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WANG Zhidong1; SHEN Liping1; LIN Zhongwu2; ZHANG Xueqing1; LI Xiaoxin1; CHEN Ying1

doi:10.11889/j.1000-3436.2014.rrj.32.050205

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更新时间：2021-12-01

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- Journal of Radiation Research and Radiation Processing Vol. 32, Issue 5, Pages: 6-050205(2014)
- 作者机构：
  
  1. 1（军事医学科学院放射与辐射医学研究所）,北京,100850
  2. 2（军事医学科学院科技部）,北京,100850
- 作者简介：
- 基金信息：
- DOI：10.11889/j.1000-3436.2014.rrj.32.050205
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WANG Zhidong1, SHEN Liping1, LIN Zhongwu2, et al. [J]. Journal of Radiation Research and Radiation Processing 32(5):6-050205(2014)
DOI：

WANG Zhidong1, SHEN Liping1, LIN Zhongwu2, et al. [J]. Journal of Radiation Research and Radiation Processing 32(5):6-050205(2014) DOI： 10.11889/j.1000-3436.2014.rrj.32.050205.

摘要

以人外周血全血为研究对象，筛选本底值均一的辐射敏感基因，寻找辐射损伤早期的生物标志物。采集3名健康成人外周血，采用?-射线对其分别进行0、0.75、2和6 Gy照射，照射后2 h提取全血总RNA，利用全基因组芯片筛选差异表达基因，并分析差异表达基因在3个个体中的本底值。结果显示，共筛选到15个具有相对均一本底值的辐射诱导基因，这些基因照射后的变化具有一定的剂量依赖性：ISG20L1、TRAF4和TRIAP1等3个基因在0.75、2和6 Gy 3个剂量照射后2 h均上调2倍以上；LOC345537、POLH、SPIB、TNFSF9和TCL1A等5个基因在2 Gy和6 Gy照射后2 h变化2倍以上，其中TLC1A为下调表达，其余4个上调表达；C12orf5、DDB2、GADD45A、MADCAM1和RPS27L仅在6 Gy照射后2 h上调2倍以上。

Abstract

To screen radiation-sensitive genes with uniform background levels in human peripheral blood, which can be used as biomarkers for radiation injury, peripheral blood samples from three healthy adults were exposed to gamma-rays with dose of 0, 0.75, 2 and 6 Gy. The total RNA was extracted from the whole blood and differential gene expression profiling was analyzed using microarray at 2 h after irradiation. The results showed that 15 differential expressive genes with a relatively uniform background were found: ISG20L1, TRAF4 and TRIAP1 were up-regulated over 2-fold at 2 h after 0.75, 2 and 6 Gy irradiation; LOC345537, POLH, SPIB and TNFSF9 were up-regulated over 2-fold, and TCL1A was down-regulated over 2-fold at 2 h after 2 and 6 Gy irradiation; C12orf5, DDB2, GADD45A, MADCAM1 and RPS27L were up-regulated over 2-fold at 2 h after 6 Gy irradiation.Cited

关键词

电离辐射基因芯片辐射敏感基因生物标志物

Keywords

Ionizing radiationMicroassayRadiation-sensitive genesBiomarkers

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Key Laboratory of Space Radiobiology of Gansu Province，Institute of Modern Physics， Chinese Academy of Sciences

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DOI：10.11889/j.1000-3436.2014.rrj.32.050205

摘要

Abstract

关键词

Keywords

references