最新刊期
      期 1 ， 2004
      • Yuan Chengling,Yu Zengliang
        Vol. 22, Issue 1, Pages: 1-7(2004)
        摘要：The interaction between low-energy ions and matter has been concerned rarely comparing to that of high-energy ions. It is even more unusual to find studies of the interaction of low-energy ions and complicated organisms. However, the discovery of bioeffects induced by ion beam implantation has opened a new branch in the field of ion beam applications in the life science—Low-energy heavy ion biology. The mutagenic effect of low energy heavy ions was firstly reported in 1986 in rice. Since then, a damage mechanism involved in energy absorption, mass deposition, and charge exchange has been proposed. Accumulating evidence has indicated that these three factors are key determinants in the bioeffects induced by low energy heavy ions, which has opened new opportunities for mutational breeding, gene transferring, cell modification, and cell fusion. In recent years, the ion beam implantation technique has been widely applied in many fields, and increasing research interest in the field has been seen. In this paper, we summarize recent advances in research on the role of low-energy ions interms of the mechanisms and applications.  
        关键词：Low-Energy Heavy Ion Biology;ion beam;breeding technique;Radiation   
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        发布时间：2023-03-30
      • YANG Ruiyuan,WANG Min,SHEN Zhongqun,WANG Wenfeng
        Vol. 22, Issue 1, Pages: 8-12(2004)
        摘要：The degradation of methyl orange has been studied under 60Co γ-ray irradiation. results of the experiments show that the dye has gone a high rate of decolorization with high COD removal efficiency. The experiments show the feasibility of using γ–irradiation processing to treat the dye wastewater.  
        关键词：γ–irradiation;Degradation;COD;Methyl orange   
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        发布时间：2023-03-30
      • LIU Shiheng,WANG Shilong,SUN Xiaoyu,LI Wenzhe,NI Yaming,WANG Wenfeng,WANG Min,YAO Side
        Vol. 22, Issue 1, Pages: 13-18(2004)
        摘要：The kinetics and mechanisms of the reactions of 2-chlorophenol with hydroxyl radical, azide radical, hydrogen atom and hydrated electron have been studied using pulse radiolysis. In neutral solutions, relatively stable hexadienyl type OH-adducts is formed upon the addition of hydroxyl radical to the aryl ring of the substrate. In alkaline solutions, 2-chlorophenoxyl radical and semi-quinone radical anion are formed through the electron transfer from the substrate to hydroxyl radical as well as OH-addition followed by HCl/OH- elimination. Azide radical oxidizes 2-chlorophenol via one-electron transfer, forming correspondent o-chloro-phenoxyl radical. Hydrogen atom reacts with the substrate via the addition to the aryl ring, forming hexadienyl type •H-adducts. Under the attack of hydrated electron, 2-chlorophenol undergoes reductive Cl- elimination, with the rate constant of 3.5×108dm3•mol-1•s-1in alkaline solution and 2.2×109dm3•mol-1•s-1in neutral solution respectively. These results shed light on the degradation mechanism of chlorophenols and provide theoretical foundation for treatments of wastewater contaminated with chlorophenols.  
        关键词：2-Chlorophenol;Pulse radiolysis;Transient absorption spectra;Free radical   
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        发布时间：2023-03-30
      • SUN Fang,HUANG Yuli,XIA Lanying
        Vol. 22, Issue 1, Pages: 19-22(2004)
        摘要：Cationic photoinitiator(SR-1012) with Irgacure651 and Irgacure1700 were mixed, respectively. The influence of the content and composition of the composite photoinitiator on photosensitivity of the hybrid system composed of PU, triethyleneglycol diacrylic ester (TEGDA) and epoxy compound EPON-812 was studied. The results showed that the hybrid system had higher curing rate when using composite photoinitiator composed of Irgacure1700 and SR-1012 than that of Irgacure651 and SR-1012 and value of sensitivity was 2.64mJ/cm2. The cooperation of free radical initiator and SR-1012 offered considerable photosensitive properties, such as curing rate, sensitivity and gel yield, when the weight ratio of free radical initiator to SR-1012 was 2:1 and value of sensitivity was 6.28mJ/cm2. It was also found that suitable concentration of the composite initiator was 4-6%(wt). UV spectrum showed absorbance of λ at 276nm and 333nm.  
        关键词：Hybrid polymerization;UV-curing;Polyurethane acrylate   
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        发布时间：2023-03-30
      • ZHOU Chunlin,LI Tiantuo,MA Wenyan
        Vol. 22, Issue 1, Pages: 23-26(2004)
        摘要：This paper reports investigation results on natural radioactive nuclide contents of facing tile products from Xi’An construction market, and the products has been classified according to the national standard. The results show that natural radioactive nuclide contents in sampled facing tile products are in normal radioactive background level. The radio-activity ranges of U-238, Ra-226, Th-232 and K-40 are 17.6—82.5, 24.1—182, 13.4—75.7, 92.1—506Bq•kg-1, respectively. According to National Standard JC518-93, these tiles belong to A grade products and applications of the products shall not be limited.  
