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OUYANG Kehan1; SHEN Xianrong1; 2; HE Ying2

doi:10.11889/j.1000-3436.2014.rrj.32.040204

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更新时间：2021-12-01

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- Journal of Radiation Research and Radiation Processing Vol. 32, Issue 4, Pages: 40204-040204(2014)
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- DOI：10.11889/j.1000-3436.2014.rrj.32.040204
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OUYANG Kehan1, SHEN Xianrong1, 2, et al. [J]. Journal of Radiation Research and Radiation Processing 32(4):40204-040204(2014)
DOI：

OUYANG Kehan1, SHEN Xianrong1, 2, et al. [J]. Journal of Radiation Research and Radiation Processing 32(4):40204-040204(2014) DOI： 10.11889/j.1000-3436.2014.rrj.32.040204.

摘要

将60只大鼠随机分成4组,即照射14 d的混合照射组20只、14 d的空白对照组10只、照射31 d的混合照射组20只、31 d的空白对照组10只。照射组每天用低剂量率60Co γ-射线(0.0167 Gy•h−1)照射2 h和252Cf中子(0.028 mGy•h−1)照射20 h，在照射14 d、31 d后，各处死20只受照大鼠及10只对照大鼠，提取肝脏总RNA，用实时荧光定量PCR技术检测肝脏中CYP7A1基因及参与调控该基因表达的核受体—PPARα(Peroxisome proliferator activated receptor ?)、FXR(Farnesoid X receptor)、PXR(Pregnane X receptor)、HNF4?(Hepatocyte nuclear factor 4?)和LXR?(Liver X receptor ?)的转录水平。结果表明：混合照射14 d后，大鼠肝细胞CYP7A1基因mRNA表达显著下调至空白对照组的44%(p<0.01)，PXR基因mRNA表达上调至空白对照组的1.69倍，PPAR?、FXR、HNF4?及LXR?基因 mRNA表达无明显变化；混合照射31 d后，大鼠肝细胞CYP7A1基因mRNA表达显著下调至空白对照组的22%(p<0.01)，PXR基因mRNA表达显著上调至空白对照组的2.34倍(p<0.01)，PPAR?、FXR、HNF4?及LXR?基因 mRNA表达无明显变化。一定时期内,低剂量率中子-γ射线混合照射导致大鼠肝细胞CYP7A1基因的表达随着受照剂量的增加而显著下降，其机制可能是混合照射上调了PXR基因的表达,而PXR对CYP7A1基因表达起抑制作用。

Abstract

The aim was to analyze the effects of low dose rate neutron-gamma mixed irradiation on the mRNA expression of hepatic cholesterol 7α-hydroxylase (CYP7A1) gene in the rat and its potential mechanism. Sixty rats were randomly divided into 4 groups: irradiated group in which 20 rats were irradiated for 14 d; control group in which 10 rats were sham irradiated for 14 d; irradiated group in which 20 rats were irradiated for 31 d; control group in which 10 rats were sham irradiated for 31 d. The irradiated rats were irradiated with low dose rate 60Co γ-rays at 0.0167 Gy•h−1 for 2 h and 252Cf neutron at 0.028 mGy•h−1 for 20 h. After being irradiated for 14 d and 31 d, 20 irradiated rats and 10 control rats were killed respectively. The total RNA was extracted from livers. The real-time PCR was used to detect the expression level of hepatic CYP7A1 gene and some nuclear receptor genes, such as peroxisome proliferator activated receptor alpha (PPARα), farnesoid X receptor (FXR), pregnane X receptor (PXR), hepatocyte nuclear factor 4 alphas (HNF4?) and liver X receptor alpha (LXR?), which involved in CYP7A1 transcriptional regulation. The results showed that the mRNA level of hepatic CYP7A1 gene in the irradiated rats which were irradiated for 14 d significantly decreased by 44%( p<0.01) of that in the control group rats, the mRNA level of hepatic PXR gene in the irradiated rats which were irradiated for 14 d increased by 1.69-fold of that in the control group rats, while the mRNA levels of PPARα, FXR, HNF4α and LXRα in the irradiated rats which were irradiated for 14 d did not change significantly compared with the control rats; the mRNA level of hepatic CYP7A1 gene in the irradiated rats which were irradiated for 31 d rather significantly decreased by 22%(p<0.01) of that in the control rats, the mRNA level of hepatic PXR gene in the irradiated rats which were irradiated for 31 d significantly increased by 2.34-fold (p<0.01) of that in the control rats, while the mRNA levels of PPARα, FXR, HNF4α and LXRα in the irradiated rats which were irradiated for 31 d didn’t change significantly compared with the control rats. Thus, after exposure to low dose rate neutron-γ rays co-irradiation in a period, the mRNA level of hepatic CYP7A1 gene in the rat significantly decreased with the accumulation of the absorbed dose. The possible mechanism of the change was that the co-irradiation led to the significant increase transcriptional level of PXR gene, but PXR repressed the transcription of CYP7A1 gene.Cited

关键词

低剂量率60Co γ-射线中子CYP7A1基因大鼠

Keywords

Low dose rate60Co γ-raysNeutronCYP7A1 geneRat

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Second Affiliated Hospital, Suzhou University

Electron Microscopic Center, Faculty of Preclinical Medicine,Fourth Military Medical University

Department of Radiation Medicine, Faculty of Military Prevention Medicine,Fourth Military Medical University

Institute of Radiation Medicine, Academy of Military Medical Sciences

Affiliated Hospital , Academy of Military Medical sciences

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DOI：10.11889/j.1000-3436.2014.rrj.32.040204

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