最新刊期
      期 3 ， 2006
      • GUO Yuefeng
        Vol. 24, Issue 3, Pages: 129-132(2006)
        摘要：The paper gives a review on features of telomeres with particular emphasis on a correlation between telomeres and organism sensitivity to ionizing radiations and their potential importance in radiation biology. Recent findings show that radiosensitivity can be impacted by changes in telomere length or telomere-being locations. Shortened and dysfunctional telomeres join to DNA breaks interfering with their correct repair. Chromosome instability resulting from telomere loss plays a significant role in chromosomal rearrangements associated with tumor progression. Telomere function is one of the factors that determine radiosensitivity of cells and whole organism.  
        关键词：Telomeres;Radiosensitivity   
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        发布时间：2023-03-30
      • YU Jiahua,LIU Fenju
        Vol. 24, Issue 3, Pages: 133-136(2006)
        摘要：EGFR belongs to tyrosine kinase receptors, and is overexpressed and mutant in a number of malignant tumors originated from epithelial origin. After ligand binding and activation, EGFR leads to a series of downstream signal pathways of activation and many biological effects. EGFR signal pathway is associated with radioresistance of tumor cells, and the mechanism is related to cell proliferation, reducing apoptosis, regulating cell cycle arrest, and DNA radiation damage repair. EGFR monoclonal antibodies and small molecule tyrosine kinase inhibitors can promote radiosensitization of tumor cells.  
        关键词：Epidermal growth factor receptor;Malignant tumor;Radiosensitization   
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        发布时间：2023-03-30
      • CHEN Jiwu,HU Bin,QIN Haiyan,AO Junping,ZHANG Jun,ZHU Zhenqin
        Vol. 24, Issue 3, Pages: 137-140(2006)
        摘要：·OH reacts with carbonate to yield carbonate radical anion (CO3·−) and chemiluminescence. Based on this reaction, chemiluminescence behavior of the system was investigated. Experimental conditions for the method were optimized. A chemiluminescence system for determination of CO3·− was developed. It was composed of 2mmol/L CuCl solution 80μL, 6.5mmol/L H2O2 solution 110μL and 75mmol/L carbonate buffer 810μL (pH9.8). The volume of reaction was 1.0mL. This system was characterized by its simplicity, rapidity, sensitivity and inexpensiveness. The recommended method had been successfully applied to determination of the ability of polyphenol, rutin, and quercetin scavenging CO3·− with satisfactory results.  
        关键词：Carbonate radical anion;Chemiluminescence system;Free radical scavenger   
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        发布时间：2023-03-30
      • WANG Huiliang,CHEN Wenxiu
        Vol. 24, Issue 3, Pages: 145-150(2006)
        摘要：Nitroxyl free radicals formed from several low molecular weight (LMW) hindered amine light stabilizers (HALS) under 60Co γ-ray irradiation was studied with electron spin resonance (ESR) spectroscopy. All the HALSs irradiated in air formed nitroxyl free radicals under irradiation in air. For most of the HALSs, concentration of the nitroxyl free radicals increased linearly and quickly with absorbed dose in 0-10kGy range, but increased slowly, or even kept constant, with doses of greater than 10kGy. Concentration of nitroxyl free radicals formed from LMW HALS was usually higher than high molecular weight HALS. Tetramethyl HALS was easier to form nitroxyl free radicals than pentamethyl HLAS. Concentration of nitroxyl free radicals formed from the samples irradiated in oxygen was about two times higher than that the samples irradiated in air. Mechanisms of the nitroxyl free radical formation from the γ-ray irradiated HALSs were was discussed.  
