最新刊期
      期 2 ， 2002
      • JIN Yizun
        Vol. 20, Issue 2, Pages: 81-86(2002)
        摘要：The current clinical statue of chemical modifiers of cancer treatment is described in this paper. It should enable greater enhancement ratios to be attained clinically. To search for less toxic and more potent sensitizers for radiotherpy and chemotherpy will be improved the cancer treatment. Phase Ⅰ, Ⅱ and Ⅲ clinical trials with TPZ,one of the bioreductive drugs, has shown the benefit to patients with cancer.  
        关键词：Cancer treatment;Chemical modifier;Clinic trial   
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        发布时间：2023-04-07
      • LIU Guanshu,YAO Side,NI Jin,ZHAO Fang,MENG Xiangshun
        Vol. 20, Issue 2, Pages: 87-92(2002)
        摘要：The radioprotecive effects of a major component of green tea polyphenone,(—)epigallocatechin gallate (EGCG) was examined by using the radiation — induced cell death model in Human — derived liver L02 cells,with the methods of MTT and pulsed field gel electrophoresis (PFGE). EGCG substantially suppressed the radiation — induced mortality under treatment concentration of 5—50μmol/L, where the cytotoxicity was mild. Significant suppression of DNA double strand break (DSB) was also observed with treatment of cells with 50μmol/L of EGCG. Both treatment prior to and behind irradiation was effective. It could be suggested that EGCG could either scavenge OH• or repair the damage of DNA of L02cells effectively.  
        关键词：Epigallocatechin gallate (EGCG);Human—derived liver L02 cell;Radio- protection;MTT;Pulsed field gel electrophoresis (PFGE)   
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        发布时间：2023-04-07
      • SONG Weiqiang,HU Weimin,ZHU Jun,ZHAO Huidong,YANG Mingcheng,LUO Jiquan
        Vol. 20, Issue 2, Pages: 98-102(2002)
        摘要：A new radiation vulcanization process of water - swellable rubber comprised of a chloroprene rubber and crosslinked polyacrylate,has been performed by radiation vulcanization. The results indicate that the water—swellable rubber vulcanized at 40kGy with the weight proportion of the chloroprene rubber to the polyacrylate sodium 4:3 has the best comprehensive properties.  
        关键词：Water—swellable rubber;γ Ray radiation vulcanization;Chloroprene rubber;Cross—linked polyacrylate sodium   
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        发布时间：2023-04-07
      • ZHANG Tongcheng,ZHONG Hongliang,LIU Qingfang,MI Zhisu,WANG Chunlei
        Vol. 20, Issue 2, Pages: 103-107(2002)
        摘要：Bioburdens on seven kinds of medical products were determined in accordance with ISO 11737. Different data were obtained for different products. The bioburden changed from 10 cfu/device to 97271 cfu/device the recovery ratio from 54.6% to 100%,and the correcting coefficiency from 1.0 to 1.8. Based on such bioburden and method 1 of ISO 11137,the verification dose was set up for each kind of products,from 5.1 kGy to 17. 6 kGy. 100 samples were taken respectively from each kind of products and exposed to the pre — defermined verificction dose. The sterility test showed the positive number was not more than 2. Thus the sterilication dose was determined according to Table B.1 of ISO11137.  
        关键词：Bioburden;Radiation sterilization;Verification dose;Sterility assurance level (SAL)   
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        发布时间：2023-04-07
      • HUANG Wei,LUO Shikai,FU Yibei,BIAN Zhishang,HE Meiying
        Vol. 20, Issue 2, Pages: 108-112(2002)
        摘要：In this paper,the thermal stability and mechanical properties of irradiated polyurethane foam have been studied by DTG and universal testing machine. And the gas products from irradiated samples were analyzed quantitatively and qualitatively by GC. The results show that the multiple mechanical properties are still good. But due to the action of radiation degradation, many gas products are released. The thermal property of material is stable after irradiation.  
        关键词：Polyether polyurethane;Radiation effect;Gas products from radiation degradation   
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        发布时间：2023-04-07
      • RAN Shuyang,FU Yuchuan,LUO Zhengming
        Vol. 20, Issue 2, Pages: 113-117(2002)
        摘要：The dose distribution in polyethylene and H2O were calculated by a new EGS4 version—EGSnrc Monte Carlo code system with 1.5MeV—3MeV initial electron energy. The effects of different metal base—plate such as Al、Cu and Pb for dose depth profile in materials were compared. And the effects of different electron beam size on absorbed dose were calculated, too. The dose was given by absolute dose (Gy.cm2). The calculation uncertainty was controlled within 2%. This kind of work proposes a useful theoretical tool for optimizing technology of electron radiation processing.  
