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LI Xiaoxin; HU Jiangwei; ZHANG Xueqing; SHEN Liping; LI Lin; CHANG Cheng; WANG Zhidong; CHEN Ying

doi:10.11889/j.1000-3436.2015.rrj.33.050205

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更新时间：2021-12-01

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- Journal of Radiation Research and Radiation Processing Vol. 33, Issue 5, Pages: 50205-050205(2015)
- 作者机构：
  
  1. 1（安徽医科大学）,合肥,230032
  2. 2（军事医学科学院放射与辐射医学研究所）,北京,100850
  3. 3（解放军307医院）,北京,100071
- 作者简介：
- 基金信息：
- DOI：10.11889/j.1000-3436.2015.rrj.33.050205
  CLC：
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LI Xiaoxin, HU Jiangwei, ZHANG Xueqing, et al. [J]. Journal of Radiation Research and Radiation Processing 33(5):50205-050205(2015)
DOI：

LI Xiaoxin, HU Jiangwei, ZHANG Xueqing, et al. [J]. Journal of Radiation Research and Radiation Processing 33(5):50205-050205(2015) DOI： 10.11889/j.1000-3436.2015.rrj.33.050205.

摘要

以人外周血为研究对象，研究照射后RPS27L基因的时间效应和剂量效应以及本底水平，探究其作为辐射损伤早期生物标志物的可行性。采集3名健康成人外周血，利用γ 射线对其进行照射至吸收剂量分别为0、0.5、1、2、4、6和10 Gy，受照后37 ℃培养2、4、8、12和24 h，利用实时荧光定量PCR检测RPS27L基因的表达变化，并分析在37个健康人个体中的本底水平。结果显示，受照后的4、8、12和24 h RPS27L基因表达变化显著，而且具有很好的剂量依赖关系；在不同年龄和性别的个体中RPS27L本底水平相对稳定。因此，RPS27L基因具有作为新的辐射损伤标志物的潜力。

Abstract

In order to explore RPS27L’s possibility as a biomarker of radiation damage, we studied its time-response and dose-response after irradiation as well as its background level. Three healthy adults’ peripheral blood was exposed to gamma-rays with 0, 0.5, 1, 2, 4, 6, and 10 Gy, separately and cultured for 2, 4, 8, 12, and 24 h respectively. RPS27L’s expression change was detected by quantitative real-time PCR. We also analyzed its background level in 37 individuals. The results showed that RPS27L had a significant difference at 4, 8, 12, 24 h and a great dose-independent response. There was also a uniform background level of RPS27L among different age and gender. Thus, RPS27L is of great potential to be a new biomarker of radiation.Cited

关键词

核辐射RPS27L基因表达变化实时荧光定量PCR生物标志物

Keywords

Nuclear radiationRPS27LGene expression changeQuantitative real-time PCRBiomarker

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Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Jiading Campus

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DOI：10.11889/j.1000-3436.2015.rrj.33.050205

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