        关键词：γ-ray spectrum method;Facing tile;Natural radioactive nuclide   
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        发布时间：2023-03-30
      • LIU Ruiqin,WANG Huiyong,XIE Leidong,HOU Zhengchi,LI Linfan,YU Ming,YAO Side,SHENG Kanglong
        Vol. 22, Issue 1, Pages: 27-31(2004)
        摘要：Silk fabric samples were grafted with 2-hydroxypropyl acrylate monomer by ultraviolet rays, and a light-initiated graft copolymerization system without any additives or co-additives has been established. Experiments were performed to study the effects of monomer concentration, irradiation time, pH value, and reaction temperature on the grafting rate. FT-IR and DSC characterizations of the grafted silk samples were carried out to study the property changes in the silk fabrics. With advantages of fairly high graft rate, simpler equipment and operation, free of additives, and less homopolymer, the method can be an effective technique to improve the properties of silk fabrics.  
        关键词：Silk;Graft copolymerization;UV-irradiation;2-hydroxypropylacrylate   
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        发布时间：2023-03-30
      • Chen Qinglong,Chen Zhijun
        Vol. 22, Issue 1, Pages: 32-34(2004)
        摘要：The pile-up gamma irradiation techniques for decontamination of tea were conducted using dosimetry measurement of the product cartons irradiated The results indicated that the irradiation could be done with an overdose factor of less than 2 by position changes of the product cartons from the front to the back and from the top to the bottom. Dose effects on the total tea polyphenols and the total amino acids in the irradiated tea were studied. It was found that the optimal irradiation dose for decontamination of the tea was inbetween 3—6kGy.  
        关键词：Tea;Decontamination;γ-ray irradiation   
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        发布时间：2023-03-30
      • LI Xiujuan,LI Zhiguo,TIAN Mei,YANG Wei,LIU Linlin,LI Xiuyi
        Vol. 22, Issue 1, Pages: 35-38(2004)
        摘要：To study the regularity of mouse endostatin expression in B16 cells transfected by pEgr−ssEndostatin after different doses of X-ray irradiation and different time after the irradiation, mouse endostatin cDNA was amplified by RT−PCR and signal peptide was ligated to it. The pEgr−ssEndostatin recombinant plasmid was constructed and transfected into B16 cells with liposome and cells were irradiated. The expression of endostatin in the supernatant medium of B16 cells was detected by ELISA. The results showed that the cDNA sequence of mouse secretable endostatin is identical to the literatures. Egr−1 promoter and secretable endostatin cDNA were inserted into expression vectors correctly. pEgr−ssEndostatin had the expression property induced by radiation. It suggests that the Egr−1 promoter can be activated by ionizing irradiation and expression of downstream gene can be regulated.  
        关键词：Mouse endostatin cDNA;Egr−1 promoter;Recombinant plasmid of pEgr−ssEndostatin expression induced by radiation   
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        发布时间：2023-03-30
      • GU Qingyang,WANG Dewen,WANG Lisheng,DENG Hua,LIU Jie,PENG Ruiyun,DONG Bo,GAO Yabing,WANG Xiaomin
        Vol. 22, Issue 1, Pages: 39-42(2004)
        摘要：In order to investigate the effect of bFGF on radiation-induced apoptosis in vascular endothelial cells and its signal transduction pathways, ECV304 cells were irradiated with 60Co γ rays for establishing cell apoptosis model. Flow cytometry with Annexin-V-FITC-PI labeling was used to determine cell apoptosis. Different amounts of bFGF and MEK inhibitor U0126 were used to study their effects on radiation-induced endothelial cell apoptosis. It was found that bFGF could inhibit radiation-induced apoptosis in ECV304 cells, but U0126 could block the anti-apoptosis effect of bFGF, suggesting that bFGF may inhibit radiation-induced apoptosis in vascular endothelial cells through of MAPK signaling.  
        关键词：bFGF;vascular endothelial cells;Apoptosis;MEK;U0126   
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        发布时间：2023-03-30
      • Wang Xiaomei,Ju Guizhi,Fu Haiqing,Mei shujiang,Liu Shuzheng
        Vol. 22, Issue 1, Pages: 43-46(2004)
        摘要：To explore the molecular mechanism of G2 arrest of EL-4 cells induced by X-irradiation, flow cytometry (FCM) and semi-quantity RT-PCR methods were used to observe the time-course changes of cell cycle and the mRNA levels of cyclin B1 and cdc2 of EL-4 cells after 4Gy X-rays irradiation. Results showed that the positive cells in G2/M increased at 2h and reached the maximum at 4—8h after the irradiation (p<0.001). The cyclin B1 mRNA level of El-4 cells began decrease at 1h, reached the minimum at 24h after the irradiation. Meanwhile, the cdc2 mRNA level also declined during 12h to 48h, reduced to 59.2 %and 58.9% compared with that of control group respectively, and recovered at 72h after the irradiation. The results suggests that MPF(M phase promoting factor) activity reduction resulted from inhibition of cdc2 and cyclin B play a critical role in G2 arrest induced by X-ray irradiation in EL-4 cells.  
