暂无标题

GUO Jianping; ZHANG Huaping; AN Quan; WEI Jinping; ZHOU Jinyuan; YANG Zhongtian

doi:10.11889/j.1000-3436.2012.rrj.30.120209

您当前的位置：

首页 >

文章列表页 >

暂无标题

更新时间：2021-12-01

- 暂无标题
- Journal of Radiation Research and Radiation Processing Vol. 30, Issue 2, Pages: 108-112(2012)
- 作者机构：
  
  1. 1（中国辐射防护研究院）,太原,030006
  2. 2（山西医科大学分子教研室）,太原,030001
- 作者简介：
- 基金信息：
- DOI：10.11889/j.1000-3436.2012.rrj.30.120209
  CLC：
扫描看全文
GUO Jianping, ZHANG Huaping, AN Quan, et al. [J]. Journal of Radiation Research and Radiation Processing 30(2):108-112(2012)
DOI：

GUO Jianping, ZHANG Huaping, AN Quan, et al. [J]. Journal of Radiation Research and Radiation Processing 30(2):108-112(2012) DOI： 10.11889/j.1000-3436.2012.rrj.30.120209.

摘要

从细胞凋亡角度探讨低聚壳聚糖提高辐射损伤动物存活率的机理。小鼠受到5 Gy γ射线照射后，检测骨髓淋巴细胞凋亡数、脾脏细胞凋亡相关基因和P53蛋白的变化。流式细胞仪检测细胞凋亡，RT-PCR技术检测凋亡基因，Western方法检测蛋白表达。与单纯照射组相比，低聚壳聚糖提高受照小鼠的存活率，降低骨髓淋巴细胞的凋亡细胞数量；下调凋亡相关基因Bax、caspas-3，上调Bc1-2，Bcl-2/Bax比值升高；抑制P53蛋白表达。低聚壳聚糖可能通过抑制P53蛋白表达，影响凋亡相关基因表达，从而抑制凋亡细胞的产生，有效提高受照小鼠存活率。

Abstract

In order to study the mechanism of oligo-chitosan boosting survival rate with irradiated mice, the apoptosis number of borrow leukomo-nocyte cell, apoptosis related gene and P53 protein expression of the mice after 5 Gy γ-rays irradiation was investigated respectively. The cell apoptosis was analyzed by flow cytometry. Apoptosis related gene was determined by Real Time-Polymerase Chain Reaction (RT-PCR) and Western blot assay was used to examine the expression of P53. Compared with the control group, the results show that oligo-chitosan can either in-crease the survival rate or decrease the number of apoptosis and apoptosis related gene of Bax caspas-3, while Bc1-2 and Bcl-2/Bax ratio increase and the expression of P53 is restrained. It can be deduced that oligo-chitosan will influence the apoptosis related gene because of refraining the expression of P53 and leaded to shutdown the number of apoptosis, which then improve the survival rate of irradiated mice effectively.Cited

关键词

低聚壳聚糖辐射细胞凋亡存活率

Keywords

Oligo-chitosanRadiationApoptosisSurvival rate

references

查看原文

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

EFFECT OF RADIATION ON APOPTOSIS IN SMALL INTESTINE AND COLON OF MICE

Trends and levels of environmental radiation in Shenzhen from 2011 to 2020

Review of the synthesis of semiconductor nanomaterials using ionizing radiation technology

Bioinformatics analysis of differentially expressed genes in irradiated non-small cell lung cancer

Protective effect of compound raspberry seed powder on radiation-induced spinal cord injury in mice

Related Author

No data

Related Institution

Dept. of Radiation Medicine, the Fourth Military Medical University

Shenzhen Ecological and Environmental Monitoring Center of Guangdong Province

College of Chemistry and Chemical Engineering, Hunan Normal University

College of Resources and Environment, Hunan Agricultural University

Institute of Nuclear Agricultural Science and Space Mutation Breeding, Hunan Academy of Agriculture Sciences

⁰

暂无标题

DOI：10.11889/j.1000-3436.2012.rrj.30.120209

摘要

Abstract

关键词

Keywords

references