        关键词：Electron beam;Radiation processing;Dose distribution;Monte Carlo technology;EGSnrc system   
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        发布时间：2023-04-07
      • LI Wei,WANG Wenfeng,QIAN Suping,YAO Side
        Vol. 20, Issue 2, Pages: 118-122(2002)
        摘要：Stable temperature sensitive polymer nanometer gel was prepared by the soap—less emulsion polymerization, which initiated by light and the diameter was successful controlled less than 100nm. The reactive system were composed by the aqueous main monomer N—isopropylacrylamide and methylenebisacrylamide, MBA. The effects of system compose and conditions on the diameter were studied. The result indicated that under the CMC,the gel diameter will continue declining and be invariable finally with increase the emulsifier concentration. Higher poly dispersity and smaller particles can be got in the polymerization initiated by light compared to the therm initiate.  
        关键词：Soap—less emulsion polymerization;Microgel;Temperature sensitive;Light initiate   
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        发布时间：2023-04-07
      • LI Yeping,HU Zhenpeng,FANG Yuee,CHU Gaosheng
        Vol. 20, Issue 2, Pages: 123-128(2002)
        摘要：The influences of various factors on the preparation of chitosan—sodium acrylate superabsorbent were discussed,including the mass ratio of reactants, irradiation dose,irradiation dose rate and the concentration of N,N'—Methylene — bis — acrylamide et al. The water absorption and the water retention of the resin were measured. The results showed that the appropriate conditions should be controlled as follows: the mass ratio of acrylic acid to chitosan ≈6: 1,the degree of neutralization≈75%,the mass fraction of N,N'—Methylene—bis—acrylamide≈0.3%—0.5%, the irradiation dose dose≈90 Gy•min-1, and the irradiation dose≈15kGy. The distilled water absorption of the resin was about 400mL•g-1, the tap water absorption of the resin was about 150mL•g-1 and the NaCl (0.9%) absorption of the resin was about 50mL•g-1. The speed of water absorption was rapid and the water retention under pressure was good.  
        关键词：Chitosan;Sodium acrylate;Superabsorbent;γ ray irradiation   
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        发布时间：2023-04-07
      • WU Wei,QU Changmin,WU Daimin
        Vol. 20, Issue 2, Pages: 146-150(2002)
        摘要：To examine the efficiency and fidelity of rejoining of the double strand break(dsb) DNA of the irradiated mouse Waldeyeer s Ring Equivalent (WRE) cells. With lipofectamine or electroporation, the linearized plasmid pPMH16 with two marker genes (neo and gpt)were transferred into the WRE cells from irradiated mice. Through ordinal G418 screen and XHAYM screen, the rejoining efficiency of the broken gpt gene wes evaluated. The rejoining efficiency of gpt gene was 69.75% for control WRE cells, and 36.05% for the WRE cells gotten form the irradiated mice 4h after exposure to 2Gy γ irradiated and 21.50% 4h after exposure to 4Gy γ irradiated. The results showed that the fidelity of the rejoining of dsb of DNA for the irratiated mouse WRE cells was lower than that for control, and as the radiation dose increased,the fidelity decreased. Both the lipofectamine or electroporation could be successfully used in gene transfection for primary culture of mouse WRE cells. And the wrong repair of dsb of DNA in irradiated mouse WRE cells might be caused frequently.  
        关键词：Fidelity of DNA repair;Gene transferred;Waldeyer's—Ring Equivalent cells;Radiation injury   
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        发布时间：2023-04-07
      • YANG Zhenguo,WANG Dewen,SONG Liangwen,PENG Ruiyun,GAO Yabing,LI Yang,GU Qingyang,LIU Jie
        Vol. 20, Issue 2, Pages: 151-157(2002)
        摘要：To investigate the Effect of inhaled Nitric Oxide on the expression of related cytokines and mitogens in radiation pulmonary fibrosis. Pulmonary fibrosis model was made induced by irradiation with 30Gy 60Co γ rays on the whole thorax of Wistar rats. The NO inhalation started one day before (B and C groups) and one month (D and E groups) after radiation exposure respectively. The concentration of NO inhaled was 20μg•g-1ppm (B and D groups) and 10μg•g-1(C and E groups). Group A was not treated except for irradiation. The immunohistochemistry and in situ hybridization were examined in 1W,1M,3M, and 6M after radiation. In group A,the mRNA transcription of TGFβ and ET, and the protein expression of TGFβ, TNFα, ET and AII was increased significantly in group A after irradiation. In group B and C, the expression TGFβ, TNFα,ET and AII was higher than normal controls,but lower than that in other groups. TGFβ, TNFα and ET, AII are important factors in the formation of lung fibrosis,and NO has preventive and therapeutic effect through inhibiting the activities of these factors.  
