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HE Ying; SHEN Xianrong; QIAN Tiantian; WANG Qingrong; JIANG Dingwen; CHEN Wei

doi:10.11889/j.1000-3436.2015.rrj.33.010202

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更新时间：2021-12-01

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- Journal of Radiation Research and Radiation Processing Vol. 33, Issue 1, Pages: 10202-010202(2015)
- 作者机构：
  
  1. （海军医学研究所）,上海,200433
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- DOI：10.11889/j.1000-3436.2015.rrj.33.010202
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HE Ying, SHEN Xianrong, QIAN Tiantian, et al. [J]. Journal of Radiation Research and Radiation Processing 33(1):10202-010202(2015)
DOI：

HE Ying, SHEN Xianrong, QIAN Tiantian, et al. [J]. Journal of Radiation Research and Radiation Processing 33(1):10202-010202(2015) DOI： 10.11889/j.1000-3436.2015.rrj.33.010202.

摘要

为观察低剂量γ-射线对人淋巴母细胞AHH1 B细胞转位基因2(B cell translocation gene 2, BTG2)基因mRNA表达水平的影响，探讨BTG2基因作为低剂量范围辐射生物剂量计的可能性，采用0−1.0 Gy γ-射线照射人淋巴母细胞，照射后不同时间点(0−72 h)提取细胞总 RNA，采用实时荧光定量PCR方法检测BTG2基因的表达变化，分析该基因的时间−剂量效应关系。结果表明，照射后BTG2基因转录水平表达在低剂量范围呈剂量依赖增加，照射后24 h达峰值，之后逐渐下降，在48 h和72 h时处于平台期，在168 h恢复至初始水平。BTG2基因对低剂量电离辐射敏感，有较好的剂量效应和时间效应关系，具有开发为低剂量辐射生物剂量计的潜力。

Abstract

The aim is to investigate the effects of low-dose γ-rays on gene expression of BTG2 and the probability of it as bio-dosimeter. AHH-1 lines were irradiated with ?-rays of 0−1.0 Gy. Total RNA of cells was extracted at 0−168 h after irradiation, and real-time quantitative PCR was used to detect BTG2 gene expression. The relationships between the dose or time and gene expression were analyzed. After irradiation, the BTG2 gene expression increased in a dose-dependent form. Its peak expression was at 24 h after irradiation, and then the BTG2 gene expression gradually decreased to platform stage at 48 h and 72 h, and finally returned to the level of 0 h at 168 h. BTG2 gene is sensitive to irradiation and has potential in usage as biological dosimeter for its good dose and time-effect relationships.Cited

关键词

低剂量电离辐射人淋巴母细胞BTG2基因表达实时荧光定量PCR

Keywords

Low-dose ionizing radiationHuman lymphoblastoid cellsBTG2Gene expressionReal-time quantitative PCR

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National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention

National Institute for Radiation Protection, Chinese Center for Disease Control and Prevention

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DOI：10.11889/j.1000-3436.2015.rrj.33.010202

摘要

Abstract

关键词

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