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WANG Weipeng; SUN Jing; CHEN Yuzhi; LI Bingyan; TONG Jian

doi:10.11889/j.1000-3436.2014.rrj.32.010204

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更新时间：2021-12-01

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- Journal of Radiation Research and Radiation Processing Vol. 32, Issue 1, Pages: 10204-010204(2014)
- 作者机构：
  
  1. （苏州大学医学部放射医学与公共卫生学院卫生毒理教研室）,苏州,215123
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- DOI：10.11889/j.1000-3436.2014.rrj.32.010204
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WANG Weipeng, SUN Jing, CHEN Yuzhi, et al. [J]. Journal of Radiation Research and Radiation Processing 32(1):10204-010204(2014)
DOI：

WANG Weipeng, SUN Jing, CHEN Yuzhi, et al. [J]. Journal of Radiation Research and Radiation Processing 32(1):10204-010204(2014) DOI： 10.11889/j.1000-3436.2014.rrj.32.010204.

摘要

使用溴化乙锭(Ethidium bromide, EtBr)诱导方法构建线粒体数目减少支气管上皮细胞(Human bronchia epithelia with mitochondrial DNA knock-down, ρ?HBE)模型并进行长期氡照射，用克隆形成法测定氡照射后HBE细胞的增殖能力，用流式细胞仪进行细胞凋亡和线粒体膜电位的检测。结果发现，氡照射后，与线粒体DNA数量正常的HBE细胞(ρ+ HBE)相比较，ρ?HBE细胞存活分数明显增高，虽然早期凋亡率明显低于正常细胞，但是总凋亡率增加，同时线粒体膜电位也显著降低。结果提示，氡照射后引起的线粒体减少HBE细胞增殖能力提高与总凋亡率的减少有关，并且与线粒体膜电位的变化相关。

Abstract

Human bronchia epithelia with mtDNA decrease (ρ- HBE) cells generated by treatment of ethidium bromide (EB) were exposed to radon gas in a special inhalation chamber. Cell proliferation was determined by cell survival assay. Cell apoptosis and membrane potential of mitochondria were analyzed by flow cytometry. The results showed that the survival fraction of ρ? HBE cells significantly increased compared with that of ρ+ HBE cells after irradiation with radon and its progeny. Although the apoptosis rate of ρ- HBE cells was lower than that of the ρ+ HBE cells at early period, the total apoptosis rate was increased, along with the membrane potential decrease of mitochondria in ρ? HBE cells. The results indicate that the increased potential of ρ? HBE proliferation correlates with the total apoptotic rate and mitochondrial membrane potential.Cited

关键词

线粒体DNA氡及其子体人支气管上皮细胞凋亡细胞增殖

Keywords

Mitochondrial DNARadon and its progenyHuman bronchial epithelial cellsApoptosisCell proliferation

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DOI：10.11889/j.1000-3436.2014.rrj.32.010204

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