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LIU Yunying1; ZHOU Xiaowang1; WAN Jianmei2; HU Danfei1; ZHANG Youjiu1

doi:10.11889/j.1000-3436.2012.rrj.30.120510

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更新时间：2021-12-01

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- Journal of Radiation Research and Radiation Processing Vol. 30, Issue 5, Pages: 309-315(2012)
- 作者机构：
  
  1. 1（苏州大学医学部放射医学与防护学院江苏省放射医学与防护重点实验室）,苏州,215123
  2. 2（苏州大学医学部实验中心）,苏州,215123
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- DOI：10.11889/j.1000-3436.2012.rrj.30.120510
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LIU Yunying1, ZHOU Xiaowang1, WAN Jianmei2, et al. [J]. Journal of Radiation Research and Radiation Processing 30(5):309-315(2012)
DOI：

LIU Yunying1, ZHOU Xiaowang1, WAN Jianmei2, et al. [J]. Journal of Radiation Research and Radiation Processing 30(5):309-315(2012) DOI： 10.11889/j.1000-3436.2012.rrj.30.120510.

摘要

为研究WSC-DTPA（水溶性低分子量壳聚糖WSC，二乙烯三胺五乙酸DTPA）纳米粒的辐射防护作用，采用N-乙酰化反应和离子凝胶法制备不同游离氨基含量的WSC-DTPA纳米粒；MTT法检测其对6 Gy 60Co γ射线照射后48 h BRL细胞存活率的影响；活细胞工作站观察BRL细胞摄取FITC-WSC-DTPA纳米荧光探针的情况。结果表明：成功合成了游离氨基含量分别为92.7%、74.3%、1.59%的WSC-DTPA聚合物；WSC、WSC纳米粒以及WSC-DTPA纳米粒（氨基含量为92.7%，浓度在6.25 μg/mL以上），随着药物浓度的增加，BRL细胞存活率均显著高于单纯照射组，差别有统计学意义（p<0.05），而游离氨基含量为1.59% 的WSC-DTPA纳米粒无辐射保护作用；活细胞工作站检验结果显示2 h内WSC纳米粒、WSC-DTPA纳米粒能够进入BRL细胞，而非纳米化的WSC-DTPA聚合物无法进入细胞。

Abstract

To investigate the radiation protection of WSC-DTPA nanoparticles, N-acylation reaction and ion crossing-linking method were used to prepare nanoparticles in different remaining free amino groups. MTT assay was performed to evaluate their effects on the viability of the cells after γ-rays irradiation, while the capability of FITC-WSC-DTPA nanoparticles probe penetrating through cellular membrane was observed by Live Cell Station. The results show that WSC-DTPA polymers were successfully synthesized, in which the content free amino group is 92.7%, 74.3%, 1.59%, respectively. Compared with γ-rays alone group, it was shown that WSC, WSC nanoparticles and WSC-DTPA nanoparticles with the concentration more than 6.25 μg/mL and the free amino group content around 92.7% have good efficacy in radiation protection to BRL cells from damage induced by 6 Gy 60Co γ-rays by the higher survival rate, while WSC-DTPA nanoparticles with 1.59% free amino group content do not have radiation protection to BRL cells. The WSC nanoparticles and WSC-DTPA nanoparticles can penetrate into cellular within 2 h, while WSC-DTPA polymers could not pass through the membrane.Cited

关键词

水溶性低分子量壳聚糖（WSC）二乙烯三胺五乙酸（DTPA）纳米粒BRL细胞辐射保护

Keywords

Water-soluble low-molecular weight chitosan(WSC)Diethylenetriamine pentaacetatic acid (DTPA)NanoparticlesBRL cellsRadiation protection

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Radiation protective property of water-soluble chitosan with low-molecular weight

RADIATION PROTECTION TO IRRADIATED ANLMALS BY THE TRATMENT OF ESTRADIOL BENZOATE AND CHINESE MADICINE OF TONIFYING VITAL ENERGY AND ELIMINATING THE STAGMENT

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School Of Radiation Medicine and Public Health, Medical College, Soochow University, Jiangsu Province Key Laboratory of Radiation Medicine and Protection

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DOI：10.11889/j.1000-3436.2012.rrj.30.120510

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