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XIA Ying1; WANG Ye1; XUE Lian2; YU Dong1

doi:10.11889/j.1000-3436.2014.rrj.32.040201

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更新时间：2021-12-01

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- Journal of Radiation Research and Radiation Processing Vol. 32, Issue 4, Pages: 40201-040201(2014)
- 作者机构：
  
  1. 1（苏州大学放射医学与防护学院）,苏州,215123
  2. 2（苏州大学公共卫生学院）,苏州,215123
- 作者简介：
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- DOI：10.11889/j.1000-3436.2014.rrj.32.040201
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XIA Ying1, WANG Ye1, XUE Lian2, et al. [J]. Journal of Radiation Research and Radiation Processing 32(4):40201-040201(2014)
DOI：

XIA Ying1, WANG Ye1, XUE Lian2, et al. [J]. Journal of Radiation Research and Radiation Processing 32(4):40201-040201(2014) DOI： 10.11889/j.1000-3436.2014.rrj.32.040201.

摘要

采用克隆形成实验检测U0126对A549细胞的放射增敏作用；MTT法检测U0126联合X-射线对A549细胞的增殖抑制作用；流式细胞术检测U0126联合X-射线对A549细胞周期的影响；蛋白免疫印迹杂交法检测p-erk蛋白在A549细胞中的表达；衰老相关β-半乳糖苷酶染色检测A549细胞衰老的变化。结果显示，U0126使p-erk蛋白表达下降，能够增加A549细胞的放射敏感性，放射增敏比为1.18。单纯使用U0126能明显抑制A549细胞的增殖，诱导G1期细胞阻滞并具有时间依赖性和剂量依赖性。U0126联合X-射线也能抑制A549细胞的增殖，增强G1期细胞阻滞现象和细胞衰老现象。以上结果表明，U0126能增加A549细胞的放射敏感性，其作用机制可能与细胞周期阻滞及细胞衰老增强有关。

Abstract

The radiosensitizing effects of MEK inhibitor U0126 on A549 cells were determined by colony forming assay. A549 cells were exposed to X-ray with or without U0126 treatment. MTT assay was used to evaluate the cell viability. Cell cycle distribution was analyzed by flow cytometry. Expression of p-erk protein was confirmed by Western Blot. SA-β-gal assay was used to measure the phenomenon of senescence. The results showed that U0126 could decrease the expression of p-erk protein while enhance the radiosensitivity of A549 cells, and the SER was 1.18. ERK inhibition significantly suppressed cell viability and induced G1-phase arrest. After treatment of U0126 combined with X-ray irradiation, the phenomenon of G1-phase arrest and senescence appeared more obvious. U0126 can enhance the radiosensitivity of A549 cells. The radiosensitizing effects of U0126 on A549 cells may contribute to the increase in phenomenon of G1-phase arrest and senescence.Cited

关键词

抑制剂U0126A549细胞放射增敏细胞周期阻滞衰老

Keywords

MEK inhibitor U0126A549 cellsRadiosensitizationCell cycle arrestSenescence

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DOI：10.11889/j.1000-3436.2014.rrj.32.040201

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