暂无标题

HE Genlin; YANG Xuesen; ZHANG Guangbin; YU Zhengping

doi:10.11889/j.1000-3436.2012.rrj.30.120111

您当前的位置：

首页 >

文章列表页 >

暂无标题

更新时间：2021-12-01

- 暂无标题
- Journal of Radiation Research and Radiation Processing Vol. 30, Issue 1, Pages: 58-64(2012)
- 作者机构：
  
  1. 1（第三军医大学热带医学研究所）,重庆,400038
  2. 2（第三军医大学劳动卫生学教研室电磁辐射医学防护教育部重点实验室）,重庆,400038
- 作者简介：
- 基金信息：
- DOI：10.11889/j.1000-3436.2012.rrj.30.120111
  CLC：
扫描看全文
HE Genlin, YANG Xuesen, ZHANG Guangbin, et al. [J]. Journal of Radiation Research and Radiation Processing 30(1):58-64(2012)
DOI：

HE Genlin, YANG Xuesen, ZHANG Guangbin, et al. [J]. Journal of Radiation Research and Radiation Processing 30(1):58-64(2012) DOI： 10.11889/j.1000-3436.2012.rrj.30.120111.

摘要

为探讨微波辐照致小胶质细胞活化后吞噬功能变化及其与MFG-E8表达的关系，采用60 mW/cm2的脉冲微波辐照N9小胶质细胞，ELISA技术测定TNF-α含量，硝酸还原酶法测定NO含量，免疫荧光检测CD11b和MFG-E8，Western blot 检测MFG-E8蛋白表达。与正常对照组相比，辐照后3、12 h小胶质细胞中CD11b的表达明显增加（p<0.05），TNF-α和NO含量辐照后1 h即明显升高，并持续至辐照后24 h，小胶质细胞吞噬功能在辐照后3 h至6 h增强（p<0.05），12 h反而低于正常对照组（p<0.05），而MFG-E8蛋白表达与吞噬功能变化趋势相似。结果表明，60 mW/cm2的脉冲微波辐照可明显诱导小胶质细胞活化，活化后小胶质细胞对凋亡细胞的吞噬功能出现先增强后减弱的变化，而MFG-E8蛋白表达变化可能是其吞噬功能改变的主要原因之一。

Abstract

In order to investigate the effects of microwave on the dynamic changes of phagocytic function and Milk Fat Globule Factor-E8 (MFG-E8) expression in microglia, N9 microglial cells were irradiated with a 20-min exposure to 60 mW/cm2 pulsed microwave or a sham treatment. TNF-α and NO were measured using enzymelinked immunosorbent assay (ELISA) and the nitrate reductase method, respectively. Microglial marker CD11b and the bridging molecule, MFG-E8 was assessed via immunoreaction. Expression of MFG-E8 protein was evaluated by western blotting. CD11b positive N9 cells were significantly increased at 3 h and 12 h (p<0.05). The secretion of TNF-α and NO were significantly increased at 1 h after microwave exposure (p<0.05), then maintained at a high level for 24 h. Phagocytic function was enhanced at 3 h and 6 h after microwave exposure (p<0.05), then decreased, even lower than the sham group at 12 h (p<0.05). The expression pattern of MFG-E8 was similar with the phagocytic func-tion. These results indicate that microwave can obviously activate microglia, the ability of the phagocytiosis of apoptotic cells is increased first, and then decreased in the over-activated microglia. MFG-E8 expression pattern may be responsible for the radiation-induced change of microglial phagocytosis.Cited

关键词

微波小胶质细胞活化吞噬MFG-E8

Keywords

MicrowaveMicrogliaActivationPhagocytosisMFG-E8

references

查看原文

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Relationship between cognitive impairment induced by local radiation and morphological changes of hippocampal microglia

Biological effects of 2450 MHz microwave combined with γ-rays on rat cultured gliacytes

The detection of hot water extraction from paper raw material using microwave technology

The reduction of γ-rays damages of mice hematopoietic system pre-treated by low dose microwave irradiation

The influence of microwave irradiation during lactation and gestation on the role of thyroid hormone in dysfunction of learning and memory with mice

Related Author

No data

Related Institution

Clinical Anatomy & Reproductive Medicine Application Institute, School of Medicine, University of South China

Key Laboratory of Brain Science Research & Transformation in Tropical Environment of Hainan Province, Hainan Medical University

Cancer Research Institute, Key Laboratory of Cancer Cellular and Molecular Pathology of Hunan Provincial, Hengyang Medical College, University of South China

School of Radiation Medicine and Public Health, Suzhou University

Changzhou Center for Disease and Control Prevention

⁰

暂无标题

DOI：10.11889/j.1000-3436.2012.rrj.30.120111

摘要

Abstract

关键词

Keywords

references