        关键词：Ionizing radiation;EL-4 cells;cyclin B;cdc2   
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        发布时间：2023-03-30
      • CHEN Xiaojing,JIN Yizun,SHEN Zhifen,TONG Shungao
        Vol. 22, Issue 1, Pages: 47-50(2004)
        摘要：The object of this work is to study the radiation sensitizing effect of suifonamide imizole in vitro and in vivo. In vitro radiosensitizing ability was estimated using V79 cells under hypoxia condition. Results show that SIM has radiosensitizing effect in vitro on Chinese hamster V79 cells and the SER value ranged from 1.12 to 1.81. In vivo radiosensitizing ability was estimated using C57BL mice bearing Lewis lung cancer. Different concentrations of drug were administered i.p. and local irradiation of 10Gy with 137Csγ-ray was given at 2h after administration. Tumor growth was observed for 20days and the tumor volume was measured every 2days. Results show that the tumor growth was suppressed after the treatment in the irradiated groups with SIM. The study indicates that SIM is a novel radiosensitizer.  
        关键词：suifonamide imidazole;Radiosensitization;in vitro;in vivo   
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        发布时间：2023-03-30
      • DING Weimin,TIAN Jiahe,ZHANG Jinming,FENG Huiru,HE Yijie,SHAO Mingzhe
        Vol. 22, Issue 1, Pages: 51-55(2004)
        摘要：HeLa cells were irradiated with 32P radioactive filter papers. Cells expressing senescence associated β-galactosidase(SA-β-Gal) were quantified. And to acquire information about the absolute numbers of SA-β-Gal+ cells and their changing tendency, the rates of cells expressing SA-β-Gal were corrected with cell growth curves. Results show that after low-dose-rate β irradiation, some tumor cells can reverse into mortality stage M1 or M2 cells which express SA-β-Gal. Numbers of SA-β-Gal+ cells induced by the irradiation were increased. And in dose rates of 7.5—60cGy/h, more SA-β-Gal+ cells could be induced by the irradiation than higher dose-rate irradiations at the same dose. Moreover, it may indicate that long-term therapeutic effect of internal irradiations could be resulted by induced aging of tumor cells .  
        关键词：β-rays;low-dose-rate;cell aging;beta-galactosidase;HeLa cells   
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        发布时间：2023-03-30
      • Li Hongyu,Li Chenghua,Ding Xinchun,Wang Jufang,Zhou Guangming,Xie Hongmei,Li Qiang,Dang Bingrong,Wen Xiaoqiong,Li Wenjian,Wei Zengquan
        Vol. 22, Issue 1, Pages: 56-60(2004)
        摘要：In this paper, red yeast (Rhodotorula RY Strain) that produces carotene is irradiated by 50Mev/u12C6+ heavy ion from Heavy Ion Accelerator in IMP. Fermentation tests show that 50Mev/u12C6+ heavy ion has a mutagenesis effect on the red yeast. some strains of red yeast with changed production of carotene were found by screening. Meanwhile, by RFLP and RAPD analysis, we have a further evidence that heavy ion can cause mutagenesis in Rhodotorula RY Strain. This presents a new prospect for the mutagenesis breeding by heavy ion in industry.  
        关键词：Heavy ion;Rhodotorula RY Strain;Mutagenesis effect;RFLP;RAPD   
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        发布时间：2023-03-30
      • XIE Hongmei,HAO Jifang,WEI Zengquan,XIE Zhongkui,LI Fengqin,WANG Yajun
        Vol. 22, Issue 1, Pages: 61-64(2004)
        摘要：Herbage seeds of legume and grass were irradiated in penetration by 80 MeV/u 20Ne10+ ions. The results of field tests and observations of the root−tip cells showed that growth of the seedlings was obviously weakened with increasing doses. Frequencies of chromosomal aberration and micronucleus increased significantly with increasing doses. According to the field growth tests, radiation sensitivity of grass herbage to the heavy ion beams was much higher than leguminous herbage, and suitable dose of the heavy ion irradiation for the grass and leguminous herbages is 20—30Gy and 150Gy, respectively.  
        关键词：Heavy ion beam;Herbage   
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        发布时间：2023-03-30
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