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SHEN Linling; ZHAO Lin; MA Liang; YANG Jiangtao; JIAO Yang; XU Jiaying; FAN Saijun

doi:10.11889/j.1000-3436.2012.rrj.30.120108

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- Journal of Radiation Research and Radiation Processing Vol. 30, Issue 1, Pages: 42-47(2012)
- 作者机构：
  
  1. （苏州大学医学部放射医学与防护学院苏州市肿瘤放射生物学重点实验室）,苏州,215123
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- DOI：10.11889/j.1000-3436.2012.rrj.30.120108
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SHEN Linling, ZHAO Lin, MA Liang, et al. [J]. Journal of Radiation Research and Radiation Processing 30(1):42-47(2012)
DOI：

SHEN Linling, ZHAO Lin, MA Liang, et al. [J]. Journal of Radiation Research and Radiation Processing 30(1):42-47(2012) DOI： 10.11889/j.1000-3436.2012.rrj.30.120108.

摘要

探讨四环素衍生物CMT-8对人宫颈癌细胞HeLa的生长以及放射增敏性的影响。分别采用MTT法、伤口愈合实验、细胞粘附实验、流式细胞术和Western blot法测定CMT-8对HeLa细胞生长、迁移、粘附、周期的影响及其相关蛋白表达水平的改变。实验结果表明，CMT-8可有效抑制HeLa细胞的生长，降低其迁移、粘附能力，引起细胞G0/G1期阻滞、下调周期蛋白Cyclin D和Cyclin E的表达水平。CMT-8联合X?射线能明显抑制细胞的生长，下调DNA损伤修复蛋白Ku70和DNA-PKcs的表达水平。这些发现提示，CMT-8作为人宫颈癌抗肿瘤新药可能是与其抑制细胞生长、细胞周期调控和抗迁移有关。另外，CMT-8在低剂量下可以提高人宫颈癌细胞的放射敏感性，与其调节DNA损伤修复蛋白的表达水平有关。

Abstract

To investigate the effects of chemically modi?ed tetracycline 8 (CMT-8) on cell growth, migration and radio-senstivity in human cervical cancer HeLa cells, MTT assay was used to determine cell viability and radiosensitivity; wound healing and adhesive assays were used to measure cell migration and adhesion; flow cytometry and Western blot assay were performed to analyze cell cycle alteration and protein expression. The results showed that CMT-8 inhibited HeLa cell growth, migration and adhesion in a dose-and time dependent fashion. Furthermore, CMT-8 caused a G0/G1 arrest, in accompany with a dose-dependent down-regulation of Cyclin D and Cyclin E protein expression. Finally, HeLa cells pre-treated with CMT-8 at non-cytotoxic concentrations became more sensitive to subsequent irradiation of X-rays, which was related to a decreased expression of Ku70 and DNA-PKcs protein. Taken together, these findings indicate that CMT-8 is a new anti-cervical cancer compound via inhibiting cell growth and migration as well as mediating radio-sensitivity.Cited

关键词

CMT-8HeLa细胞X射线放射敏感性

Keywords

CMT-8HeLa cellX-raysRadiosensitivity

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School of Radiation Medicine and Public Health, Medical College of Suzhou University, Jiangsu Provincial key Laboratory of Radiation Medicine and Protection

(The Key Laboratory of Radiation Medicine and Protection of Jiangsu Province, School of Radiation Medicine and Protection,Medical College of Soochow University,,)

Medical College of Northwest Minzu University, Laboratory of Environmental Ecology and Population Health in Northwest Minority Regions of Northwest Minzu University

Department of Radiation Oncology, The First Affiliate Hospital of Anhui University of Science and Technology

First Clinical Hospital, School of Medicine, Anhui University of Science and Technology

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DOI：10.11889/j.1000-3436.2012.rrj.30.120108

摘要

Abstract

关键词

Keywords

references