        关键词：Hindered amine light stabilizer (HALS);γ-ray irradiation;Nitroxyl free radical   
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        发布时间：2023-03-30
      • CHEN Yongkang,ZHOU Ruimin,ZHANG Xueping,RAO Weihong,HAO Xufeng,ZHOU Fei
        Vol. 24, Issue 3, Pages: 151-154(2006)
        摘要：Cadmium sulfide nano-particles have been successfully prepared by electro beam irradiation in the water system. The products were characterized by X-ray diffraction, transmission electron microscope and fluorescence spectroscopy. The results indicated that the CdS nano-particles were of pure face-centered cubic crystal and in spherical shape of, averaged about 15nm in size. The UV absorption edge of CdS nano-particles is 487nm. When the excitation wavelength was 350nm, the peak of the emission wavelength of CdS nano-particles is at 476nm, and the fluorescence was attributed to the emission of band edge. The influence of the concentration of PVA on the size of the CdS nano-particles was studied by laser scattering particle size distribution analysis. The results showed that the size of the CdS nano-particles decreased with increasing the concentration of polyvinyl alcohol during a certain extent.  
        关键词：EB-irradiation;CdS;Particle size;Nano-particals   
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        发布时间：2023-03-30
      • ZHU Jun,YANG Mingcheng,CHEN Haijun,LI Aimei
        Vol. 24, Issue 3, Pages: 155-158(2006)
        摘要：Dose distribution of soybean protein product irradiated by 60Co γ-ray with a pile-up irradiation technology was studied. The product bags were irradiated to half dose by the γ-ray source at two positions (low and high), and the second half dose was delivered in the same way to the product after position-change of the bags. Effects of the γ-ray irradiation, which included hygiene quality, physical and chemical index, functions and appearance of the soybean protein product, were investigated. The results show that decontamination of the product can be achieved by 3-5kGy of the irradiation, with improved utilization efficiency of irradiation source and high quality of the product.  
        关键词：Soybean protein product;Radiation decontamination;Double-source-position irradiation   
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        发布时间：2023-03-30
      • KONG Zhaolu,JIN Yizun,Chinje E C,Stratford I J
        Vol. 24, Issue 3, Pages: 159-162(2006)
        摘要：The study aims to evaluate radiosensitization of AQ4N, a new bioreductive agent, and to clarify the role of iNOS in its metabolism. It seemed that AQ4N was a hypoxia-selective radiosensitizer and mainly caused cells apotosis. iNOS-gene transfected cells showed G2/M phase block 24 hours after treated with AQ4N under hypoxia. The study showed that iNOS could be a target enzyme taking part in AQ4N bioreduction in anaerobic condition. Further work should be done to clarify the enzymology involved in AQ4N bioactivation to improve the application of AQ4N as a radiosensitizer in clinic.  
        关键词：Bioreduction;AQ4N;Apotosis;p450redutase;Radiosensitivity   
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        发布时间：2023-03-30
      • WANG Jianfeng,LIU Libo,WANG Tiejun,DONG Lihua,WU Zhenfeng
        Vol. 24, Issue 3, Pages: 163-166(2006)
        摘要：The work is to study radiotherapy-induced clinical symptoms of endoscopic findings, pathological changes and evaluation of esophagitis in the thorax. The clinical symptoms of 66 patients under radiotherapy were evaluated with the accumulated dose, endoscopic findings of esophagus and pathological changes. Typical symptoms in 2—3 weeks included moderate dysphagia or odynophagia and requiring narcotic analgesics with 20—30Gy of the accumulated dose. Endoscopic findings of esophagus were with erosion of mucosa or without sloughing, pathological changes of esophagus were the infiltration of inflammatory cells in squamous epithelium, disintegration of basic layer, deeply stained necrosis of cells. The grade≥2 acute esophagitis using RTOG criteria was observed 21.2%, and the grade 3 is 3.0%. The grade 2 and 3 acute esophagitis using Kuwaht’s criteria was observed 38.5%. The diagnosis of acute esophagitis should include history of exposure to radiation, with doses exceeding the dose threshold, and typical clinical characteristics. Endoscopic examination is very useful for diagnosis, since such examination can provide accurate information. RTOG scores correlate closely with endoscopic grading, and is convenient and precise. Pathology can distinguish between benign and malignant changes of ulcer.  
