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HU Wei; CHEN Jihong; ZHANG Zhen; LIU Jing; WANG Shuyang; LI Wenjian

doi:10.11889/j.1000-3436.2012.rrj.30.120110

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更新时间：2021-12-01

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- Journal of Radiation Research and Radiation Processing Vol. 30, Issue 1, Pages: 53-57(2012)
- 作者机构：
  
  1. 1（甘肃农业大学食品科学与工程学院）,兰州,730030
  2. 2（中国科学院近代物理研究所）,兰州,730000
- 作者简介：
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- DOI：10.11889/j.1000-3436.2012.rrj.30.120110
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HU Wei, CHEN Jihong, ZHANG Zhen, et al. [J]. Journal of Radiation Research and Radiation Processing 30(1):53-57(2012)
DOI：

HU Wei, CHEN Jihong, ZHANG Zhen, et al. [J]. Journal of Radiation Research and Radiation Processing 30(1):53-57(2012) DOI： 10.11889/j.1000-3436.2012.rrj.30.120110.

摘要

应用不同剂量重离子束12C+6对黑曲霉出发菌株4﹟进行辐照选育，选育出的高产酸菌株进行发酵试验，应用酸碱中和法测定发酵液中柠檬酸的含量。结果表明：当重离子12C+6的辐照剂量为 60 Gy时，正突变率最大，为21%。诱变后的菌株通过酸斑法初筛、摇瓶复筛选育出1株菌株H4002，其发酵液中柠檬酸含量比出发菌株4﹟提高了18%。这说明利用重离子束12C+6辐照处理黑曲霉产生菌可以得到高产柠檬酸突变菌株，其诱变效果显著。

Abstract

In order to screen high-producing-citric acid strains, the original strain 4# was irradiated with different doses of heavy ion beams 12C+6. The citric acid content in the fermentation broth was measured by acid-base neu-tralization. The results showed that the positive mutation rate reached the highest value of 21% as the heavy ion 12C+6 irradiation dose was 60 Gy, and the high producing citric acid strain H4002 was obtained after screening. Compared with the original strain, its fermentation content of citric acid was improved by 18%. The results suggest that high-producing-citric acid strain could be obtained after it was irradiated by heavy ion beams and the mutation effect was remarkable.Cited

关键词

黑曲霉重离子束诱变选育

Keywords

Aspergillus nigerHeavy ion beamMutationBreeding

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Institute of Modern Physics, Chinese Academy of Sciences

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DOI：10.11889/j.1000-3436.2012.rrj.30.120110

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Abstract

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