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JIA Sisi1; LIANG Le1; DENG Suhui1; HOU Shangguo1; LIU Huajie1; HUANG Qing1; FAN Chunhai1; 2

doi:10.11889/j.1000-3436.2014.rrj.32.040101

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- Journal of Radiation Research and Radiation Processing Vol. 32, Issue 4, Pages: 40101-040101(2014)
- 作者机构：
  
  1. 1（中国科学院上海应用物理研究所物理生物学室嘉定园区）,上海,201800
  2. 2（中国科学院上海应用物理研究所光源生物成像中心嘉定园区）,上海,201800
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- DOI：10.11889/j.1000-3436.2014.rrj.32.040101
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JIA Sisi1, LIANG Le1, DENG Suhui1, et al. [J]. Journal of Radiation Research and Radiation Processing 32(4):40101-040101(2014)
DOI：

JIA Sisi1, LIANG Le1, DENG Suhui1, et al. [J]. Journal of Radiation Research and Radiation Processing 32(4):40101-040101(2014) DOI： 10.11889/j.1000-3436.2014.rrj.32.040101.

摘要

本文介绍了3种常用的亚细胞结构成像技术包括荧光超分辨显微成像术、软X-射线显微镜和三维冷冻电镜的成像原理，细胞成像应用及所存在的问题。并提出多种显微镜联用的关联显微术由于结合了多种显微镜的优势，能够提供多方位信息，丰富细胞生物学的研究，有可能给细胞学带来全新的认识。

Abstract

This review focused on the developments of three common microscopes in cell biology including far-field light nanoscopy, soft X-ray microscopy based on the synchrotron radiation light source, and three-dimensional cryo-electron microscopy. Their mechanisms, applications in cell imaging and the existing problems are discussed. The correlative microscopy combining several imaging methods can supply more information and may provide a new insight into cell biology in the future.Cited

关键词

亚细胞显微成像荧光超分辨显微成像术软X-射线显微镜三维冷冻电镜关联显微术

Keywords

Subcellular imagingFluorescence super-resolution microscopySoft X-ray microscopyThree- dimensionalcryo-electronmicroscopyCorrelative microscopy

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DOI：10.11889/j.1000-3436.2014.rrj.32.040101

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