        关键词：Radiotherapy;Radiation injury;Esophagitis;Diagnosis   
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        发布时间：2023-03-30
      • XIE Yi,DANG Bingrong,ZHANG Hong,BING Tao,HAO Jifang,GUO Hongyun,WANG Xiaohu
        Vol. 24, Issue 3, Pages: 167-170(2006)
        摘要：For estimating biological risks from low doses irradiation, BALB/C mice were irradiated to 0, 0.39, 0.55 and 1Gy by X-rays at a dose rate of 0.2Gy/min. The immunocyte cycle and apoptosis were determined by flow cytometry, and the thymus and spleen weight were measured, too. The results showed that the cycle of peripheral blood lymphocyte, thymocyte and spleen lymphocyte were arrested in G0—G1. The apoptosis (expect for spleen lymphocyte) induced by the irradiation increased, whereas the thymus and spleen index declined with increasing dose. The results suggested that low doses X-ray total-body irradiated could result in changes of cycle and apoptosis, and some damages to immunocytes, and the X-ray irradiation affected the organs differently. It might provide experiment basis for radioprotection.  
        关键词：X-ray irradiation;Blood lymphocytes;Thymocyte;Spleen lymphocytes;Cellular cycle and apoptosis;Thymus and spleen index;Mouse   
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        发布时间：2023-03-30
      • KONG Zhaolu,JIN Yizun,Chinje E C,Stratford I J
        Vol. 24, Issue 3, Pages: 171-174(2006)
        摘要：To examine the effect of nitric oxide (NO) on tumour cells radiosensitivity, human sarcoma cells (HT1080) and its inducible nitrogen monoxide synthase (iNOS) gene transfected clones (iNOS9, iNOS12) were exposed to 1—5Gy 60Co γ-ray irradiation alone or combined with 500μmol/L L-NMMA, a NO synthesis inhibitor, in air or under hypoxia. It showed that both hypoxia and L-NMMA decreased the production of NO, but had no obvious effect on iNOS activity. With more NO formation, gene-tranfered cells showed higher sensitivity to irradiation and lower OER than HT1080, but there was no obvious correlation between NO and cells sensitivity to irradiation. The results indicated that NO could take part in irradiation-induced cells damage and enhance cells radiosensitivity.  
        关键词：Nitrogen monoxide (NO);Nitrogen monoxide synthase (NOS);L-NG-Monomethylarginine hydrochloride (L-NMMA);Gene-transfer;Radiosensitivity   
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        发布时间：2023-03-30
      • JIN Cailing,XU Yujie,FAN Wo
        Vol. 24, Issue 3, Pages: 175-177(2006)
        摘要：To estimate absorbed doses by 123I-AnnexinV in human, 125I-AnnexinV was used as a radiotracer for measuring the distribution of radiolabeled Annexin V in mice. The standard Medical Internnal Radiation Dose (MIRD) method was used by Mirdose-3 software in dosimetry estimation. The results show that liver and kidney received 2.77×10-3 and 2.71×10-3 mGy/MBq, respectively. The red marrow received 1.78×10-5 mGy/MBq, and the other organs received doses between 1.5×10-4 and 10.5×10-4mGy/MBq.The effective dose was estimated at 5.55×10-4 mSv/MBq. Human radiation dosimetry can be performed by the mice biodistribution data and important data for clinical safe trial of 123I-AnnexinV are provided.  
        关键词：123I-AnnexinV;Mice;Radiation absorbed doses   
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        发布时间：2023-03-30
      • ZHOU Zhou,ZHANG Lei,DENG Zhaohui,YU Xiaodong,ZHANG Guangbin,YU Zhengping
        Vol. 24, Issue 3, Pages: 178-182(2006)
        摘要：The work is to determine the effects of γ-irradiation on GSK3 β coupled signaling transduction pathway in IEC-6 cells, and protect effects of regulation of GSK3 β and caspase-3 activation onγirradiation induced IEC-6 cells injuries. The changes of GSK-3 β and caspase-3 mRNA expression were determined by RT-PCR. The change of GSK-3 β phosphorylation was determined by western blotting. The changes of GSK-3 β enzymes activity were tested by radiation activity measurement. The changes of caspase-3 enzymes activity were assessed via a colorimetric assay with specific substrates. The IEC-6 cells apoptosis were determined by Hoechst 33342 staining and DNA ladder. The results show that 6Gy γ-irradiation increased apoptotic cell percentage in IEC-6 cells. Significant changes of GSK3 β and downstream signal transduction molecular caspase-3 in mRNA expression, GSK3 β protein dephosphorylation and protein enzymatic activation were observed after the irradiation. GSK3 β and coupled signal transduction pathway may play an important role to promote IEC-6 cells apoptosis. Inhibition of GSK3 β activation can provide protective effects against the irradiation induced IEC-6 cells apoptosis. Pretreatment with caspase-3 specific inhibitor has similar effects on IEC-6 cells, being characterized by decreasing apoptotic cell percentage and blockade of DNA fragmentation induced by the irradiation. GSK3 β and coupled signal transduction pathway plays a very important role in the irradiation induced IEC-6 cells injuries. Antagonism of GSK3β and caspase-3 activation may be an important approach to protect intestinal epithelial function. These results are of clinical relevance in antagonism of the irradiation induced intestinal dysfunctions.  
