摘要:Polymer modification as cross-link, chain scission, and graft-polymerization by radiation is initiated by the quantum energy transferred from radiation to polymers. The active species for chemical reactions are produced through ionization or activation of polymer molecules for any radiation source. The energy transfer occurs mainly by ionic interaction between radiation and polymer molecule, and the contribution from the collision interaction is miner. The radiation of electromagnetic wave as X-ray or γ-ray generates the energetic electron which induces ionic interaction with polymer molecule. The energy loss profile along the penetration to polymer material is much different among the radiation sources of EB, γ-ray, and ion beams in the macroscopic mechanism. In this article, the behavior of single event, that is, the event induced by one electron, γ-ray, ion, and neutron is described by the macroscopic mechanism and by the microscopic mechanism.
摘要:Polymer modification such as cross-link, chain scission, and graft-polymerization by radiation is initiated by the quantum energy transferred from radiation to polymers. The active species for chemical reactions are produced through ionization or activation of polymer molecules for any radiation source. The energy transfer occurs mainly by ionic interaction between radiation and polymer molecule, and the contribution from the collision interaction is minor. The radiation of electromagnetic wave as X-ray or γ-ray generates the energetic electron which induces ionic interaction with polymer molecule. The energy loss profile along the penetration to polymer material is much different among the radiation sources of EB, γ-ray, and ion beams in the macroscopic mechanism. In this article, the behavior of single event, that is, the event induced by one electron, γ-ray, ion, and neutron is described by the macroscopic mechanism and by the microscopic mechanism.
摘要:In order to investigate the feasibility of electron beam induced mutation on Gladiolus and the effects of electron beam with different doses on pollen traits, M1 generation pollen traits were studied by use of 3MeV electron beams with seven doses of 0Gy, 40Gy, 80Gy, 120Gy, 160Gy, 200Gy, 240Gy. It has been shown that the sterile pollen rate of M1 generation is higher than that of control. At low dose there had no obvious effects on the viability and germination rate of M1 generation pollen. With the increase of irradiation dose, the viability and germination rate of pollen was decreaced and the difference had statistical significance (P<0.05). Comparing (SEM) photograph of the pollen shapes of M1 generation with control, within a certain doses level, mutagenic effects on pollen increased with the dose increasing in the angle of polynology. Variations of mutagenic effect extent were found at the different irradiation dosage. The EST isozymes expression of M1 generation pollen was obviously changed by electron beam irradiation confirmed by use of PAGE electrophoresis. EST patterns of pollen of Gladiolus irradiated at the dose no more than 120Gy were similar to CK, but there were some differences in the vigor of isozymes. The EST bands added or absent had been observed at the dose more than 160Gy compared to CK. There were a special EST band more and 2 EST bands less in plants treated by both 160Gy and 200Gy electron beam irradiation, and a special EST band more and 1 EST bands less in plants treated by 240Gy electron beam irradiation. The vigor of isozymes of plants treated at the dose more than 160Gy showed some respective differences compared to the other treated and CK's. These results revealed that electron beam irradiation is an effective way for Gladiolus breeding.
摘要:The three ferrocenylhydrazone coordinated metal polymers were synthesized (PZM). The effect of the 60Co γ irradiation on microwave dielectric properties and their temperature-dielectric properties were studies. It has been found that the dielectric parameters (ε’, tgδ) of coordinated polymers increase along with the absorbed doses and coordinated metals in order Cu, Co, Ni, However, the dependent curves of dielectric parameters on arise-down temperature are universal. On the other hand, the small changes in chemical structure before and after irradiation were confirmed by IR differential spectrometry and SEM. It is possible to make such coordinated polymers as a multifunctional polymeric material with optical, electric and magnetic properties, which may be potentially used in microwave communication.
摘要:In order to study biological effects of12C6+ heavy ions irradiation on Allium fistulosum L., the dry seeds were treated by 12C6+ heavy ion beam with different doses. Obvious "parabola" trends were observed in the characteristics of germination rate and height of Allium fistulosum L. seedling when irradiated doses increased. With irradiation of an appropriate dose of 12C6+ heavy ion beam, 30Gy, the germination rate of Allium fistulosum L. is increased and the seedlings can resist drought and lodging, and grow better than the control. At the same time, the formation frequency of micronucleus and chromosomal aberration were surveyed in root-tip cells. The highest formation frequency of micronucleus and chromosomal aberration treated with 180Gy could reach to 9.09% and 10.03% respectively. This study laid the basis for further work on breeding and improvement of Allium fistulosum L. irradiated by 12C6+ heavy ion beam.
