暂无标题

XING Xiaomeng; WANG Yan; DU Liqing; XU Chang; LIN Kaili; FAN Saijun; LIU Qiang

doi:10.11889/j.1000-3436.2014.rrj.32.060203

您当前的位置：

首页 >

文章列表页 >

暂无标题

更新时间：2021-12-01

- 暂无标题
- Journal of Radiation Research and Radiation Processing Vol. 32, Issue 6, Pages: 4-060203(2014)
- 作者机构：
  
  1. （北京协和医学院中国医学科学院放射医学研究所）,天津,300192
- 作者简介：
- 基金信息：
- DOI：10.11889/j.1000-3436.2014.rrj.32.060203
  CLC：
扫描看全文
XING Xiaomeng, WANG Yan, DU Liqing, et al. [J]. Journal of Radiation Research and Radiation Processing 32(6):4-060203(2014)
DOI：

XING Xiaomeng, WANG Yan, DU Liqing, et al. [J]. Journal of Radiation Research and Radiation Processing 32(6):4-060203(2014) DOI： 10.11889/j.1000-3436.2014.rrj.32.060203.

摘要

采用MTT法检测不同浓度白藜芦醇对肺癌A549细胞的毒性；克隆形成实验测定白藜芦醇对A549细胞增殖抑制作用；碱性单细胞凝胶电泳实验（彗星实验）检测细胞DNA受损情况；采用Western blot法检测白藜芦醇与辐射联合作用对抑制凋亡蛋白Survivin的影响。结果表明，白藜芦醇对肺癌A549细胞的抑制作用随着白藜芦醇浓度升高及时间延长而增加；白藜芦醇与辐射联合作用可以明显增加辐射所致A549细胞的DNA损伤(p<0.05)，并降低克隆形成率及抑制凋亡蛋白Survivin的表达。表明白藜芦醇对A549细胞具有放射增敏作用，且放射增敏作用可能是通过抑制Survivin蛋白的表达实现的。

Abstract

We aim to investigate the effect and mechanism of resveratrol on radiosensitivity of lung cancer A549 cells. MTT was used to detect the cell toxicity of different concentrations of resveratrol on lung cancer A549 cells; clonogenic assay was performed to determine the function of growth inhibition. The DNA damage was detected by the comet assay. Western blot was taken to detect the expression level of Survivin. The results showed that the inhibitory effect of resveratrol on A549 cells increased with resveratrol concentration and time; the combined effect of resveratrol and radiation significantly increased the DNA damage of A549 cells induced by radiation(p<0.05), and reduced cloning efficiency as well as the expression level of Survivin. It can be concluded that resveratrol can enhance the radiation sensitivity of A549 cells, and maybe the radiosensitizing effect is achieved by suppressing the expression of Survivin protein.Cited

关键词

白藜芦醇肺癌A549细胞辐射增敏Survivin 蛋白

Keywords

ResveratrolLung cancer A549 cellsRadiosensitizationSurvivin protein

references

查看原文

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Protective effect of resveratrol on UVA-irradiated HaCaT cells and its mechanism

Syntheses and radiosensitization effect of mitochondria-targeted 6-(4-(dimethylamine) pyridine) methyl coumarin

Effect of LGK-974 in enhancing the radiosensitivity of HepG2 hepatoma cells

Radiation protection effects of Vam3 on mouse thymocyte in vitro

THE RADIOSENSITIZATION ACTIVITIES OF NITROTRIAZOLE DERIVATIVES AND QUINOXALINE di-N-OXIDES

Related Author

No data

Related Institution

National Institute for Radiological Protection, Key Laboratory of Radiological Protection and Nuclear Emergency, Chinese Center for Disease Control and Prevention

Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Peking Union Medical College & Chinese Academy of Medical Science

Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College

Institute of Laboratory Animal Sciences, CAMS & PUMC

Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences, Peking Union Medical College

⁰

暂无标题

DOI：10.11889/j.1000-3436.2014.rrj.32.060203

摘要

Abstract

关键词

Keywords

references