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YUAN Xiaopeng; ZENG Chuchu; XING Lihua; ZHAO Xiaojun; GU Cheng; YU Jiahua; LIU Fenju

doi:10.11889/j.1000-3436.2014.rrj.32.010201

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更新时间：2021-12-01

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- Journal of Radiation Research and Radiation Processing Vol. 32, Issue 1, Pages: 10201-010201(2014)
- 作者机构：
  
  1. （苏州大学医学部放射医学与防护学院）,苏州,215123
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- DOI：10.11889/j.1000-3436.2014.rrj.32.010201
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YUAN Xiaopeng, ZENG Chuchu, XING Lihua, et al. [J]. Journal of Radiation Research and Radiation Processing 32(1):10201-010201(2014)
DOI：

YUAN Xiaopeng, ZENG Chuchu, XING Lihua, et al. [J]. Journal of Radiation Research and Radiation Processing 32(1):10201-010201(2014) DOI： 10.11889/j.1000-3436.2014.rrj.32.010201.

摘要

采用CoCl2处理U251细胞模拟细胞乏氧，用甲基四唑蓝(MTT)法检测U251细胞活力的改变；RT-PCR和Western blot检测HIF-1α在U251细胞中的mRNA和蛋白水平的表达；克隆形成法检测X射线照射下不同处理组的克隆形成率。结果表明，100 µmol•L−1 CoCl2 处理及100 µmol•L−1 CoCl2与0.5 µmol•L−1 WP1066联合处理U251细胞24 h无明显细胞毒性作用(p>0.05)；CoCl2 诱导HIF-1α的mRNA及蛋白表达水平上调，WP1066抑制HIF-1α在蛋白水平的表达；与空白对照组相比，CoCl2组的克隆形成能力明显上调(p<0.05)，CoCl2与WP1066联合处理组克隆形成能力明显下降(p<0.05)。WP1066可增加乏氧U251细胞放射敏感性，其机制可能是通过抑制STAT3通路降低HIF-1α的表达而发挥放射增敏作用。

Abstract

To investigate the effects of WP1066, an STAT3 inhibitor, on the radiosensitivity of hypoxic U251 human glioma cells induced by cobalt chloride (CoCl2), the viability of U251 cells inhibited by CoCl2 and WP1066 was examined by MTT assay. The mRNA and protein levels of HIF-1α were accessed by RT-PCR and western blot, respectively. The radiosensitivity of U251 cells was detected by clonogenic assay. The data showed that the treatment by 100 µmol•L−1 CoCl2 and 0.5 µmol•L−1 WP1066 for 24 h had no cytotoxic effects on U251 cells (p>0.05). CoCl2 increased the expression of HIF-1α and clonogenic ability of U251 cells exposed to different doses of X-rays (p<0.05). Combined treatment by CoCl2 together with WP1066 suppressed the expression of HIF-1α, thus decreased the clonogenic survival fractions of U251 cells. The study reveals that WP1066 could enhance the radiosensitivity of U251 glioma cells under hypoxia conditions. The mechanism might be related to the downregulation of HIF-1α expression.Cited

关键词

氯化钴WP1066HIF-1αSTAT3脑胶质瘤细胞放射敏感性

Keywords

CoCl2WP1066HIF-1αSTAT3Glioma cellsRadiosensitivity

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Department of Radiation Oncology, The First Affiliate Hospital of Anhui University of Science and Technology

Department of Cell Biology, School of Medicine, Anhui University of Science and Technology

First Clinical Hospital, School of Medicine, Anhui University of Science and Technology

Department of Radiation Oncology, the Fourth Affiliated Hospital of China Medical University

苏州大学医学部放射医学与防护学院/放射医学及交叉学科研究院

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DOI：10.11889/j.1000-3436.2014.rrj.32.010201

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关键词

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