        关键词：γ-irradiation;IEC-6 cells;GSK3 β;Caspase-3;Apoptosis   
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        发布时间：2023-03-30
      • NING Ping,LIU Fenju,XUE Jing
        Vol. 24, Issue 3, Pages: 183-187(2006)
        摘要：To study radiosensitizing effect and apoptosis induction of tamoxifen on human glioma cells, 3H-TdR incorporation assay, flow cytometry, laser confocal microscope and Immunohistochemistry were used to investigate the inhibition effect on DNA synthesis, apoptosis and estrogen receptor expression. Experiment results suggested that tamoxifen could inhibit DNA synthesis in SHG-44 cells,the combination of tamoxifen and 60Co γ-irradiation showed a synergistic action on it. Tamoxifen could also induce the apoptosis, and the apoptosis rate increased along with tamoxifen concentration was elevated, so morphologyical changes, such as karyopyknosis, nuclear fragmentation and apoptotic body were observed. However, SHG-44 cells did not express estrogen receptor. The above results revealed that tamoxifen can sensitize SHG-44 cells to gama radiation, which might be related with apoptosis pathway instead of the estrogen receptor-mediated process.  
        关键词：Tamoxifen;Glioma;Radiosensitivity;Apoptosis   
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        发布时间：2023-03-30
      • WANG Dan,REN Shaoxiong,LI Weifeng,SU Qianzhi,SU Jun
        Vol. 24, Issue 3, Pages: 188-192(2006)
        摘要：Celosia seeds were irradiated by 3MeV electron beams or implanted with 30keV N+ or H+ ions, The results showed that the two kinds of treatments could inhibit growth and development of the plants and induce variation of the flower. The variation rate was between 0.5%—2%. LD50 of the seeds irradiation by the E-beams was estimated at about 1.2kGy. LD50 of the seed ion implantation varied with the ion. LD50 of H+ ion implantation was 1.6×1017 ions/cm2, and LD50 of N+ ion implantation was less than 1.6×1016 ions /cm2. Suitable treatments of the seeds were 1.5kGy E-beam irradiation, or implantation of1.6×1016 N+ /cm2. The seeds were not sensitive to higher dose irradiation or ion implantation. And it seems that Celosia seeds have strong radiation resistance.  
        关键词：Celosia;Electron beam;Ion implantation;Dry seeds;Growth and development   
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        发布时间：2023-03-30

        研究论文

      • JIANG Zhigang,ZHANG Jie,FENG Shengyu
        Vol. 24, Issue 3, Pages: 141-144(2006)
        摘要：Effects of five kinds of aromatic additives (biphenyl, naphthalene, phenanthrene, benzophenone anddiphenylacetylene) on radiation resistance of heat-curable silicone rubber were studied. The changes in average molecular weight between crosslinks (Mc) and mechanical properties before and after γ-ray irradiation of 400 and 600kGy in vacuum were analyzed. Protection mechanisms of the aromatic additives are discussed. The results show that the aromatic additives, especially diphenylacetylene, can improve radiation resistance of silicone rubbers.  
        关键词：Heat-curable silicone rubber;Additive;Radiation resistance;Radiation protection mechanism   
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        发布时间：2023-03-30
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