关键词:12C6+ heavy ions;Allium fistulosum L.;Chromosomal aberration;Biological effects
SONG Hongyan,HE Suqin,LIU Wentao,YANG Mingcheng,ZHU Chengshen
Vol. 25, Issue 4, Pages: 216-220(2007)
摘要:In this work, the thermo-sensitive hydrogels of P NIPA/Clay were synthesized by 60Co-γ rays irradiation. The effects of organically modified clay and Na+ clay, clay content, and dispersing condition on swelling behavior of PNIPA/clay hydrogels were investigated. The results showed that the equilibrium swelling ratio (SR) of the PNIPA/clay hydrogels is better than PNIPA, and the SR of PNIPA/ organically modified clay hydrogels is the highest. With clay content increases, the SR of hydrogels became better. The deswelling behavior of hydrogel was improved, the deswelling ratio of the hydrogel with organically modified clay is highest, and ratio of losing water is 83%, while PNIPA is about 50%. The compressive properties of hydrogel composites were also examined. The results showed that the compressive properties of the PNIPA/clay hydrogels were improved distinctly than that of the conventional hydrogels without clay. And with increasing of clay content, the compressive properties of hydrogel composites improve rapidly. When the content of clay is 15%, the maximum compression force of the PNIPA/clay hydrogel is 5.28N, which is 14 times of PNIPA hydrogel and compression strength is 2.5 times.
摘要:To study root curve induced by 241Am α-particle irradiation on Arabidopsis embryos, molecular probe assay, outside-H2O2 assay, ROS-extinguisher assay and root measurement assay are adopted. The study shows that root curve angles induced by 241Am α-particle irradiation on Arabidopsis embryos have a positive relation (r=0.98) with irradiated doses within 100Gy. Root curve angle of 1Gy, 10Gy, 100Gy, and control is 13.3°, 28.7°, 69.1°, 12.5° respectively. Root of 100Gy treatment shows an "S" type curve while root growth is restrainded. Pseudo-color images of CM-H2DCFDA, which represents quantity of ROS in root tip, shows brighter in 100Gy irradiated group than that in the non-irradiated group. Root length and root gravitropism recover occurs when suitable concentration of scavenger of ROS is used. And root curvation has been observed while asymmetric replacement of H2O2 used. The study indicated that α-particle-induced the change of root growth and root gravitropism might be due to ROS.
关键词:α-particle irradiation;Arabidopsios embryo;Root gravitropsism;Reactive oxygen species
摘要:The effect of static magnetic fields (SMFs) on the chromosome aberrations in the pollen mother cells (PMCs) of wheat was studied. The seeds of wheat were exposed to static magnetic fields of different magnetic flux density (0, 1, 3, 5 and 7 Tesla) for 5h and with different treated time (1, 3 and 5h) at magnetic flux density of 7 Tesla. The genotoxic effect was evaluated in terms of micronucleus (MN), chromosomal bridge, lagging chromosome, abnormal segregation and fragment in PMCs. The results indicated that the exposed groups of low field intensity (below 5 Tesla) showed no statistically significant difference in aberration frequency compared with unexposed control groups and sham exposed groups, but significant differences of chromosomal bridge at 5T and lagging chromosome, triple-polar segregation or micronucleus at 7T were detected in the exposed group (p<0.05). The dose-effect relationships indicated that the increase frequency of meiotic abnormal cells correlated with flux density of magnetic field and treated time, but no linear effect was observed. These results lead us to the conclusion that high static magnetic fields above 5T for 5h duration might cause an increase in potential damage to organisms.
关键词:Chromosomal aberration;Micronucleus;Static magnetic fields;Pollen mother cells
摘要:About a new mutation breeding method which combines plant tissue culture technique with heavy ion beam irradiations were discussed in this paper with the principles, operation steps, molecular mechanisms, etc. The mutation methoddeveloped a few advantages coming from plant tissue culture, which can produce offspring by asexual ways. Meanwhile, using this method, the study of biological effects of high energy particles with different linear energy transfer values on plant tissues or cells can be explored and optimized in theory or practice.
