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PAN Yan; GAO Gang; LIU Lantao; RUAN Jianlei; LIU Jianxiang; SU Xu

doi:10.11889/j.1000-3436.2014.rrj.32.020201

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更新时间：2021-12-01

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- Journal of Radiation Research and Radiation Processing Vol. 32, Issue 2, Pages: 20201-020201(2014)
- 作者机构：
  
  1. （辐射防护与核应急中国疾病预防控制中心重点实验室中国疾病预防控制中心辐射防护与核安全医学所）,北京,100088
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- DOI：10.11889/j.1000-3436.2014.rrj.32.020201
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PAN Yan, GAO Gang, LIU Lantao, et al. [J]. Journal of Radiation Research and Radiation Processing 32(2):20201-020201(2014)
DOI：

PAN Yan, GAO Gang, LIU Lantao, et al. [J]. Journal of Radiation Research and Radiation Processing 32(2):20201-020201(2014) DOI： 10.11889/j.1000-3436.2014.rrj.32.020201.

摘要

采用流式细胞术检测60Co γ-射线照射后γH2AX表达的量效和时程关系，同时探讨紫外线联合60Co γ-射线照射后γH2AX的表达情况。结果表明，不同剂量60Co γ-射线照射后γH2AX表达量与照射剂量之间呈现明显的剂量效应关系。4 Gy 60Co γ-射线照射后，γH2AX的表达在1 h达到峰值，随后逐渐减少。与假照组相比，单纯紫外线照射组γH2AX表达增加，一直稳定维持约6 h左右，随后开始减少。紫外线联合60Co γ-射线照射组与单纯60Co γ-射线照射比较，γH2AX的表达无显著性差异。结果提示采用流式细胞术检测γH2AX的表达估算受照剂量具有可行性，该法有望成为估算辐射剂量的新方法。

Abstract

Flow cytometry analysis method was used to detect the changes of γH2AX protein expression in human peripheral blood lymphocytes. In the dose-effect study, peripheral blood was irradiated by 60Co γ-rays with the dose of 0-6 Gy and cultivated for 1 h. Peripheral blood were cultivated for 0-24 h after 4 Gy 60Co γ-rays irradiation for the time-effect study. Then, the peripheral blood was divided into four treatment groups, i.e. UV irradiation, 60Co γ-rays irradiation, UV plus 60Co γ-rays irradiation and control group to detect the changes of protein expression in different time. The results showed that the γH2AX protein expression was in a dose-dependent form. The expression of γH2AX peaked at 1 h after 4 Gy irradiation and began to decrease quickly. The expression of γH2AX in UV irradiation group increased compared with that of the sham-irradiation control group, and the expression of γH2AX peaked at 6 h. While compared with that of the 60Co γ-rays group, the γH2AX expression of UV combined 60Co γ-rays group was unchanged. The results reveal that the detection of γH2AX protein expression changes in peripheral blood lymphocyte by flow cytometry analysis is reasonable and may turns be a new way for biological dosimeter.Cited

关键词

60Co γ-射线γH2AX紫外线DNA损伤生物剂量计

Keywords

60Co γ-raysγH2AXUVDNA damageBiodosimeter

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DOI：10.11889/j.1000-3436.2014.rrj.32.020201

摘要

Abstract

关键词

Keywords

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