暂无标题

TANG Quan1; YANG Bing1; ZHONG Leilei1; YANG Fujun1; ZHANG Xiaodong1; SHANG Jie2; WANG Qin1; HAN Ying1; FAN Tiqiang1; FAN Feiyue1; SUN Yuanming1

doi:10.11889/j.1000-3436.2012.rrj.30.120611

您当前的位置：

首页 >

文章列表页 >

暂无标题

更新时间：2021-12-01

- 暂无标题
- Journal of Radiation Research and Radiation Processing Vol. 30, Issue 6, Pages: 374-377(2012)
- 作者机构：
  
  1. 1（北京协和医学院&中国医学科学院放射医学研究所天津分子核医学重点实验室）,天津,300192
  2. 2（天津医科大学总医院药剂科）,天津,300052
- 作者简介：
- 基金信息：
- DOI：10.11889/j.1000-3436.2012.rrj.30.120611
  CLC：
扫描看全文
TANG Quan1, YANG Bing1, ZHONG Leilei1, et al. [J]. Journal of Radiation Research and Radiation Processing 30(6):374-377(2012)
DOI：

TANG Quan1, YANG Bing1, ZHONG Leilei1, et al. [J]. Journal of Radiation Research and Radiation Processing 30(6):374-377(2012) DOI： 10.11889/j.1000-3436.2012.rrj.30.120611.

摘要

揭示了电离辐射对成骨细胞系MC3T3细胞的增殖及其对Notch1和Jagged1基因表达的影响。成骨细胞系MC3T3细胞，经过0、2和4 Gy 137Cs γ射线照射，运用MTT、实时定量PCR技术，检测电离辐射对与MC3T3细胞的增殖和增殖分化相关基因Notch1和Jagged1表达的影响。照射剂量为2 和4 Gy时，细胞的增殖下降（p<0.05，p<0.01）；照射剂量为2 Gy时，Notch1基因表达量降低（p<0.05）；照射剂量为2 和4 Gy时，Jagged1基因的表达量均降低（p<0.01，p<0.05）。电离辐射损伤使成骨细胞系MC3T3的增殖能力下降，同时诱发Notch1基因和Jagged1的低表达。

Abstract

In order to investigate the effects of ionizing radiation on MC3T3 cells’ proliferation and expression changes of Notch1 and Jagged1 genes, MC3T3 cells were exposed to γ-rays with 0, 2 and 4 Gy, respectively. MTT assay and real-time PCR were adopted to detect the proliferation of MC3T3 cells and the changes of expression of Notch1 and Jagged1 genes which could be in association with proliferation and differentiation. When exposed to 2 and 4 Gy γ-rays irradiation, the MC3T3 cells' proliferation reduced (p<0.01, p<0.01). The expression of Notch1 decreased when treated with 2 Gy irradiation (p<0.05). The expression of Jagged1 regulated down when given 2 and 4 Gy irradiation (p<0.01, p<0.05). Irradiated MC3T3 cells’ proliferation potentialities reduced and Notch1 and Jagged1were regulated down.Cited

关键词

电离辐射成骨细胞MC3T3Notch1Jagged1

Keywords

Ionizing radiationOsteoblastMC3T3Notch1Jagged1

references

查看原文

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

辐照骨髓淋巴细胞对成骨细胞的影响

局部辐照大鼠血清对成骨细胞增殖分化的影响

Preparation of hydrogel-based photocatalytic composite materials by radiation technology

Clustered DNA damages induced by low-energy (1~20 eV) electrons and the general mechanism of transient anions

Research progress on the effect of water types on H₂ production in cement solidification treatment

Related Author

No data

Related Institution

(Institute of Radiation Medicine of Fudan University,,)

School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology

Hubei Key Laboratory of Radiation Chemistry and Functional Materials, Hubei University of Science and Technology

College of Pharmacy, Hubei University of Science and Technology

⁰

暂无标题

DOI：10.11889/j.1000-3436.2012.rrj.30.120611

摘要

Abstract

关键词

Keywords

references