        关键词：Nitric oxide;Radiation pulmonary fibrosis;Cytokines   
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        发布时间：2023-04-07
      • LI Xiaofeng,MA Lin,ZHANG Ming,CHI Baorong
        Vol. 20, Issue 2, Pages: 158-160(2002)
        摘要：In this study, the change of cGMP of pineal gland in mice were observed with low dose radiation(LDR). The time—course changes of cGMP were detected by radioimmunoassay after WBI with 75mGy X rays. It was shown that the levels of cGMP of pineal gland in mice began to decrease and reached its lowest peak at 18h (p<0.01) after WBI. and began to increase at 24h and reached its highest peak at 72h (p<0.05). These results indicated that WBI with LDR could reduce to the cGMP levels of pineal gland cells in mice.  
        关键词：Low dose radiation;Pineal gland in mice;cGMP   
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        发布时间：2023-04-07

        研究论文

      • LIN Nianyun,CHEN Yiping,TU Tiecheng,Dong Jirong
        Vol. 20, Issue 2, Pages: 93-97(2002)
        摘要：Connecting with the character of ESR spectra at 77K and calculated LU MOs,HOMOs the linear relationship between spin transfer efficiency and molar ratios of nucleotide in DNA to sinapic acid or sinapine provides quantitative evidence of long range electron transfer within mixed aggregates.  
        关键词：ESR;Radioprotection mechanism;Sinapic acid;Longrange charge transfer   
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        发布时间：2023-04-07
      • ZHOU Jianhua,SU Liaoyuan,TONG Jian,ZHU Minqing,XUE Lian
        Vol. 20, Issue 2, Pages: 129-136(2002)
        摘要：The relationships between irradiation and B7.1 costimulatory molecule expression of human tumor cells were studied to explore reason of tumor cell's immunogenicity enhanced. The effect of γ rays irradiation on B7.1 molecule expression in human tumor cells which was irradiated with 30,40, 50,60Gy γ rays was investigated. At 24, 48, 72, 96h after irradiation the change of B7.1 molecule was measured by flow cytometry （FCM） with immunofluorescence technique. B7.1m RNA was determined by reverse transcription polymerase chain reaction （RT-PCR）. The cytotoxicity of lymphocyte upon tumor target cell after exposure was measured by 3H-thymidine（3H-TdR） release assay. The results showed that after γ rays irradiation SMMC-7721 hepatoma,PC-12 pheochromacytoma, A-459 lung cancer cell lines express B7.1 costimulatory molecule. RT-PCR showed that expression of B7.1 molecule in SMMC7721 is resulted from B7.1mRNA expression. Furthermore,B7.1 molecule membrane expression after γ rays irradiation is shown to enhance the cytotoxicity of lymphocyte. The data could explain the enhanced immunogenicity of tumor cells after irradiation, and could lead to new immunotherapy protocols. The experiments suggested that γ rays irradiation could selectively induce human tumor cells to express B7.1 costimulatory molecules and enhance immunogenicity of tumor cell.  
        关键词：γ rays;Irradiation;Human tumor cell;B7.1 molecule;Costimulatory   
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        发布时间：2023-04-07
      • ZHOU Zhou,WANG Xiaohua,Igisu Hideki,LIN Yuan,LOU Shufen,Matsuoka Masato,CHENG Tianmin,YU Zhengping
        Vol. 20, Issue 2, Pages: 137-145(2002)
        摘要：Effects of γ irradiation on mitogen-activated protein kinases （MAPKs） were examined in IEC-6 cells. In response to γ irradiation, Phosphorylation of intracellular signal-regulated protein kinase （ERK） was not significantly altered. The levels of phosphorylated forms of c-Jun NH2-terminal kinase （JNK） and p38 MAPK increased at 30 min following exposure to 6Gy γ irradiation, while the cell viability was lowered significantly at 12h. On the other hand,no clear changes were found in the total protein levels of ERK,JNK and p38 MAPK. Chelation of intracellular Ca2+ suppressed γ irradiation induced JNK and p38 MAPK phosphorylation almost completely, but removal of external Ca2+ did not. Activation of p38MAPK, but not ERK,was correlated with γ irradiation induced apoptosis. The present results showed that γ irradiation is a potent activator of JNK and p38 MAPK,and Ca2+ mobilized from intracellular stores plays an important role for the activation of MAPKs and the induction of apoptosis in IEC-6 cells.  
        关键词：γ irradiation;ERK;JINK;p38MAPK;Intracellular Ca2+;IEC-6 cells   
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        发布时间：2023-04-07
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