The aim is to investigate the effects of 1950 MHz radiofrequency radiation (GSM-Talk Signal) on mouse sertoli cells proliferation and secretion. The adherent TM4 cells were cultured in DMEM/F12 1:1 containing 10% FBS. Exponentially growing cells were digested to cell suspension of 1.5 × 103 cells•mL−1 and then cultured in 35 mm petri dishes with 3 mL for each dish, 12 dishes in total. After incubation for 24 h, medium was changed and the dishes were randomly divided into sham-exposure group and exposure group (GSM-Talk signal, 1950 MHz continuous wave, the specific absorption rate was 3 W•kg−1) with 6 dishes for each group. The dishes were placed in exposure waveguide chamber and sham-exposure waveguide chamber. After exposure for 5 d, the proliferation of cells was evaluated by CCK-8 and BrdU methods. The expression of cell proliferation marker Ki67 was measured through immunofluorescence staining. The levels of mouse stem cell factor (SCF) and mouse glial cell line-derived neurotrophic factor (GDNF) in cell supernatants were detected by ELISA assay. The results showed that compared with sham-exposure group, the proliferation of TM4 cells in exposure group significantly decreased(p<0.05), and the Ki67 level also tended to be reduced; the level of SCF in cell supernatants was lower (p<0.05) while the level of GDNF was higher than that of sham-exposure group (p<0.05). The results suggest that the GSM-Talk signal exposure for 5 d could affect the proliferation and secretion of TM4 cells.Cited