamycin can enhance the radio-sensitivity of glioma-initiating cells by inducing autophagy in vivo.Cited

关键词

胶质瘤起始细胞自噬辐射增敏雷帕霉素体内实验

Keywords

Glioma-initiating cellsAutophagyRadio-sensitivityRapamycinIn vivo test

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The Clinical College of Chongqing Medical University

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DOI：10.11889/j.1000-3436.2012.rrj.30.120107

摘要

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关键词

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