关键词:Ion beams;Plant tissue culture;Irradiation;Mutation;Linear energy transfer
摘要:The effects of short-term irradiation with low dose rate γ and β rays on the electromagnetic parameters of InSb Hall transducer at different working states, such as input resistance, output resistance, magneto-resistance and Hall sensitivity etc, were systemically studied. The results indicated that the action of γ or β rays with ionization and displacement effect on Hall devices could result in the complicated changes of electromagnetic parameters. These changes didn’t disappear rapidly after irradiation stopped. It reflected the radiation damage could not be restored through annealing courses at room temperature. When the devices were driven by constant current and not in any magnetic field, their input resistance linearly increased with irradiation time. When the devices were driven by constant current and in certain magnetic field, their input resistance and Hall voltage could increase along with γ irradiation, but the extent was less and the direct proportional relation with irradiation time didn’t exist on account of the action of magnetic field. When the devices were not at working state and not in any magnetic field, their input and output resistance all increased with irradiation, but their magneto-resistance and Hall sensitivity might increase or decrease. All these results could give some helps for the relevant studies of radiation defense at some extent.
关键词:Irradiation with γ and β rays;InSb Hall Transducer;electromagnetic parameters
摘要:The dose deposition of different parts of Aspergillus oryzae spores were analyzed with soft X -ray energies near the K-shell absorption edges of C, N, O elements (4.4nm, 3.2nm and 2.3nm), respectively. At the same time, the spores were irradiated with the three wavelengths of soft X-rays on the soft X -ray microscopy from synchrotron radiation at NSRL, and the survivals were compared. The theoretical analyses showed that the deposition doses of different parts of the spore were varying with X -ray energies because of the effects of C, N, O K-shell absorption edges and elemental contents of the different parts of spore. The experimental studies proved three wavelengths of soft X -rays all had high killing abilities. Among these, 2.3nm wavelength X -rays had higher radiation damage to spore than that of 3.2nm, 4.4nm.
摘要:Cell culture was carried out to investigate whether the protective and repairing capacity of SOD against the radiation damage induced by UV-C was improved because of its transduction ability. When L-02 cell line being exposed to UV-C in the solution with SOD enzymatic activity of 320U/mL, the protective rate of PTD-SOD increased to 58.5% , which was about 5 times of that of wild-type SOD; when irradiation duration prolonged to 140s, the protective rate of PTD-SOD remained to be 43.2%, while that of wild-type SOD was almost undetectable. Regarding restorative rate, that of PTD-SOD could reserve 50.1% when L-02 cell exposed to UV-C for 40s, while that of wild-type SOD was less than 8%. These results suggested PTD enhanced the protective and repairing ability of SOD against UV radiation remarkably, owing to improve bioavailability of SOD.
摘要:The screening of radiosensitive genes of human colorectal cancer was made by gene chip. Two human colorectal cancer cell lines LOVO and SW480 were cultivated and the total RNA was extracted from at least 1×107 cells. Then the gene expression profiling was performed by HG- U133 Plus 2.0 Arrayand the differenceof gene expression has been analyzed. The results shows thatthere are 16882 genes expressed in LOVO cell and 17114 genes expressed in SW480 cell through gene expression profiling.It has been found that the genes with 2-fold expressed differentially include 908 genes up-regulated and 1312 genes down-regulated. The same genes, such as Fas and NFkB which is up-regulated, Caspas6,and RAD21 which is down-regulated, have been proved to be related to radiosensitivity. The genes with high expression level including CEACAM5, THBS1, SERPINE2, ARL7, HPGD in LOVO cell may also be related to the radiosensitivity. And the genes with high expression level including SCD, NQ01, LYZ, KRT20, ATP1B1 in SW480 cell may be related to the radioresistance of human colorectal cancer. It could be concluded thatthe radiosensitivity of colorectal cancer can be reflected from gene and protein expression level. And gene expression profiling is a fast and sensitive tool to predict the radiosensitivity and screen radiosensitive genes of colorectal cancer.
关键词:Colorectal cancer cell;Radiosensitivity;Gene chip