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LIU Ruiyuan; DONG Xicun; LI Wenjian; HE Jinyu; QU Ying; JIN Wenjie

doi:10.11889/j.1000-3436.2012.rrj.30.120607

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- Journal of Radiation Research and Radiation Processing Vol. 30, Issue 6, Pages: 353-358(2012)
- 作者机构：
  
  1. （中国科学院近代物理研究所）,兰州,730000
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- DOI：10.11889/j.1000-3436.2012.rrj.30.120607
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LIU Ruiyuan, DONG Xicun, LI Wenjian, et al. [J]. Journal of Radiation Research and Radiation Processing 30(6):353-358(2012)
DOI：

LIU Ruiyuan, DONG Xicun, LI Wenjian, et al. [J]. Journal of Radiation Research and Radiation Processing 30(6):353-358(2012) DOI： 10.11889/j.1000-3436.2012.rrj.30.120607.

摘要

为研究重离子辐照突变株的细胞遗传学变化，以碳离子辐照选育出的甜高粱早熟突变株 KFJT-1及其对照品种 KFJT-CK为材料，比较研究了两者之间花粉生活力大小以及花粉母细胞减数分裂的差异。结果发现：KFJT-1的花粉生活力以及花粉总数大于KFJT-CK；KFJT-1花粉母细胞减数分裂大多进入第二次减数分裂时，KFJT-CK花粉母细胞集中在第一次减数分裂；在KFJT-1减数分裂末期Ⅱ，出现了两组分裂时期不一致、3分体以及不均等分裂畸变状况，畸变率仅为4.5%，说明KFJT-1在生长繁殖过程中修复了大部分由于物理诱变造成的染色体畸变，且能稳定遗传。这些结果从生殖细胞水平进一步说明碳离子辐照早熟突变株KFJT-1较未突变株KFJT-CK成熟期早，且各项指标均优于KFJT-CK。

Abstract

To investigate the change of mutanted cytogenetics, KFJT-1 irradiated by carbon ions and KFJT-CK were used and the difference of pollen viability and pollen mother cells meiosis were comparatively studied. The ex-perimental results showed that the total number of pollens and pollen viability of KFJT-1 were more than that of KFJT-CK. When the meiosis of pollen mother cells were mostly turned into the second meiotic division for KFJT-1, the meiosis of KFJT-CK was being at the stage of the first meiotic division. Triad, unequal separation of chromosome and asychronization of chromosomes segregation were found in KFJT-1 meiosis Ⅱ, and the aberration rate of KFJT-1 was only 4.5%, which indicated that the most of chromosomal aberrations induced by carbon ions were repaired and the genetic characteristics were stabilized during the growth of reproduction and might be stable genetic. These results show that KFJT-1 maturity is earlier than KFJT-CK, and all of indexes are better than the KFJT-CK.Cited

关键词

甜高粱碳离子花粉生活力减数分裂

Keywords

Sweet sorghumCarbon ion beamPollen viabilityMeiosis

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Institute of Modern Physics, Chinese Academy of Sciences

Graduate School of Chinese Academy of Sciences

1 (Gansu Agricultural University,,)

2 (Institute of Modern Physics, Chinese Academy of Sciences,,)

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DOI：10.11889/j.1000-3436.2012.rrj.30.120607

摘要

Abstract

关键词

Keywords

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